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CRIMIC CR00954

Gene Sec31

CG8266

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8895866..8895873	44F3		86334

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGATCTGCGTAAGAGTCAGCAGATCA	GGTGTAGTCCAGGTTGACAGCGTTAT

Images

Construct Design Sequences

sgRNA sequence	GTTGGCCTCGATTAAGAGCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCCAACAGCTAACCACCGA	GAGCCTGGCATGTAAGCTCT	GAGGCCAACAAACTCAAAGC	AAGCGGAGATAACGTGCGAT

 

Amplicon 5'

Amplicon 3'

GTCCAACAGCTAACCACCGAATCCGCCCTTGTGGACCGGGCTAATGCCCTGGAACGCTCTCTGGTCGATGCCAATTATTCGGATTACTGTCGGCAGCGAGCAGACGAGACACCGGATCAGCATGGCCGCTACATCTGGTACTTTATTAAGGCGAACTTCGAGCTTAATCCCAAGGAGGAAATGCTGAACCTTTTGGGTAGGATCTCTCTTTGCGTACGCGGCTACTTAAGCTAAGATTTTGTCTTACTTTTAAGGCTACAACAAAGACGACATCGATAGCAAGTTCAACAAGTTTATCAAGGAAACGGATGGCAACTCGCATTCGGATGTGGATACTCTGACGACCCGCATCTCGACACTGACCCATGTAAGTGCTCATTAGACATTGTCTTATCAGTCGAAAAAGAAAAACAAGCAGCATTGCGCACACTTTCGATCTTCCACGTATGCTCATCTTGCTCTGTATATTTCTATCCGATAACATAACTCATGCTATGCCATTGCCGTAGAGCGACTCTTCTGAGGTCGAGTGTGATCAGAGTACTAACTATAGCACCGATAATTCGGTAAGAGCTTACATGCCAGGCTC

GAGGCCAACAAACTCAAAGCAGCTTTTTCTGGGAACCATTTGAGGTGTATTCTTATACAAATGTTATTTCTTACCCAGCAGACGCAAGATAACAAGTTTGATGGCAATGCAATCGACTCACAAAATCACAACGACTTTGCCACTCCTCCGAGGCCGCAATTCAAGGTTCCCAGCGGAGACCGTAAGTAATACATGTTGTATAGTGCTCACTTAGTTTGGTAACTTTTTATCTAAACATTAATAACCTAAAAGCTTTTCTTTTGCGCAAAATAGTTCTAACTTTGCCAGCTAGTAAGCACTTGTTAAATGATCGGTTTTCTACACTTTACAGACTCGGATAGCCTGATTGCCGAGGCAATACTCACTGGCAATGTGGACGCTGCCGTGGAGCTCTGTCTGGAAGCACAGCGCATTCCTGAAGCCCTGATTATCGCCTCAACGGCTGGCATTGAGACTCTGAACCGCACGCAAACGCGCTACCTGCTGCAGCAGAAGAACGAGCTATCGCACGTTATCTCCGCTT

 

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