CR00959

CRIMIC CR00959

Gene CG18659

CG18659

Information

Donor Location Cytology FBti Stock number
pM37-p22R:9131404..913143245A10 FBti020685683216

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK ATTGTGTACTATACGCAGCTCTCGCC TGCTTGTGTAGTCGTAGTTCCCGAGA

Images

CR00959-1.jpgCR00959-2.jpg

Construct Design Sequences

sgRNA sequence AGCGCCGCGATAGCGACCAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GAATTTGCCGTACGGACAGC CTCACTCCCGCGACTCATC CAACGCCCACTGCTGCTG GAGAGGAACCTTGGGGCAAG
 
Amplicon 5' Amplicon 3'
GAATTTGCCGTACGGACAGCTGGCCAGATAAGCGAATGGGATGAGTCTGTCGGGGGCAGTCGTATGACTAAGCAGGTGGCGAAGAAGCCAATTGATACAGTTCCCGGTGCTCCAGGAAATGGTAAGCGAATGGCGTCGAACAATGCCCATTGAATAATACAGTTCATTACGCGAACTCGCTGTGTAAACAAACACCGAAAGCGAAACAGCAACAGCAACAGAAACGGCACAACAGGTTATTCGTTTCATGGACAGGCTATCTGTTGTTAGTTGGTGGAAGTGGTCGGGTCGGATTGGTGACTCATCGCCGCCGGAACCCCTGCTTTCCGTCTGGCTATGGACGACGATCGTTGGTCTTGCGTTTGCGTCTGCTACTTGCTGCTGCAGATTATTTATAAAACGGACTACCCTCTGACTAATGCCGCTTCCGACCATATAAAGTAGAGATTTCGGCGATCAGCATCGCCAGTGCGTCTGATTCTATGGGATTGGAAAGTTCCAAAGACTTTTTATTTCAATGTAGTCAGATCGAGCTGGTTATGTTTTACGATTTGCAATATCTGCCATAAACATTTTTCAGTAATAAATGGCTGACGTAGTTTGTTTTTTTATCTAGAAAATCGTACATAAACTGGGATGTGGCAGCCATATGGGTTTTTCGAGTTTTAATGAACCTGTCCTACACAAAAGTGAATGTGTGTATCTTCTATCCTGTGAATCAGGTCAAATGGCTATATTCCACAGCTGTCATACATAAAACCTATTGGGGCATCAGCTTGTAATCCCCGCCCGCATACGCACCTTGTTGTGTCGCAGGGAAAGGCAAACGATGGGATGCCGGTGAGCACCTCCTGGTCGCGGAATCCCTCGGGGAAGCTCTCCACGATCACGCCGGCGGGAACAGCTGGCCCGCCGCTCCTGGAACTCGTGCCCCTCTCTAATCCCGGCGTCGGCGTGACCTCGCACCAGAACTCGAAGAGCTTCTTCACGTCATTTCTGGGGGGAGGAGCGGGAGGAATGGGCGATGAGTCGCGGGAGTGAG CAACGCCCACTGCTGCTGCGAACTGGCACTGGGCGGCATGGGCATACTCACTTGATACGCGATCCCATTTCGAGGAGGTCTAGCACATGTTTCTGTGTCGGTCAGTGGGGGGTGTTCGTTTGACTGGTGTGTCCAATCCGGACTGCTAATGCTTCTAATACTGATTACGCTGACACAATACGGTCGTTTGGGCGACCACTTTCCTCCCGCTGCTCTTTGTCGCCTCAGATTCGCGACGTCATTGGTTCGATTTCGACGATCACGGGGGTGGTTTTGGCACCTGCTTGCTGCGGACTTGGGGCGGGGACCACTGCTCACTAATTTTTTGGCATTTTCGGGCCACAACAATAGATATTTCACTTGGAACTCGCAGTTTTATGCAAGCAAGCACAACAAAAACACTTTTACAACAAAAATCGCGAACAGTGTAACCGTCGGTGGACCGTTCAAAAACGTCACTGAGCCACGAATAAATAACTACGGGAATTTTTTGGAATATACCAAAAGTTGTGCTTGCTCAAATATTTGAAAAGCTCATTTATTATAGTCTTTTGACACTACTGTTTTTTTATATCGATAATTTTTGAAGCTTACATTTTAAGGTCTAACAACGATATATAATATTACCTATAGATAACAAAAATATTAATTTCTTGGAACATTTTTAAAAAATTTTTAAAATAAAAGGTAGATAGACATATTGTTATAGTTAAATATTTTTAATGTTTACATCATAAATAGGCATGTTGTGATGATGTGTTATCGTTTCGAGGCCCTCACCGAATTCTAATGATTCTTTATGTAGCCAAATATAATATTACTAAAATGAATAAATAAGTTATTACATAAATAATTTAAAGGTTTTTATTTTTGTATATACCTATTTTATCTTATTCATAGTTGCACCAATCGTGTTCTACAAAACTATCGCATAACACTGGGATCAACAATATTGTTGCTCGCTGAACGTGTTCATTTTTATATCACTGAGGAACGCGTCCATTAGCCAAGTTCCGCACCACACGGGCGCCTTTTTAAATTAGTCTGGTCTCACTGCAGTTGCACCGTTAGCGTCCCGCGATTGTGTTTATATTTTGGTAGTGTGCACCCTTCTAGGTTTTTCCCGAGTTTCTTTGGATCCCCAACGCTTGCCCCAAGGTTCCTCTC
 
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