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CRIMIC CR00930

Gene kek3

CG4192

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:15581086..15581114	35D3	FBti0199693	79364

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGGCTTGGGATAAGCTCGATGTAGA	CTATCCGAGGAGTTGCGGAAGTACTT

Images

Construct Design Sequences

sgRNA sequence	GGGACACGGACGCCCGGTCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTCTCACGGCTGACATGCA	TCGAAGTAACGAGAACCGCA	CGTGTCCCTGTCAAAGAGCT	TCGGTGCTGGTGAAAGGAAA

 

Amplicon 5'

Amplicon 3'

ATTCTCACGGCTGACATGCAGGATGCCGGGATCTATACGTGTGCGGCGGAAAATAAGGCTGGAAAAGTGGAGGCCAGTGTGACTCTGGCAGTATCCCGTAGACCCCCGGAAGCTCCGTGGGGCGTAAGAATTATTCTGCTGGGGGCGGTAGCCGCTCTGCTCCTCGTCGGTGGATCCTCCTTTGCGGCCATTTGCTTGTGTTCCCTACAAAGGCGAAGAAAGCTGCGTCTCTGGAACTCTGTACCTCCTGTGAGGAGAAGCGAGAGCTACGAAAAGATCGAGATGACGGCCAGAACGAGACCGGATCTGGGAGGAGGGGCTAGTTGCGGAGGCGGCAGTGCCACGGGCGCCGGACTCTTTCACGATGCCGAGGAGCAGGGCTATCTGCGGGCAGCTCATACGCCACTAAATGACAACGATGCCGGGCAGGCGGCGGCCATCGTAAATCCGAGTGCAGGAAGTGCACAGCGAAGAAATGGAGACTACCTGCACGTGTCCACCCACTGCGATGATGAGGAGGAGGACCAACAGCTGCATCACCACCCACAACAGCAGCCCGCGAGCCAGCACCACCCACATCCCAATCAGCAGCAGCATCAGCAAAGGAAGGGCTCCCAGGGCCATGTTGTCTCCGCATCCGGGGCGAATAATTCAGCACCGCTGGAGGAAACGGATCTGCACATACCGCGCCTCATCGACATCGGCGGGTAAGTTTTCCAACACCGGGCAAAAAGTGTGAGGGAAATTCTTGAAATTAAAAACATGTAAAAATTTTTTGGGCTTATTTATATCTTGAATTTCGTTTGTAGGTTTTGAATATATCTTTAGATTTTATTTAAAGCTTAAAATGTACATCTTATATTAGCTCATTACCATTACCATGATTTCTTCACTCAGTGTATGCCTCGGGTGCCCTTTGTTTCTGCGGTTCTCGTTACTTCGA

CGTGTCCCTGTCAAAGAGCTGTCATGCTCGAGTGCGGAACGCGCCCGGATCTCCGTATTGCGTCCGTATTATGGGCAACACTTGACACTTCCACTCTTGGCTCGAGCCTTCTTTTTGAAAAACCCCACGCCCATTGCCCACCAGCCATCCGCCTCCATCTCCGGCGACTATGAGAACGCGCGTGCTAATGACGCGCGAAATATGCGAGCACATCCACATTCAACGTCCTGCCCCCCGCACCCAACCTTCAGCATCCAAGACCACATCCACATCCTCATACACGCGATCCGGAGAAGTGGTTTATTTCGGGCACACTAAGAACTCACTCTGCCTGATTGGCGCTTGTCATGTAAAAGGAATCCTGCCGCGGATGCGAGTACGAGTGCGGGTTAATGGATCTGCGGTGCCGGTTATCTTCATGGACGATGACACTGCCGAATAACGACAATCCGAAAAGTCAAGCACTTAAGCAATATAGGATGCAGGCAATTACTAGGGCTGTGAAAACAAAGGGGGACAGCTCATTCAACTGGGCAGGAATAAAAAATGTTTAATTTCTTTTTTTTTTTAAATCAAAAATCGTAAGCATTCAAATGTAGCCAAAATGTATACTTGGGTATTTCCTTGTATTTTTTTATTAGCAGTTTATAGAGTTTTATTTTACTTTTTTTTAAAATATTTTTTTCCTTTTTCTTGAGTCCCACAAAAATTAAAATGGTATCTTTTCTTCTTTTCCTTTCACCAGCACCGA

 

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