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CRIMIC CR00953

Gene CG30122

CG30122

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18635975..18635975	55E3	FBti0206926	83212

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGAATTTCTCTGACACCGAGCAGC	GCGACGTTTCAATTCATCTTCGCTGG

Images

Construct Design Sequences

sgRNA sequence	ACTGGGGCTCTGATCCCCTCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGGACAGTCGTCGAACTTG	CAACACACTGGGGCTCTGAT	ATCAGAGCCCCAGTGTGTTG	AGAAGATGAGGATGGCCCCT

 

Amplicon 5'

Amplicon 3'

TGGGACAGTCGTCGAACTTGAGAAACACGTTGATGATACGGCTCGGTTGGAGACCTCGGTCTACCGGGATCGAACCCAACTCGCATTGAGCTCGCTTGCTTTCAGCGCTTATAATCTAACACATACCTGTTGCACGGCGGGACACCCACTTCCCGCCCACACTCCGCCGGCAAGGGAGCGGGGCCTTACGGAAGCGGTGCGCGCACACCAACATAATACCTAGGGGGCGGCGACGGGTTAATGTTAACCCGGCCACATCTAACAACAGTTGACTTAATACTTTGCATAAGGATAACAGAATGTACTATGGGGGAAAAGTAGTCGCGGTTGGCTGTGCTGCTGGCTGTAGGATGAAGCATCGCATAGCTTTCTGTAGATACACATACATATATATGAGAATAGTTGGCATTTAGAGAGAGAGATAGAGTAGAGATAGTAGGTACTAATACGTATATGTGATTAGATTGTAGGCGAACGACTCGTCGATCGGATTTGTGTTTGTCTACTTACAGTGGCATCCATGCCGCTCGCTCCTCCTCCTCCTCCTTCTTCCATACTTTACTTCACTCCATTCCATTACACTCACTCATCTTTCTCCAATGCCATTTCCATATCCATATCCACTTCCATCCCCACTTTTCTTACAATTCCCTCTTAACTGCCCCGTCAACTGTTGCGCCTGCACGTAAAACTTAAGCTAATGGGAAGTCCACAGATACTTACTGCCTCAAACTTAAGCACTCTAATTATCGGATCGACTTACAATTAGGACATACCTGCACGTTGGTGCCCCTTCTAGGGGCACTTGGGCACTCTATTAAGTTAGAGCGATGAGCAAAAAAAAAATGCATACTCAAATGTACAATTAAAAAATATATAGCGCAACATTGCTGTGCCCCGCTGGTGGAACCCCTCAATCCCGAGGGGATCAGAGCCCCAGTGTGTTG

ATCAGAGCCCCAGTGTGTTGGGTGGGCGCCAAAGTCATCATCAACGTATAATTCTCGCTTGTTTCGTTTTGTGTTTGGTTATCGTTCGTTCATAGTGTAATCTTGTGTGTTTGTGGGCTGGTGTTTTGTTTCGGGTGTATGTAGGTCATTTGTTAATGGTTTACCTGATCAAGTATGAAATTTCGACGCCGCTTCATGGCAATATCTTCCAGAGCCGCCAAGCTATTTAAGCATATCTCGTACACCTTGTCCCATCGACCTTTGTGCACAGTCTTGCGAGAGGCGCCATCGATCTGCAAATATATTGGTCAGTACTGAATATCTTCAGCGCCTAGCTATGTACATTTATTAACCAACTTACTGTCATCTTTGAAATGAATGCGTCGGGTCCAATCAACTCGTAACGCTTGTCAGCATTCTCGGCCACATGCTTATGGGCCCAATGGGTCTTTCCGGCTCCTGGCAAGCCAACCAGTAAAATGACTTCGCACTCCTTGCGAGATCCGACGCGTTGTGGACCGGAAACCAGCTTTTCGACAGGTACTAAGCCGATCAATACATAGCCTTCCAGCAGGGCAGCTGTATCCCTTGGCTCGGGCACGACATCCTCGTACTCATCTTCGGTCGTCTGCGACCTGTCCTTTTCCTTTTCGGATTCAGCCTTTTCCGAATCTCCATCGGTTTTGGGTCGCTTGTTCGGCGAGGGGCCATCCTCATCTTCT

 

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