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CRIMIC CR00958

Gene NAT1

CG3845

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:12920876..12920895	49E1	FBti0206855	83215

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACAACACCAACAGCCTAGAGCGAA	TCGTTTACACGGTCCAAGACCTCCAT

Images

Construct Design Sequences

sgRNA sequence	GTAAGTATTGTGAACGTCATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACAAGCTAACGCCCGAGAA	ACGTTCACAATACTTACGGCG	ACTTTTGTCTGGTCGGGTGT	TTTCCGTCACCTCTGCATCC

 

Amplicon 5'

Amplicon 3'

AACAAGCTAACGCCCGAGAAGTTCCAGGAACTGAGCGATGAACTGTTGAAGCTGGATCTTAACTCTATTGCTATTTTGAAAGGCGTGATTTTGCTGATATTCGACAAGGCCTTAGACGAGCCGAAGTACTCGTCTATGTATGCGCAGCTGTGCAAGCGACTTTCCGAGGAAGCCCCGTCATTCGACAAGGAACCAAGCAATTCGTCCACCTTCTTACGCCTGCTGATCGCGGTCTGCCGCGACAAGTTCAACAACCGACTCAAGCGCGACGAGAACGACAACAGACCTCCGCCTGAAAATGAGGCCGACGAGGAGGAGCGCCGCCATTTGGCCAAACAGCGCATGCTCGGCAACGTCAAATTCATCGGAGAGCTCAACAAGCTGGACATGCTGTCGAAGAACGTGCTGCATCAGTGCATCATGGAGCTATTTGACAAGAAGAAGAAGCGTACGGCCGGTACGCAGGAAATGTGCGAGGACATGGAATGCCTGGCTCAACTGCTCAAGACTTGCGGCAAGAATCTGGATTCAGAACAGGGCAAAGAGCTGATGAACCAGTACTTCGAGAAGCTTGAACGTCGGTCAAAATCATCTGAATATCCACCGCGGATACGTTTTATGCTAAAGGACGTAATTGAATTGCGCCAGAACAATTGGGTGCCGCGCAAATTGGGCACCACCGAAGGCCCCGTCCCCATAAAGCAAATACGCTCGGATGACGACTCAATCATACGCACTCCGTTCCCCAATCGCAATCGTGATATGCGCAACAATGATCGTGACTCGGACAGCTGGATGAATCGTTTCCATCTGAATATCCAGCCTGGCGGCTATAATGACATGTTTAGCGGACTGAGTGTTACGGGAGCTTCTCCGATTGTCTCGCCGTAAGTATTGTGAACGT

ACTTTTGTCTGGTCGGGTGTATAGTTTGAATAGTAGTCACATTATATTAAATTCTTTGAAAGTACAAAACCTAATTTTAAACAATTTCAGGTTCGCTACGAGCAATCGTGACCGAGACCGCGACAATCGCCAGTACAACAATCGAGGCGATCGCAACAGGGATCGCGATCAGGGCGGTAGCGGCGGAGCTAACTATGGCAATCGCTACAACAAGCACAACCAGAATGGAGGAGGCAGTGGCGGAGGATCCTCCAACAACCGGGACCGGGATGATTCGCGTAGGAATAACGATCGTGACAGGGATCGCAATGACGGACAGTATGGGGGCAATCAGCTGCTGGGTAGTAAGGAATTGGCACCGAGGTTCAAACGCAACTTGATCACCACGAACCAGGATGCGGTGGAGAACTTGCAAATGCGCCCGGCAGCCAACTCCTTGCTCTTCCGAGCGGCCTCCCAGAACCAAAAGTTTCCGGCTACGTTGCCCATGTCAACACCGCCCAATAGCAGCGGCAGCAACAACCAGGGCAGCTCGCAGGGCCAGCTCTCGAACTCCCACTACCCTATGCTCGGCACGCCCACATCCCATCTGATCAATCCAACGCGTCCCTCGTCAACACCATCTGCTGAGTATGCTGATAACCGAACTCTTTTGGGAGTTCAACAGGAGAGGGGATTGAAGGCCACCTCTTCTTCGCCGAACTTTTCATCAGGAGCGGAGAAGTTGGATTTAGACGGTCAGGCGGGCAGAAAGGGATCGCACGACGGCAAAGATCAGGGCAAGAATGGCTCCGTGGAGAGACCAAGCACTCCGGCCGGCGGTATTGGCTCGGCCAAGCAACAAAAGAAAGATAAGGGTCCCAACAAGGAGGAGCTGTCTAAGAAGGCCAGTCAGTACTTCAAGGATAGGTTCTACTACGATGAGGAGGAGGATGCAGAGGTGACGGAAA

 

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