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CRIMIC CR00933

Gene dac

CG4952

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:16478654..16478654	36A1	FBti0199694	79365

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCAACAATAGCGGCGACTGTCGAAT	TGCAATCTGTCGACCAGAGACCAACT

Images

Construct Design Sequences

sgRNA sequence	TTTCCCTGAGACCTGCCCTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGTGAGGAGGAACCGGTTG	TGAGACCTGCCCTTTGGATC	GGCAGGTCTCAGGGAAATGTT	CAACATTGTGGCTGCCTCAC

 

Amplicon 5'

Amplicon 3'

GAGTGAGGAGGAACCGGTTGCCAACACTGGCCACACAATTCTCGAACCAGTTTGCAGCTGTCTCCTTGCGTCTCCACAGCGATCGTACATACATACATATGTACATACGAGTATATTTCAATAATCAAGGGATAGCTGAGTTGTTAATTATTGACTTGTGGCCGGGACGCTTGAGAAACAGCCGCCAAAATATGCGCCAAAGTGCGCTAAATGTGCCACTGCCACTTCACTTGCAGTTGCAGTTGCATAAATCGAATCGAATTGAATCGATCCGCATCGAATCGTATCGAGTCTTACCAAATCGAATAGTTAACATCGGGCTAGGAAGGCGTTCCCGGCATTTGTCAGCGAATCCACACTCCCTGGGCTTAACCCACTGCCCCCGGCGTTTTGGCGATCTGGCGTCCAGAAACTGGGGCTAAGCTGCAAATTGGCAAATCAATTATATTTATGGCCAAGTTGCCACCTTGCGACCGCGATGCAGAGCGTGCTTGCCGGCAACTAAATCAATGCAATCCCAGTAACCGACAATTAAATGTGTGCCTGCAACACAATTTCCATATTTGCAATTACCAATGCCGAGTGCTCACAGAGCAGAATGTTAATTGAATCCATAGCCTCAAATTGAATCTCGCCCCTCGCGAACCGCTGCCATTTGTCGGAAAGCCTTAAACCCGACAATATGCATTGGAAATTCCCAGAAAGAAGCCGAGAAAGAATACCGCTTATATGTTGGCGCTTGAAGTTGGCTCGAATTTCTTTCGTTGTTAACAATAAATGTTATTGGAATTTGCATTGAGCTCAACGAGACAAAGACAGCGCTGACTTCAGTCGACTTTCGTATTCATTGTTAAATGACATGCAAATGTACGAATGACATGGCATTCGCCAAAGGGTTTTGAAAGGGGGGCCAGATCCAAAGGGCAGGTCTCA

GGCAGGTCTCAGGGAAATGTTTCCAGGCTAATTGTGGGTTTTACGCCCTGTACTTCTCCAAATGATCAAGTACGTCATTTAATGGAAGCCACTGACAATTGGAATCGTAAATTATACAGCACAAACTAGATTTGTTTGAGTGCTCTCAATGTAGGCTAATATTAGATTTCTGCGCTGAATTAAAATTATTGTAATACGTATTATAATGCATTTGTACCCAAATTTGACAGACTTAAGCAGTTCTCTAACATAATTGGCATCATTGGCAAAGAGAAATAATATTAAATTGGCAGCATTGCCAGAAAAAACTCTTCTCCTAAATTTTGCTTGATTGAATGTTGTAGTTGAGAATGTTGTAAAATAGTGTTAGTATTGTAACACACGACATTTTTCAAATATTTAAATGAAAATCACATGGTAATTAGCAATTTTGGGTGGCCTTCTTTCCTCCCCAAGCCAAAGCCATATAATTTCAGCCAGCTACTTGCGATTTCCCCCATGACCAACAACAACAGCCCCATATGTGCAGTGCATTAATGCAGATTTCTTGGCAATTGTTTTTGCATACTTTGTTTTTTCCTCACTCACTTCAATTTCAATTGGCGTGCTAATAACTCATTTAGTTCGCAACAAAAAAACAAAAAACGAACAGCGGGCCACAAAAAATGTAGCTACAAACATGGCACACCAACAATGGATTGGATGGCTAACCAAGATCGCCCCCACTTCCCTTTCCATCAATTGCGAATATATCGCATCTCATGATGCTGAGAGAATACTCGTACTCAACTATGCCGACTTTATATGAACACTGTGTGCAGTTTTGTTTTAGGCTTTGTAATTATTATAAAAATAAATTGAACTATTGTTGCCTCATTTAGATTGAACAGTGAGGCAGCCACAATGTTG

 

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