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CRIMIC CR00960

Gene Dcp-1

CG5370

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:23551887..23551896	59E3	FBti0206857	83217

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATATCCTCCAGCATGCTCTGCACATC	AAAGTCTAGGGCTACGCGCTGGTTAA

Images

Construct Design Sequences

sgRNA sequence	GATGGGAGAGGGAGAGAGCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGACGTCCTCGATGATCACC	CACATGGACTCACCTCGCTG	TCTCCCTCTCCCATCTCTCT	CTGGCAGGCTTGGATGAAGA

 

Amplicon 5'

Amplicon 3'

GGACGTCCTCGATGATCACCTGACTACTGGATTTGGCCATTGTGTTCAGCAGCTTTTTGGGCGTTTCGCTTGGCTCGGCTGTCTTTGCTGGCACGACCAACAACTTCTTGGAAGCCACCATCGTCATGGGCGCACGGGGCTTCTCCAGCGGCGATGGCCAGTTACCCGTGAGCGGATACTGGCGCAGGAGGGTCTCCGCCCGCTTGCACATCTGCTTGAAGCGGTAGCAGTGCTCGAAGAGGAGGCGGCACTCCAGACAGATGTGCCCCGGCATTCCGTCGCCGGCGTAGACCTGTTTTTAATGTTAGATTAGATAAATGTGTGAGTGACTAGGAAGCTACATTTATTCGGGGAAGTCCGTGCTTAAGCCAAATAATAAGGTAATATTCGTGGAACACCCCTATATGTTTTTCGCTACATAATAAATTAAACGCCATAAATGTCTCTTTCCCTTAAAATGAACAATATAATGTTAGAGTTTAAATTTAAACAAAGGCGCCGAGGACCAGAACACCCTCTACAAATTAATCGATGGCCACCAATCGATGATTAGTGCTATCGACGAGCGGCCATCGCCGCTTGGCGGCAAGTGCATACACACGACTACATGCAATCGCAGTTTGTTTTCGCCGCTCAGAGCACGCTTAACCTCCGTTAATTGATTGATTAAACACGTCCGACTGTGAGCCATAAAAGGACATTCGCTCGATCGAAAGCCGACAAAAGCTGGCTGAGAAGCGCAAAACAGCTGCGCTGCGCAAAGTTTGGCCAACGCAGAGCAATAGAAAACAGAAGCAGTGTCAGAATAAAACAAGAATCAGACCAAAAAGGGCGCAACAACTGCGGAGAGAGAAAAGGAAGGAAAGAGCAGCGCGTGAGCGAGGGAGGAGCAGGAGGGAGCGAACAAAAGGCAAGAGGAAACACAAGGAGAAACGCAAACAATCAAAACAAGAACTTAAGCAAGAAAAGGGGCGCAAGATGACCGACGAGTGCGTAACCAGAAACTACGGAGTAGGCATTCGCAGCCCAAATGGATCGGAAAATCGGGGCAGCTTTATAATGGCTGATAATACCGATGCCAAGGGCTGTACGCCGGAGTCTCTTGTTGTTGGTGGTGCAACTGCAGCGAGGTGAGTCCATGTG

TCTCCCTCTCCCATCTCTCTCTTTATGTTTCTGTGTTTTGTTATCAATGCAAGTACTGGCATAACTGCTATATCCTAGTTTCAAATGCAGCATATAAAGCTCTTTTTTTTTTATTTATTCCATTTTCATTCCACAATCTATTATTTTTATATTATTTATACTGTCTATTGCCAACATTTAAAATCAATGTGTCAATCTGTCTTTATAATGTTGTTAAAATGGCTGAAGTCACAAAGTTTGAAAGCACTTGACTTTAATTCTATTGTTCTATTTAGTCTTATTCACAAATATATTTGAAAATAATTACATAAATAAATACCCAATCATCGATTATTTCGCCGATTACCTTATCTTATCATGCTGCTTCCCAAATGGCTATTATAATTCTGATTTCTTTTTCCCCAGCCCCTTGCCGGCCAACAAGTTTGTGGCTCGAATGCCCGTGGAACGATATGCAAGCGAATACAACATGAGTCACAAGCACCGCGGAGTTGCGCTGATCTTCAACCACGAATTCTTCGACATACCCTCGCTGAAGAGCCGCACCGGAACAAATGTCGATGCCCAAGAGCTGAAAAAGGCCTTTGAAAACCTGGGATTCGCGGTGTCCGTGCACAAGGACTGCAAGTTGAGGGACATCCTGAAGCACGTGGGGAAGGCCGCCGAGTTGGACCACACGGACAACGACTGCCTTGCGGTGGCCATACTCTCGCACGGGGAGCACGGCTACCTGTACGCCAAGGATACGCAGTACAAGCTGGACAACATCTGGCACTACTTCACTGCCACCTTCTGCCCCTCGCTGGCGGGCAAGCCGAAGCTGTTCTTCATCCAAGCCTGCCAG

 

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