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CRIMIC CR00929

Gene CG31650

CG31650

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:5041637..5041643	25C1	FBti0199692	79363

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGCAGGCGAAAGGTGAACTGAGTT	CGGGGCTTCAAGTGCTTGCTAGAAAA

Images

Construct Design Sequences

sgRNA sequence	TGCTGAGGTGATTATTGGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTGATCCTCATCGGTGGAG	TCACCTCAGCAACCTAATGC	GGCTTGGCTAACATTGATGCA	GGGTCAAGGACGGCATCTAC

 

Amplicon 5'

Amplicon 3'

CGTGATCCTCATCGGTGGAGACAAAGAGATGGTCCACCTCGTCGTTGGCAATTGCCGTATTGCTTGGCACAATCCAGGATAAGACCTCATCGCCCGTCAGCACGCCATCGCCATTGGAGTCGTGATCCTTGTCGAAGCGCTCCTTCTCCGTGATCAGCCACTCCTTGTCGTGGTGCGAGGCTGCGTCGCCGACAAACTCCTGGAAGTTGATCTTGCCATCGTGATCCGCATCTTTGTCCTGCATCGTGTGCTCCAGCAGTATTGGCAACATTTGTGGATGCTCCTCGGGATTTTGGAATAAAACGAATTCCTCCAGCGTCAGCACACCATCCTTGTTCGTGTCGGCCGCATTGAACATCTCCTTGTCCTGCTTGATCATCTTCTGCTCATCCTCGTAGCTGTCGTAGTCGATGGTCTCCTTCTTGAAGTCCTCATCCTCCATGGCGTATGTGTCCTGGAGATACTCCTTCCACGTAATTCTCTCGTCCGCATCCTGGTCTATTTCTTCAAATCGATCAGCGGCTTCTTCCTCCGACAGTTTTCTGCAAGTTGTATTATGTATTTTAGTAAAAGCTTCAAATAGTCTATGCGTTTTCAGTTTAGGCAAGCATTTAGACAAAGCATTAGGTTGCTGAGGTGA

GGCTTGGCTAACATTGATGCAGTATTAATCAACAGACAACACTCACTTAAAGGACCTTAATATCCAGGCTTTCAACTCATGCCGGTCAATAAATTCGTCCTTATTCAGATCCATCATTTTGATCAATATCAACAGTCGTCGCTTCGATTCGTCTGGCGAAAGTGAGTCGAATTCCTGAGCCTCTTTCGTGTTGCCTTTTTTGTGGTTAAAGAGTAATAATAGCACACACATACATTAGCATTTAAAACTGAATGGAACATAAAACAGCAGCAATGCCATTGACGTATTGCTTTTAGAAAACAATTTTAATGATTTCACTATAAAACTATACAAACTTATATACTTCAATAATACATTTTCTTACAGCATATTCCAATTCATTAGTAAACTATATTTCGACCTTCAAGCAATATTTTTGAGCATAAATGGGTATGTGTATTGACTGATCCGTATTCTCTTGATGCTTGAATGAGTCTTTCCGTGTGCAGACCCACAAAAATAAACCTCAGGCAAGTAAACAAACATATGGCATACACCATAGCATACACAAAAAAAAAGTATGAGTCCTTTTCATAACTAAAATAATACAAAATTATGGTCAATACTCATAATTTCATTGGGTTGGAAAGTATCCTTAAAATAAACTTTTATATTTCTACAAATTAACAACTGAAACTGGAAATTCTATTGTTGTTCTGGCAAAAAAACGTGATTATATTTTATTTGCTTTTCAGGAAATGAGCACTACTTTTATGACCTTCATTGTAAGGAGTCTTTTTCGCTCTTTTCAAAGTGATTTTCTCAAACTGAATATGATCTGCTTTCTCTGCTAACTAGCGCTGACTCACCGATGATGGCCTCGTGATCGAACTCCACGTTGTGCTCCCCATCCTCGCCGTGGTGGTGGGCATCCCGGGGAGCGTAGATGCCGTCCTTGACCC

 

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