CR00947

CRIMIC CR00947

Gene mmy

CG9535

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6471035..6471013	26D7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AAGAGACACACGTTCGCACACATTCG	AAAGTAGTCGTATGTGGGTTGCACCG

Images

Construct Design Sequences

sgRNA sequence	TCCAATGCGCGAGCACGATATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCGAGACTGCAGTCCCAC	AACGTTTCCAATGCGCGAG	CTCGCGCATTGGAAACGTTA	CAAGCTGTATTTCGACCGCG

Amplicon 5'	Amplicon 3'
TTTCGAGACTGCAGTCCCACATCGTACATTCCCTTGGGATGATCGAAGCCAAGTCGTGTGCCTGCAAAGAGAAAGGACAACATTACGAAGGTTTCCACCAATACAGTTCCCCAATATAATAATTATTGCAGTATGTATATAGATAACAATTCCTGAATGTATTAATCACTTGTTAACGACAAGAACTGCTGCATTTTTTATATCTCACCCGGCTGACCTTAAAAACAAGTTTATTTTCGACCATCGTTTCGCTCTTTAGGTTGTCTTTTGTCTGCGCCTTTTTTAACATATATGTATATAAGATACAAGCTGGCTTGTTTAAGGTCAGTTCCACTACCGGTTTCAAAATGATCTGACTACATTTAATCTACGGCTTATCGGTACAGCAAACGGAACCTCCACTCCTGACCAGAAATTCTCAGAAATCAGAAACCGTTATCTAGGTTCACTACTTATGACTCTTCTCGAATTTGAACGTGCAAGAAATAATGCTATGAGCAATCGGTGTTCAACTCCGTTTCTTGGTTTCTCAAAAGACAATTACGTGATTGGACTCCAGCGTTTGCCCAAGAAACTGGTAAGGAAATGTCAAATGCCGCCCACATTTCAATGTTTCGATTCGAAAGAGCACTGGCCCCGCCTGAAATCAAAATGAGAAAGGCAAGTCTGGGGCTGTAAGAATGCCTTCCACATTGCTGCTGGCCTGGCGTGTTTCTTAGTTTTATACGCTTTTTGTCTTGCAAACGGAATTACAATTGCAATTATTACAATGCAGCTGCATGAGTGGGCAGCTGATTGGGATTGCGTGTATTTGTGAGCACTTTTATATCGAGTTAATTAGCTTTTATAAAGCACACACACTCTGCGAATGCTCAAGTTTCTGTTTTAGTTCGGCTTACACTATGTTTTTTGCTCGCAAAGGCGCAATTTTTTTTTTTGATGGGTAGCCGATTGGGGCATGATAAAAATATCTCAACTTGTACGGTATCTAAGGAAGTATCTTTCAGAGATACAAGCAAATGTGAACTCTGAAACGAACGAACTATGAGTGCAATGGAAAATTTCTTTACTTGTAGCCTTTGATTTGTATATATTTTGATTTTTAAACTGACTACCATATCGTGCTCGCGCATTGGAAACGTT	CTCGCGCATTGGAAACGTTAAAAACCATTATGGTAAGTCTCAAAACGAAAATCGTTCATTTTCGAATAACCTAATCGTTTTTGCTATCAATGATTGACGACTTAAAATCCATTGCTATCTGAAGTTTATATAAACGCCATACAACACTACCAACTGGACCCACAAAGGTCTGTCCCACTTCCAGTGCGTCAAATTGCTCCATAATCACACTATTGACTTAAATATGCTACCAACTAAACACAGGTGTACTATTTCCCTAGCTATTATTCTGTTTTTTCTGTTAAATTAGAGAAACTCCAGCTCACCTTGTCCGCCTGCCATAAGCAGCACAGCGACATGTCCGTTGCTGATCTGGAGGAGACCCTCGTCTCGGTAGGCGTCCAATTTCTCCAGCGGCGCCCTGGCAATGGAGATTAGCTTGCCCTCGGGCAGCGGCTGCAGGCGATCGTCCAGCTTGATGCCATTCTCGTTCATTGAGACCGTGGCGCGGTCGAAATACAGCTTG

Amplicon 5'

Amplicon 3'

TTTCGAGACTGCAGTCCCACATCGTACATTCCCTTGGGATGATCGAAGCCAAGTCGTGTGCCTGCAAAGAGAAAGGACAACATTACGAAGGTTTCCACCAATACAGTTCCCCAATATAATAATTATTGCAGTATGTATATAGATAACAATTCCTGAATGTATTAATCACTTGTTAACGACAAGAACTGCTGCATTTTTTATATCTCACCCGGCTGACCTTAAAAACAAGTTTATTTTCGACCATCGTTTCGCTCTTTAGGTTGTCTTTTGTCTGCGCCTTTTTTAACATATATGTATATAAGATACAAGCTGGCTTGTTTAAGGTCAGTTCCACTACCGGTTTCAAAATGATCTGACTACATTTAATCTACGGCTTATCGGTACAGCAAACGGAACCTCCACTCCTGACCAGAAATTCTCAGAAATCAGAAACCGTTATCTAGGTTCACTACTTATGACTCTTCTCGAATTTGAACGTGCAAGAAATAATGCTATGAGCAATCGGTGTTCAACTCCGTTTCTTGGTTTCTCAAAAGACAATTACGTGATTGGACTCCAGCGTTTGCCCAAGAAACTGGTAAGGAAATGTCAAATGCCGCCCACATTTCAATGTTTCGATTCGAAAGAGCACTGGCCCCGCCTGAAATCAAAATGAGAAAGGCAAGTCTGGGGCTGTAAGAATGCCTTCCACATTGCTGCTGGCCTGGCGTGTTTCTTAGTTTTATACGCTTTTTGTCTTGCAAACGGAATTACAATTGCAATTATTACAATGCAGCTGCATGAGTGGGCAGCTGATTGGGATTGCGTGTATTTGTGAGCACTTTTATATCGAGTTAATTAGCTTTTATAAAGCACACACACTCTGCGAATGCTCAAGTTTCTGTTTTAGTTCGGCTTACACTATGTTTTTTGCTCGCAAAGGCGCAATTTTTTTTTTTGATGGGTAGCCGATTGGGGCATGATAAAAATATCTCAACTTGTACGGTATCTAAGGAAGTATCTTTCAGAGATACAAGCAAATGTGAACTCTGAAACGAACGAACTATGAGTGCAATGGAAAATTTCTTTACTTGTAGCCTTTGATTTGTATATATTTTGATTTTTAAACTGACTACCATATCGTGCTCGCGCATTGGAAACGTT

CTCGCGCATTGGAAACGTTAAAAACCATTATGGTAAGTCTCAAAACGAAAATCGTTCATTTTCGAATAACCTAATCGTTTTTGCTATCAATGATTGACGACTTAAAATCCATTGCTATCTGAAGTTTATATAAACGCCATACAACACTACCAACTGGACCCACAAAGGTCTGTCCCACTTCCAGTGCGTCAAATTGCTCCATAATCACACTATTGACTTAAATATGCTACCAACTAAACACAGGTGTACTATTTCCCTAGCTATTATTCTGTTTTTTCTGTTAAATTAGAGAAACTCCAGCTCACCTTGTCCGCCTGCCATAAGCAGCACAGCGACATGTCCGTTGCTGATCTGGAGGAGACCCTCGTCTCGGTAGGCGTCCAATTTCTCCAGCGGCGCCCTGGCAATGGAGATTAGCTTGCCCTCGGGCAGCGGCTGCAGGCGATCGTCCAGCTTGATGCCATTCTCGTTCATTGAGACCGTGGCGCGGTCGAAATACAGCTTG

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