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CRIMIC CR00946

Gene Cyt-b5-r

CG13279

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:16790030..16790033	36B1	FBti0206851	83207

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGCTCTTAGAGATAAGACTACCCC	CCGTGATCAAATCATTCGACTGGGAC

Images

Construct Design Sequences

sgRNA sequence	ATTATGCCCCCACTTGTATTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGATCCAGGGTCGGGAAGA	ATGCCCCCACTTGTATTCGG	TATTAGGGCTGCGACACGTG	CCGGCGCCTTATCAAAATGG

 

Amplicon 5'

Amplicon 3'

ATGATCCAGGGTCGGGAAGAGATGGTGGAGCACATGGTCACCAAAGTGGGTGAGCACCAGGAACTGCGACCACTTGAGATCACCGCGGTCGATAATCGTGTCCACCTGGAAGAGACCCCAGTCACGATCCTCACGATTGGCATCACCCTCGTGATAGATTTCCGGATCGTGATGCGCCGCATTCAGACCAATCAAGCAGAAGCTAAAGCTGGCTATCGACGTCATCGCGAGCCACTGGCGCACGCAGATCCAAATGCCTAAAGATCCCCCGGTTCCCAGGTATATGGCAATGGGTATAGTCAGTGGCAGTAGGTCGTGCCAGTAGAGGATGTTGGTGTGGCGCAGCGAATAGAAAATACTGCAATATCAATCAATCAAAAGATCACTGATTAGACTTTGTTTACGATCACCGCCTCGATCACACACTCACCGAGTGCCCATTTGTATGAAGAAGGCGAGGGCATAGGCCACCGGCTCCGTGACCCAGGACACATAACGCATCAACTTGCTCTTGATGTGCGGATTGGGCACCCAGCACAGCAAGGGTTCGAACATCGAGAGTTCCAGATCGAAATACGAATTCGGATAGATGTGGTGGGACAGAGCATGGGACACACGCCAGGCGGCAAAGTTCATCATGCCCAGATTAAAGGCGTACATCTGCCAGTTGTCCCGACGATGAAAGTAGTTGTGGGACACGATCACCGTCCAGCAGAGGGCCACACTCGCCAAGACCAAAGCCAAAAGGCTGTTGTATTTGGCACTGGCAATGCCCAACAGGTAGAGCGACACAACGAGACCCAGGTGAATGAGCTAAAAGAAATGTATATTAATATATTATAAATATAATGATAAATTTTAAAGTTATCATATTAATTAGGGTTTCAGCGTGCAAATAATTAAATAATATAATCACACAGGCCGCCGAATACAAGTGGGGGCAT

TATTAGGGCTGCGACACGTGAAATGTAAGATTAGATGTTTTTAGTTAGTAATAAATTTATTTATGAGCCGGCAGGTTCAACTTCCTATCAATAAGTAACTGATAAGAATTACTTAACTGATATGAAATGAAATCTAAGGCACGCCCATTTTCAATAAGGTATTTTTTTTTGCAGATTAACACTCACATCACTCTTCTTTTTCGGCCGCTTGTCTATGGTCTTTAGTTGCTCGCGGACTCGCTCCTTTAGCGTCTTGTAGAATCCGCCCTCCTCCAAGGTCAGTGTGTAGATTCTGGGTTCAGCCGCATCCCTCACCTTGTATTTGGCGATCATCTTTTCGGGAGCCGTGGTCAGATGATGTGCCTCAAAGGCTTCGGTGATGTCGGTGCCCTTGGTCTCACGAATCCAGAAGGGACCGCCAGGATGCTTGTCAATGAACGAGGTGAAGTCATAGATGCCATCGTTGATGCGCCACAGTCCTTCGGCTCCATCGTCCTGCCGTTTACCCTTTTGCCAGCTGTGTGTGGTGATTAGGGCCGAATTTCGGTATGTTGGAAACTTGGTGGCAATGCCACTCTTTTTCCACTCCTCGATTACCATATTGCCGAGTCCTTTTGATCCTTGACGTCTGTGGCACAAACTTTACCGATCCGAGCACCTTTCCGCTGGGATTCCAACGACTAACTGAAAGGCACCGGCAGTGGTCCGTTTATTTTATGTGTAGCGCAAATCTCTTTTGATTATATAAACCGAAAATCGAGATTTCCAAACAAAACGTTTCCATTTTGATAAGGCGCCGG

 

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