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CRIMIC CR00938

Gene Ced-12

CG5336

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:12098202..12098202	33C4	FBti0199695	79366

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGGAACTGGAGTACATACTCATGG	ATATTCTTACCCGGCGGCGTCTCAAT

Images

Construct Design Sequences

sgRNA sequence	GGAAGTTCCTCCCTTATGATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCGACGATGTCTTGACCAA	TGGTTGGAAGTTCCTCCCTT	AACTTCCAACCAACGCCCTT	CGAGAGTCAACGTTTGCAGC

 

Amplicon 5'

Amplicon 3'

CCCGACGATGTCTTGACCAAAAGAGTGCTGGTCATGGACATAATACATCCTGCTCAAAGTAGATCCATGTGCTTTACAAAGGGCTACACCCATTACACCGAGGGCACGGGCTCTGCATACACACCGCTTTCGGAGGACGAATCCCACCGCATCTTCGAGTTGGTCAAGGAAATTGACACAAGTAATCAGGATGCATCGAAGTCGGAGAAGATTTTGCAGCAACGCTTGGACCTGTTGCACCAGGTGAGACTGCGCTATTTCACGCCACGGGAAGTTGCTCGTCTAATGAGTTTTCCGGAGAATTTTGAATTTCCGCCAGAAACAACGAATCGACAAAAGTATCGACTGCTGGGAAATAGTATTAATGTAAAGGTTGTCGGTGAACTTATTAAATTGCTGACGATAAAATAAAACAAAATACAACATATTTGCTTGCTTATTTTCACTTTAATACATTTATTAGCTAGGAAATTACTCTGCGCTGTTATCATTAAAATATTTATTTCAAATCCACATAATTTTCCCGCCCAGTGTTGCCTATGCCACAAAACGCAACGGTTGTGTGCTGTGTGATATTCCATACTACTTGTCAATTGGTCACACTGCACTGAATTAAAAAAAAAAAGAATATAAGTTTACGCAGAAAAACAATAATAATTGCAATTATCGCTAATTGAAAGAGTGCCAAAATGATACCAAAAAAGACGACGGTTAAGGATTCACACATTGTGAAAATAGCCGTGGAGCGAGAGGACCATATAGCGCAGTTGATAAATCTGGATCAAAGGCATCCGCTGGCAAGTGAGTAAATAGCGGATAAAAACAAAGATTTTTCGCTTGCTGAGCAGCAGTTTTAAAGAGAATCTGCTTTAAACATGTAAAACAAAGTTCCAAGAAGTTAATAAACCAGAATTATGTTGAAAGTAGCAAAGGAACCCATCATAAGGGAGGAACTTCCAACCA

AACTTCCAACCAACGCCCTTCGCTATGTTTAAGTGTTATCGTGATGTCAGCGCACTCATTTCCTCTTTCTCATTTATATGCAAATCATACAATTGGTCAGGAAGTTGCTATTTACAGCGTATTAATGCCGCCTTCGCTTGCAGGTAAAATCCAGGAAATTTGCAATGGTTGGTCCATCAGCGATCACCAGAACTATGCACTCCAGTTCTACGAGCCCACTAACCGAAAGTACGTGACCGAAAAGAATCGCAATGAGATCAAGAATGGCTCCGTACTGCAGCTGCAGTATTCGCCATCCAAGACTGCCTGCGATGCCATGGAGGTGCTGCTCAATGGCAGTCCACAGGAGAAGGCGCTGCGTCTAAAGGAGCTCACTTCCCTAAGCACCGATCACACATTTGCCCTGGAGTTCATCAAGGAGAAGGGTCTCGACACACTCATTAAGATGATTGAGGATGGCGGCCAGACCAACGAAGACATTCTGAAATACAGCCTGGCCAGCTTTGTGGAGCTGATGGAGCACGGCACGGTGTCCTGGGAGGTGCCGGAAAACTCATTCGTGGCCCGCAACATTGAGATCGTGCGAAACTTTCAGAAATATCCTACGAACTGTGGTGAGAGCGCCCTGTCCAACTTGGAGAACATTGTTATGTGCAGCAATAAACATGTGCTTGTTGCCGAGGACATCAAGCTGCAGGATATTCTCCGATTGCTTCAGGATGTCAATTCGCCGGTGATGCGACAAAATGCCATAGCCCTGCTGAACGCTCTGTTCGTCAAAGCAGACGAGGGCCGCCGCAGGACCATTGCTCAAACTATTAGTGCCAAGCAGTTCCGCCTGGCCCTTATCGGCAATGGACTGGGCACAGAGATGACCCACCAGCTGTACGTGCTGCAAACGTTGACTCTCG

 

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