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CRIMIC CR00948

Gene TppII

CG3991

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:13156705..13156721	49F4	FBti0206852	83208

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTTTTTCGCACTCTCTATACCGG	TGCACCACCGTGACAGGAATCTCAAA

Images

Construct Design Sequences

sgRNA sequence	TGGAACGTGTCGAAGACAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGAGTCCCTTACACAGCGC	AGCCGACACACACCTAACTG	CAAGAGGATTGGACTGTGTGA	AACTGCCCTGGGACAAGAAG

 

Amplicon 5'

Amplicon 3'

AAGAGTCCCTTACACAGCGCTGTGAACGCCTGGCTGGAGTCCAGTGGGATCGACGCGCACGGCAATGGAGCGGGTGCCATAGCTGAGATCCAAGAAAGCTCCACACTGCACCCACGGCTGCGAGGCAATCAGATTTAGCCGTACATTGAAGTTAAACTTGTCCTTGGGATCTGTAAAATTGGAACGGTAAGAGCTGGAATTGGGTCTCTATAGTCTCTACTCACCCGCCTCCTTGTCATTGTAGAAGATGGGCTCTATATAAACGTTGTAATCGATGGAGTTACGCTGCACGCCCTGACGCAAATGAATGCCCTTGTCTGCGTTGTTGCCCACGCGCACGGAGAACCTATAAGATTAAAACGAAAGAAATTTATTATTTGGTTGGAATTTTACAAGATTGCTTTGACAATTGTTTACTATTCGGTCATCTTAACGATATACCTACTAAATTATAAAACAAGTTAGCCCTTACTCAGCTAGTGTGAATGCGAACGCGAAATTTAATCGTTTTTTGTTTAGAGATCCTGAGATCTGGCGTTCCACAGCACTCACCTGAGCATATTATCCTTGGACTGGCGGTGCTCCGTCAAATGCTCGAATGCCTTCTCGACATTGAGCAAGCCATGGCCCTGAGCAAAGGGATCTACATAGCCCAGTTTGGTGGCAGTGACGCTGATCGCTCGCTTAATACTGTACGGCGAATACTCGATGTTCTGTTGCTTCAGACCGGAGATGAGCAGCGCCACTGCGCCGGCGACGTGAGGTGCCGCCATGCTGGTACCGTTCATCAGCTGGGACTTGCTCATAGTAAACTGTGGCACGGACGCAATGGCTCCGCCCGGAGCGCATACGGTAACGCCTTGACCTCCGTCGATGCAGGGATCTCGCGATGTCCAGGTGTACACGTTCCCGGGCAGCTTTTCTCGCATCGCATACTCGGCCTCCATCATTTGGGGTGATACGTACGCGCCCACGCCGATCAAACTGGGCTGGCTGATGTCCGGCGGAGTTCCCACAGTACAAAGTGCCGGACCATGGTTGCCGGCCGATGCCACCCACACCACGCCATACTTGTTGACAACCTCGTTCATGAGCTCCCCAATGCGGCTGCAAGAGATTAGAGATATAGATTAGGTATTTTTTCATGAATTTTGATGGTTATCAGTTAGGTGTGTGTCGGCT

CAAGAGGATTGGACTGTGTGAATATTTGGATTTCTCATGAAATTTACCTAGACGCACACATCTTTCTTCTGCGACGCATTTCATCCGACCCAGACCCTGACCCAGACACACTAAAAGACATCACCAACTTTGGGATTAGTTCCGCCGGCCTTTCGCACTTGCTTACCCGGAATTTGACCAATTGGCATGCTCGCCATAGCTCATGTTGATCACATCGATGCGTCTGCCATCGCGACATAGTTCCATCACCTTAGTCATGGCACGCACTAGTGCCGTGCCGGTCTCCATAGACCCAAGTCGACCATCACCGATGGTCATAGAAACGATCTTAGCATTCGGCGCCACGCCATCCACGTCCCGGGAGCTATGGTTGCCGCTGGCAATAGACGATACGTGGGTGCCGTGGGGTGAGCTCATGCCGACCACCTCCAGTACGTTGCCCTCGTCGTGGACGTTTACCGATATGGAAAGAAAGTCGTCCACATTGCGGGTCTCGTGCGTGCGGGAATACTCGCCAATGCGCAGAGCCTGATCCAGATCGCCCTGCTCCGTGGTGTCGACGATTGTCAGCCATCCGTCGGCCGTGGGAAAAAGGATGCAATCGTAGGAGGTCTTAATGTCGCCGTACACCTTCTCGTAGCTATTCAACATCTCTAGCTCAAAGTCTAGATTCTCTTTCAATATCTTCTTGTCCCAGGGCAGTT

 

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