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CRIMIC CR00945

Gene Fib

CG9888

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23073950..23073950	59C3	FBti0206923	83206

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGCTCCCAACTAGAACTCTCAAAGC	AATCCTGTCCGCAGCGCAGAACTTTT

Images

Construct Design Sequences

sgRNA sequence	TTGTGCAGGGGTACAGAACATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCCAACCATTTTCCGCAAT	GGTTGTGCAGGGGTACAGAA	CCCCTGCACAACCTCATCAT	GCGTGTTGACGGACTCAATC

 

Amplicon 5'

Amplicon 3'

GCCCAACCATTTTCCGCAATACCCCTCAGAAAAACCACGTTTGTACAAAGATTGACTTTAAAGATCTTCATCTTTCATAGGAACTCCATATTGTTACACTGGCATTTGGTTTTTGTGTATATTTTGTACGTCTGCTACAAAAATAATGTGCATTTTGATTTATCTTTATAACTACGGTTTTATATTTAAAAGAAACTTAATACAACTCGTCTGTATTAGTACAATAGCCTTAAAACTAAACCCAGCAGAAAACGACTTCTTACTGCTTGGGCGGTGGTCGGTAGACGCCAACGACAACGGCGTGGTCTCGCTCGTAGGGCTCCAGCGTGAGCTGCTCTTGGGGCTTCAGCTTGTCCGCCTGCATCTTCTTCACCTCGGCAGCGAATACCGCTTCGGGCTGCGCCGTCGAGTCGATGCAGGAGGCCTTGATCGAGATGACAAAGTGTCCGCCGTTCTTCAGGAAGTGCTGGGCATTCAGCGCCACAATGCGGCCCTGATCCGGCTGGGCAACGTCGGCGAAGATGGTGTCCACCATGCCCACCAGCATGCGGTACTTGTGTGGATGGCGAGCGTCCTCGATGATGGGTATGATGTTGGTGCGCTTCTTGGCCACGTTGATCAGATCGCGACCGGATCGGTGTGAGAACTCCACGGCGTAGACCAGGCCCTCGGGTCCCACCACATCGGACACATGCGAGACTGTCGTTCCGGAGGCGGCGCCCAGGTAGAGAACCTTCGAGCCCGGCGGCATGTGAATCTGCTCGACGCCACCCAGAACGGCGGCAGCCAGCTTGGAGCGGAAGGGATTCCACACGCGGTACTCAATCTTCTCGCCATTGGTCTGCAAAGAAGAACAGATTTAAATCCATTGCTCTTTCTTAAAAATTATAGCAGAAAGCAAGAAGTAAGTAAGTTGAGTGAAATACAGAGTTGTTTGGATTGTTCAGGTAGCTTCAAATCACTTTTCAAGATGGTGGACTTGTTTGGGTTCTGTTTGTTTTGGGATAGAAGGGTAGATAGATAGGAATTCGCCTCAGTTGTGCATTATTCTAAGCAGCCCTTGGAGCTCCGCGACTGCTTCACGTGCACCTGAAGTGCACACAGCCACAGGTGACCGCCAAGGATGCTCATGGCCACATGATGCCATGTTCTGTACCCCTGCACAACC

CCCCTGCACAACCTCATCATAAGCTAGGAATAGGGAAGGTAAGTGAATAAAGATGAGAACAACAAACCTCAACGGAGATGCGCTTCTCGCCGTAGACTTCGGATCCAGGTACAAAGTTCCTGGTGACCAGAGCGTCCTCCTTGCCGCGCGCAATGAACACTCCCTCGTGACGATGCGGCTCGATGGTGACGGTCTTGCCGCCCTTGAAGCCGCCAGCACCACCACCACGTCCTCCTCCGCCGCGACCACCGCCTCCACGGCCGCCGCCACCGCGACCACCGCCGCCGCCACGTCCTCCGAAGGCACCACGGCCGCCGCCGCCACCACCACCGCGACCACCTCCTCCACGGCCACCACCGAATCCTCCACGGCCACCGCGATCGCCGCCGCCACCGCGTCCACGGCCGCCTCCAAATCCACCGCCTCCTCCGCCGCCGCCGCCACCACGCCCACGGAATCCACCTCCTCCTCCTCCGCCGCCGCCGCCACCACCGCGTGGACTGAATCCTGCAATAGAAAAGATCCGCATTTGAATTTCGGTTGACTCAGAGGTTAAGTTGGAGACTATCAAAAGCAAAGGCGACGCCTGCTTCGATTTGTATCGTTAGAGCGTGATTTTACGCACCTGGTTTGCCCATTTTATGTTGCTGTACTTGCACTTTTTAATACAACTTTCGACGAAATGTGATTGAGTCCGTCAACACGC

 

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