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CRIMIC CR00942

Gene Not3

CG8426

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:5659705..5659705	41F6	FBti0206922	83203

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCCTGGTAACAATCCTGCGGTTCAG	CAGGAACCATTCTCAATGCAGACCAC

Images

Construct Design Sequences

sgRNA sequence	CCTTAACGCGAAATGTGAACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTCGCCAGGTTTTTCCCCA	GTGCCCCTTAACGCGAAATG	CATTTCGCGTTAAGGGGCAC	ATGTAGGGGGTTCGGGTGTA

 

Amplicon 5'

Amplicon 3'

ATTCGCCAGGTTTTTCCCCAAGGATCTGGGGAACATCATGTAGGATCTTGGGTATCAACAGGCCCTTGGGAGTAGAGCTGCACTGTTTTGCTGACGATGTGGCAATTACAGCCGTCGCCAAAACAATTGCAGAGCTCAAGAAAAATGTAACACCACGATTGGAGCTACCATCCACTGGCTCGAGAATGTCGGGCTAGCAATCGCGGCGAACAAGACCGAGACAGTTCTATCAAGCAGCAGAAGAAAGGTGGAGACTATGCCGGTCTCCGTGGGACACAGTACCTGGGTGTTATGCTAGACCACAGAATATCGTTCAAGGACCACGCTAGTTAAACCACTAAGAAGGCAGCAATCACAGCTTCTTCATTGGCTAGACTCATGTTCTGCGTCGATTGTGAGGAAACAGAGCATGTACTAATGCACTGCTCCAGGTTTTCGGCGGAGAGGGAGCAGCTAAAGGCGTTGACAGGTACCCCGTTCAGCCCTAGTGGCTTGTTCGCGGCTATGTGGGCAAACAGCGAGGCATGGGAGTTGGGACACAGTTAAATCATTAATATGATAAAATTAGTCCAATCTGACGAGATGGCCAACAGAGTGGGTGACTAAACCCAAACAGAAGTCCTGTGTGGTGGCGGGAGAAGAATACTACCTCATCGTACCAGGTTTGTCGTAAAAGGCTAAAAGTAGGGGAAAGGGATCGGATCTGTTAAGTTTTTAATCATCATGCTTAAAAAATCAAAAGCTATAAAGGATCTCAATTCCTGTTCACATTTCGCGTTAAGGGGCAC

CATTTCGCGTTAAGGGGCACATGTTAAAAAATGAACTAAAACATGTCTTATTTTATATTAAAAGCAAAAGTTTAAACAACCCACAAATTTTATTATTTCTGTCGACGCCAGCCCACGTCGTGACGAAGCGCACCAGGATAGTGTGCGAAGGCGACTACTATCCATATGTACATGAGAATATGAAAATATGCTTTGGTTGTCCAATAATTTGAAGAAGTATTTGAATCCTTTTTAGAAAATTTTGGAGCTAACATTGCTCACACAAATGAATAACAAAATCTTTGCACACGCCTACGCGGTGCAAGTTGGTTTATCTTAGCAACGCCCACAAACAGCAAACAGTTTTCACGCCTACAGATTATTTAAGATTTTCCTATTTTTCTTGCCAATCTAAAAATTTAACGCTATGACAACAAAATGCTGAAAAATCTCCCTAACACTGCCACTAGATAAGCAACGGGTTCCTGATAATCGCGCAAATTGACTATTGCCTTTTTTTTAAGCTAAGCCAAAATTTCCTAATTTCTATATATGTATAAATTCCGCAAAAATGATGAACTCTTAAATGTTACTAAGCCCAAAAAATAACATGAAAACTAATGCTTTTTTTTTTAAATATAAGCTTCAGAAAACAAAAACACTTCCAGCATACAAGAGTTTTGACTAAATAAATGCATTATCTAAAGTTTACTCGTTAAAAATGTTTTTCTTTGATGCAATAGATAATCGAAAACGTACTACAACAACCATTTATTATGCAGATAGTGACAATAGCAATTCTTGTTTTGAAATGGTTCTTGAGACAGCAAAAAACAAACAAAAATATATGTTCATGATCATATAAATCTATTCCCTATAACATCAGAAAAAAACACATATAACTCTAGCCTTACCATTTTGTATGTGAGGGGAATTTGTTTGAAGATTGGATAATGGTGGCACATGTTTTTGCTGCGCCTGCGTTCCAGCTCCTACGATAGCTGCCACAGTAGGAGTTACTGCTAGTTGCTGTTGCAAAACAGGACCATTTTCCAGGAGTGCTAAAATAAAGACAATTATTTAAGTAAAGGCAATAAATATGTACACCCGAACCCCCTACAT

 

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