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CRIMIC CR00943

Gene CG13124

CG13124

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:9905485..9905505	30E1	FBti0206849	83204

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTATGTATCTTTCGCGGAGACTCAC	GGGCAATGATGTTCAGACACAGTCGT

Images

Construct Design Sequences

sgRNA sequence	AGAATAGAGCATCTTAAGCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTTGCCCAAACCTAGTCC	TGCTGCCAGCTTATCAGTTC	CTGGGCTGGCTGAAGTACATAT	AAGTAGTTTGCGCCTCCCTC

 

Amplicon 5'

Amplicon 3'

TCGTTGCCCAAACCTAGTCCCAATCCGGCCATTCCACGAGTAAGGGCATCCGCCGACGACAACGACTTGGAGCGTTGCAGTGCATTTCCATTCATGTAAGGATCTACTCCATTAATATTCGCCTGATAAGGCTGAGCATAGTTGTTGTTTAGTTGACCAAACTGATTATGCGAAGCCACTTTGTGACCTCGGGGCTCAGGTGCAAACTTTACACGATTTGTTGTCTGGAATTTAGGTGTTTATTTATTGAAATATTGTTTTTTTTTACCAATATTTTTAAATTAGAGCTCACGTGAAGTATATTTCCACTGGATGGCGAGTTCATTAGCGGCAGCTGACCGCTGAGGGCACCGGTATTGATTGGATACGTCACCAGGATCGACCGGTGGTTGGCCTTCAAGCCCGGCATGTTGCCGAGGGAGTGTCCGGCCTGGCGGCGATTCACGTTTCCGTACGGACCCAAACCCGGTGCAACTCCCACTCCGGGTCCATTGGTGAGCGGAAGTATTAGCGACGAACTGGAAGAATGTTGTAGACTGTAGTTTAGCTCTGCAAGGAGACGGGAAGAGGGGTAAACAATGGAATTAAAAGACTTTGCATTACCGGTTGCCGAGTGCCTCGGCCGGTGCCCCGGAGCTGGTTGTCATTGTGAACTCCTGGGCATTGACGTTCAGCTTGTTGGCGACACCACCGATAGCACCATTCGGACCATCACCACCGACACCACCGGCTGGAGCGGCTAGCTCTCCGCGCACATCTGAGACAGAGTCAAGTGGGGATTATTGTTGGGGAAGGGTCGGTATACCCAAGAGTGTATGTGATGATGTTGGGGGGTAGGACAACATTGTGTGGAGGGACGGAATGTACAGGGTATGAGACAGAGATATGAAAAGAATCAAAAGAAAAATGTGGTCTCAGTAAGTATTAATTGGAACTGATAAGCTGGCAGCA

CTGGGCTGGCTGAAGTACATATGTGACTATTGACATTTAATATTTACATTTTTAAATATGCTCAATCTCACTTTTTAAGGACTAAGCTGGAAAAAAAACGGAAATAGTCCCTATAAAATATGCCTATAATAAATATCGTTATATGTACATGTATGTTTTTGATTATTCAGTACCTTTCTCGTCCAAATGCATATATCTACACTTGATGTGTTTGTATTTCTAAGCCACCACTAAAATAATACCGATTAAATATCCTCGAAATATAAAAATTCTCTATTATATTGAGTTCTTTAACTTCTTTAGCACCGAGCACCCTGCCAAGCACCCAAGCGAATTAGAGAATTTATAACGAATGACTAAAACAACTCACTGGGAGGCCTGTAGATATCCATGGCTGGTTTGCCCCTCATCTGTCCCGTCTGATTGCCAGGTACGTTGAAAGCCAGACTTCGCCGTTTGTTGATATTCTGAATGAACAAACAATTATATAAATATGGCTGCTAATCGACATCGATATGGTGTACCCACCTGTTGATTTGAGGTGCGCACAAAGCTGCGTTCGCGGGAGATAACGCCGGGCTGAAATTCCGGTGATTTGCGCACCTTCCGCGTATCATTCTGTTGGCCATTCATGCTGGCCTGGGCCAGTAGTTCCACCTTTTCGGCCATCCTGCGATTTTTGCGCGAGTACTGCTTCTGCTCTATTTGGGTAAAACCTAATTGAAGATGAAAGCCTAATCTTTGGTAGATTATCCTCCGGTTAATCGATTCACAACTGGGATTCCGTCGAATAAACGTCTAAATGTGTGGCTTCAGTATTTGAGTAAATTTGTACATATCACAATAGTTGGTTTCCTTTCCTCCTTTATCGAGTTGAGTTCTCTTCTTAATACGATGTTGAGGGATATCTTCCCCTTTTGCAGATTCAGATTGCCGGGTCTGGAATACAATGAAAATTAATTATATACTTCAGCATATCGCATTATATTATTGTCAACATATATCAAGTAAGATACACAAAAACTCTTTAACCCACATGCGAGAGGGAGGCGCAAACTACTT

 

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