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CRIMIC CR00925

Gene stum

CG30263

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:21683164..21683173	57F8	FBti0199690	79367

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAGGTCCGTCAAGCCACTCATAAGT	ACAGAACCAGGCTAAATACAGCGGCA

Images

Construct Design Sequences

sgRNA sequence	GTCCTTGGCTATCAGGCGACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGTACGGTATAGCGTGCG	GGCGACCGGATATAGGCTTT	AAGGACAATGGGTGGGCAAA	CCTCGACGACATGGTATGCA

 

Amplicon 5'

Amplicon 3'

ACTGTACGGTATAGCGTGCGCGGAAAACAGGAGAAAAGTATTTTCGCAACACGGCTTAAGTTTCTTGCCGCAATAACAAATCAGTTCGGGCCGGACAATCGCGCCAACCGCAGCAACTCACTCGCTGGGAAATTCCAGTTCGGAAAGTTGGAAAAGTCCTCGCCAGTCGATGATTTTCCCAGCAATTAATACGAAATAACAATTCAGTTAATTTTTGCCAATTCGAAATGCTGAACGGAACGCCGAATAACGGGGTCATTTACACGGTGGATCTGGCCCAGGATCCCTTCATCGATGGCGACAAGTACTTCTTTAGGTAATTAGCCACGAGATTCAATTATAACTTATCGCTGCTTGTCATCTGGCAGCGGGTAAATAAACGCACTGACCTTCTGGCGGCATTTCGGGCACTCCGATTCCGACTTCAAGAATGTGCGGAAAACTTGCTCCATTCATTTCGACTGTCTCGCAGTTTTGTTTGAATTTCGACCGGAGCGGGTCAAATAAATTGCCGACCTCCAAATTGCGTTTGGTCGTTGGCTCGTGCACAACGGAATTACTGGCCTGCTTGTTCAGGCACATATATATATATATATATATATTATATACACGTGAACTTCTTCTTGAGCTGCGGGCATGGACCCATATAGAAATTGTGTAAGCAAAACTCTCGTGACACATTTTGTTGGGCTGGCATACTCGAAATCGACCCGAAAACGAAACTTCATTATGCCCGGATGAAAGTTCAAAAGCCACAGATTTAGCATTACACGCTACTAACTAACTGCTGCAACTGCATTATTCATAACTTTGAGATACTAGATAAACAACACATGCGCCATCCGTAGGCCCACGCTAATGGTAATTCACTTCCGAAGGTGATTATTATATTTCACGCCAGCGAAGTGCGGAAGTGATTCAGGACTATCCCACAAACTTGAACTTTCCAATCATATAGCACTTCCGCCCGCCTGGCACTTGGCCAATCCTCGTAGCCATAAAAGCCTATATCCGGTCGCC

AAGGACAATGGGTGGGCAAATTACTTTACACCAAGTCATAATCGGACTTAAAGAAATTGAAGGACTCTCTCGAATACTTTGGTGCTTTAAAAGCAAGAAGGTTTATCAATAATACACATTTAAAGTCCGCGTAGAAACTAACACGACAGTTTCCTTAATTTAAGCCTTAATTGTATTTCCCCCTTCCCTCTCCTTTCCTTTCAATCAGGAGCAGCAGTCAGGAGTTCCTTGAGGACAAGGATAATGTCAAGAAGGGCGGTCTGTGCGGATGCTGCTCCAAGCTGTTCATGCCCTGCCGCCGATCCCGCTGCTCTCGTTGCTGCCGGCGCCGTGAGCGAAACGAATCCGCCGAGGATCAGCCTGTCCTGTGGAGCGGCAACAGTAGTGCCACCACGGCCACCAATGTCCAAGTGATTGACGAGACCAAGCCGAAGCGCAAGAAGATATCGGATTGGCTAAAGTCCAGCTGCTGCCGGAGCTGTCGCAAGAAACCAGCCATCGAGGAGCACGAAGCACATCAGGAGGAAGTGTCCAAGCGGACGAACCAGGCGGCTAACATGAGCACGGGACCAAGGACACGAGGCAAGTGCGGCCTCTGTCTGAGCAAGGTGTTCTGCTGTCGATCGGTGAACAGGGTGGATCCCACCACCGGCGACGAGACGGAGATGAAACAGTGCTGCTTCTGCATACCATGTCGTCGAGG

 

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