CR00941
CRIMIC CR00941
CG30372
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TAGTTTCTCAGCTATTTGCCGTCCCG |
TCGAGGCAATTTCCCTACCGAAACAC |
Images
Construct Design Sequences
| sgRNA sequence |
CTGACTGAGTGTTCCCGTACGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGAATGCAAACAACACGCCT |
GGGAACACTCAGTCAGCTGG |
TTCTCGGATTCGCTGCTCTT |
ACCTACAGCCCAAGCAATCC |
| Amplicon 5' |
Amplicon 3' |
| GGAATGCAAACAACACGCCTAGACCGAAAACAAAAACCAGCTAACGATATTCGTCAACTTGTTTTGCTCTTTGGACGAGTTGCGCGGCCAGTTAGCTGTTTTGATAACAAAAGATGCTAATTTATAATAAGTCAAAGCCCCAGAAGATGGCTAAAACAGCAGCACTTTGTTGTGGAGCGGCCAAATGAATGGCACAGAGAGAAACTCTTGGACGCACAAAATTAAATTTCATTTTAGACAGTCAGTTTTACAGCTGCTCCAAATATAATAAACACGCAATGGGAGAGACTGGCTAAAAAAAAAAGAGAAATAAAATTCATTTAACTGGCGGGGAATGGAGGTCACTGGGCTGCTTGGGCTACACCAAAAATCCATCAGCAAAATTAAATATTAAATGTTTGACGTGGCGTCCAAGAAGGCGGCCCAGAGTTCCGGGAAAAACTGGGCATGACGTGACCGACCAATAAATCGTCTAATTTTGTTTCGCCGGGCGGGCGCTGCATTAGCCTGGGGAATTGGAGCAATATGTGTATATTGCACAACCGGCCCACTCAAGGGTCTTTTGGATGACAACATGGATGACTTTTCCACTTAGGCGTTCTGTGTTTTCTGTGGACGATATCCCCAGCTGACTGAGTGTTCCC |
TTCTCGGATTCGCTGCTCTTATCACACGCCTTTTTCCGGCCGTAAAATACTCTTTGTGTGCTTAGCTTTATCAGCGGGCGATTTATATCCGTGCACTTACATACATATGAAAGTGGAGGCGACATCACAGCTGCCTTGAGTAAATATAGAAATTACAGCATGCTTATGTGGCAGCCACTCCCACTTCGGTGCCCAGCCCCCAGTTCCCTGCGCCCCTAGCTCCATCTGCTCTTGCACGTTCCGGAATGCACTTTGTATTTCCATTGTACTATTTTTGTGGAATCGTGGAATCGAGGAAAAACCACCTTGCGGGCAGACAGAGAGAGAGCGCGAGCCGGGAGAGAGAGACCGCACTAAAATAACAGGCGGTGGCTCATTTTGGCGCCGCGCCAATTTACAAATCTGTCCGCTGCAGCCCCCCGGAAAATAGTAATCATCACCGTTTGGCAGGGGGCGTCGCCTGCCATGTTAGCTGCAGTATCGCGTGGTCACATAGCCGTGCTCCTGTTTTCACTTTCACCGACATCGATCAGATTCCATTAAATTAGGAACTTTGTTTTGCAAAACCGATTATTATTACAGATTATCCGCAGATGGATCGGAATTTTAATCATTGAAATAAACAAGTAATGACGATAACACATTCCTCACTCTCCGTAGCCTACGAAGATATTAGTTTTTGAACAGTAAACCCAATTTTTCAGCGGTTCAAATGCCTTTAAATTCAGTGACCCCTAGACTGTTTATATGTTTAGCTCAAATATAAAACTATATAGACTGGGATTCCCAGAACACACCTGTAAACCCACCAGGATAGCCCACCTTAAACTAGGGATTGCTTGGGCTGTAGGT |
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