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CRIMIC CR00940

Gene Smg5

CG8954

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:13900630..13900608	34E2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGTAGAGCGCAGATCCAACTCACAG	CACTTAACATTTGCTGGGAGGTCGTC

Images

Construct Design Sequences

sgRNA sequence	TGAGTGCGCATACCTCCGCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCGGTGTAGCTGAAGTTCT	CAGGTGAGTGCGCATACCT	GAGGTATGCGCACTCACCTG	GCTACCACTCGAATCCGGAG

 

Amplicon 5'

Amplicon 3'

GCCGGTGTAGCTGAAGTTCTGTTGCTGGCGGTTCTTCTCGTGCAAATTATCGCCGGACAAGTACGTGACGACGCTCTTCTGCAGTTCGCTGACGTGCGGATCGGCATGGTTGGCCACCAAGTGATTGCAGAACTGGGCAATTTGAAGGAAAACTGAGGCGTCACGATCTATAAGTAGCAATAAAAAAAAACAACACAACACCATTAGCTGGCTAATTCTCGTGCGATAAATATTTGCTAAACTCACCCATCTTGCGTGATATCTTCAGCAGGGATAGCGGCTGACTTTCGTTATCGCGCTGGGCACGCAAGTGCCTATTGCCCTGTTTAAACTCCTGCTCCAGCCAACGGATGACATGGCGCACATTCTCGCTGCGTTTCTTCAAGTCATCCAACTCATTGAGCACTAAATGAGAAATGATAATAGATTAGCATGGGGTCTTTCTCCTTGGGTACAGGTTAAGTACATTCTTAATTGTAAATACACTTTACTATAAAAACAATTGGAAAGAAAACAACAGGCAAATCAAATGAGCTACCGTCATATCAGAAGTGTTAAGAATCTAATCTGTTCTACTATAAAAGCTCACTGGATTTCAGGGAATACCACAAAGTAATAAGCTTGTTGATATGAGATTGTATAGATTATTTAAATTTTGAAGTTCTAAAGTCGTCATTCTATGTCATTGTTACTTTCCACTTTGTTATCAACCCACGCGGAGGTATGCGCACTCACCTG

GAGGTATGCGCACTCACCTGCATTTGGAATGAGGACAATGCACTTCTTGGTCCTCACCAGCGACTTGACTATGCCATTGTATTCGGTCAATGCCTTCGAATCGACCACCACAAATGGGGACATCACCACATGAGCGGGACTGTCCCGCAGCTCCTCCTCCAAAGTCTCCACCTCGTTTTGCAGCCACAGCTTGCCCATGAGCTCACACTTCTTCGACTTGGCCTCCGAGCGCTCTTCGTCGGCCCCCGAGCTGGACTGCAAATTCAGTTTCTCGATGACACTGTTTGTGGTTGTGGAGGCGGAGGTTCCGCCGGCGACAGAGGTTCCCGTGGCACTGGTTATAACAGCAGCCGCCTTCTCGGCATTGCGATTCCTTAGGTAACCCTTGCGTGGCATCTTGCGGCACTCCTCCACGGTGTCCTTTTCCTCGTTACTGGTGTAGCTCTTGCGGACGATGCCGTTCTTGCGATCGGAGTAGCGACGTGTGCGTCCTTCGTTCCGCTTTGTTCGGTTGGTCTTCGGAGCGGTACGACGCTCGCGGCGACGTGATCCGCCTCCCTTCTTCTCCTCGCGCTGTTTCTTCTTTTGGGTCTCGGTGAAACGACGCGTCTTGACGCAGAATCTGTAGTTAAACTTCTTCTGCTTGCAAATGAAAAAGCCAAAGTCGACCATTTTGAAAATACGGAGAATGGACTCTTCCTGGGGAGTGACACGCTCCCGGATAATGGTCTCCCAGTCCAGTGTAATCTGGGTGCTCTGGAAGATGTCAAAGTTCTTGAAAGCAAAGTCCTCGGTGAGCGGAACGTTGGCGCGCACATCGAATAACTCGACCAGAGAATCACGCACGTTGGCAGTGGTCAGAAGGTCGCGCTCGAAGTAAACCTTCCTGGTGAAGATGTCGATGTTGCAGTAGTTGAGAAGCTTCATGATCTTGTGCACAAACTCCGGATTCGAGTGGTAGC

 

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