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CRIMIC CR00927

Gene CG30460

CG30460

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:17071661..17071661	53F5	FBti0199691	79362

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGGACGCATCCACAGCAACAAGCA	GATTTCTCTGCATGGGCACTAGGCTA

Images

Construct Design Sequences

sgRNA sequence	CCTAACCCCCGATGCTCTACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAACCCATACCTTTGCGCT	CCGATGCTCTACTGGCGTT	GCATCGGGGGTTAGGATAAC	CGTCTAACTCGACACCGGAG

 

Amplicon 5'

Amplicon 3'

TCAACCCATACCTTTGCGCTTGATTAACCCATTGAGCTCGTCAAATGTCATGGTTACTTGGGGAAGGCAGATTTGCTTTTCGACCGCATCTAGTTTATCCGTCTTGGGCCCTCTCTTGCGTCGTCTAGCTGGCTGGAGAACTGGCGCTTCCACGCAATCGAGTTTGGCCAACTGGAGAGCATCTCCACTGCCGTTCCGCCTTAGTTGTTCCATGGTCTCGTCAATTTCTATGCAGGATTCCCGCTGAGTGCGCTTGGAACGGCGCAGTGCAGATCGTTCCCTGGGCGGCGGCGATGATGGTGGATCCTCATTGAACAGGTTCACTTGGTTCTGGGTAACCGTGGAGTTTGCTTTGGCACGACTTCCTCCTCGCGAGCTGGAGACTTGAGCTTGAGTGCTGAGGATCATTTTGGGGATGAGTGAAGATCGCTGATCGAAGAACTAACTACGGTGTGTTGAGACTACACGACGGTGGCAATAATTGCTATCAAAAGGTTACGATTACGATTACGCTTTACGGGATTAATCAAGGACGACTAAGATAACGCCAGTAGAGCATCGG

GCATCGGGGGTTAGGATAACCTATCTAAGAGAAATCTATGGATTTCGGAAAACCCCTACTCACTTATCACTGTGCACGGACGACGTCATCGAATGGGACATCAGGTTTGGTTTGGGTGTTGTCATCTCACCCGCTTCCAGAACCTCCTCGTGGTTACCGGAGTACTTATCCCTGGCGAGGGATCTCTTGGTCCTGTCAAACATCGCGGTCAATTATATAATAGCAACAAAATAAATTGAAACGAGAAAGAAAACAAAAACGTGTGTTAAGTAGATAGATCACAGGGTGTCAACTAAATCCAGGCTTACTTCAGATTGGGCGGCCTGTACTGCTTCCTGGCCTCCAGGCGCTCCTTGGCTTTGCTGGTCTCCTGGCTGTTGGTGGACTTCATGGTGGCCTGAATGGCCTTTTCCTTACACCATTCTACGTGGCGATCAAATGCCTTGGGATTAAAGGTACGATCGCAGTGGGGACAACGATCACAGGGAGGTGCCTTGATCCGCTTGGCCAGCGATCGTTCGCGGACAAGAGGAGCAGCTGGAGCAGCTGGAGCAGCCGGAGCAGCTGGAGCAGTCGAAGCAGCGGGTGCCATAGCTGGAGCCTCCGGTGTCGAGTTAGACG

 

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