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CRIMIC CR00952

Gene CG3511

CG3511

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24573704..24573714	60D5	FBti0206853	83211

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CAGCCAAGGTCTTCGATGTTGTCAAC	TTCGAATCTGGAGCAGATTCAGTGCC

Images

Construct Design Sequences

sgRNA sequence	GGTTAGTTAAGAAAGGTTAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTCCAGCTCGGATTCAGA	AGAAAGGTTAATGGGATAAGCCA	CAAAACATGTTTCTATTGCACGC	TCACCGCTGCATTCATCCTT

 

Amplicon 5'

Amplicon 3'

TCCTCCAGCTCGGATTCAGAGAGTAGCCGCATACACATCTACGACGGGCAAGGAGGCGGAGAAGCCCTTCACACCTTGGAGAAACTTCACTCGGCGCCCGTGGTGGCGATGTGCTTGAATGTGGCCATGGAGACCGTCATTTCTGTCGATCGCAATGGCATTCTCGAATACTGGCAAAACTCGAAGTACGACTACAAGTTTCCACAGCGTCTGGTGAATTTTGACTCCAAGCTAGACACAAGTATAGATCGATGATTAAGCTGAGTGAACCTAGACTCATCTAAATATTAACCTTTTAGGCCTCTTTGAGTTTGCCAAACAGAAGACCCAAGTCACGGGTTTAGCCGCCACGCCGGATGGAAAGCGTTTTGCCGCCATTTCCACAGATCGTAAGGTCCGCGTCTTTCAGTTCAACACGGGCAAGTTGATCCGGGTATTCGATGAAGCTCTATCTACATACACCCAAATGCAGCAGACGAAGCACGCCCTGCCCAACATGGAGTTTGGCAGAAGGTAAACTAGAATAAAATAGTTATTATTTATTGGTTTAAAGATATGAATTCCAGAATGGCGGCTGAGCGGGATCTGGAGAAGACTGCCCAGAATGCAACCCTTAATATTTTGTTCGACAGCACTGGCAACTTCTTGCTCTACCCAACGATGTTGGGCATCAAGGTGATCAATGTGGTAACGAATCGCTGCGTCACAATTCTAGGAAAAACCGACAACATACGGCCCCTCCAGGTGGCCTTGTTTCAAGGAAGAATTAAGCGCCAAAAAGCTGCTATAACAATGGAGCAAGAGGCCAGTGAAAACCCAGCACTCCAGAATATCCTTAACGATCCAACAGCCTTTTGCACTGCTTACAAGTTAGTTCATACCGTTTTGAATTATATCTATGCTGTATTATAATGTCCATTCTACTACTCTTATTAGAAAAAGTCGCTTCTATTTGTACAGCCGAAGACTGCCCTCGGATCTGCAGGATGTCGACCGCGATATTTTCAACGAGAAGCCCTCGAAGGAGGACATTATCGCCGTGCCGGAAGCTTCAGGTATTTTGGTTTGGCTTATCCCATTAACCTTTCT

CAAAACATGTTTCTATTGCACGCCAACAATATTTTAAGACGAAGGTTATTCCTATCAGATATTTCAAGAACAATTCATTACAAAATGTTCCTTCTATTCTAGTCGTGCAACGCATCTACGAGAACGTGGTGCTCCACACCACGAAAGGCGATATCCACATGAGGCTGTTCTTCAAGGAGGTTCCAAAGACGGTTGAGAACTTCTGTGTTCATGCGAAAAATGGGTATTATATATACGTCAACTGTTGAGAACTGTCCCATTGCAATCACATTATTTTGTCAGGTACTACAATGGACACATATTCCATCGCGTGATCAAGGGCTTCATGGTGCAGACTGGCGATCCCACGGGAACGGGCACGGGCGGCAAATCCATTTGGGGAAGCGATTTCAAAGACGAGTTCGTGCCGAGCCTGAAACACGATCGGCCCTACACGGTCAGCATGGCTAACGCGGGTCCAAACACGAATGGAAGCCAGTTCTTTATCACAGTCCTGCCCACGGTAAGGATGAATGCAGCGGTGA

 

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