• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00961

Gene wbl

CG7225

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:19250197..19250213	56C4	FBti0206858	83218

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTTTCCGTCCACTGGCAGGTGTTT	AGGCCTATTCCAGGACCAAGACTAGT

Images

Construct Design Sequences

sgRNA sequence	CTGGATTGCAGCCTAAAGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAGTGAGTTGGCCAAAGCA	TGCAATCCAGGATTCCAAATGA	GCCCTTCTAAAGACTCTGTGCT	TTTGCAGGGTACGGTGGTAC

 

Amplicon 5'

Amplicon 3'

TGAGTGAGTTGGCCAAAGCAGCCAAACAATTTCAGCTGAGCGAAGCGTAAACTGGCCCAAAACTCGAACTCGAATACAACTTTACCGCTCTTCTCGCGCTCGTTTTTAGCCAGTTTGCGCTGACAAACGGCAAGAGTAGGGTCAAGTTCAGAAGTCTCTGGTTTTTGCCACGAAGATGATGCATATTTTGGTGACTCTGCTCCTGGTCGCTATCCACTCGATACCCACCACCTGGGCCGTCACCTGCACGGGCTGCGTGGATCTGGATGAGCTGAGTTTCGAGAAGACGGTGGAACGATTTCCCTACTCCGTAGTGAAATTTGATATCGCATATCCGTATGGGGAAAAGCATGAGGCCTTTACCGCCTTCTCCAAATCTGCCCACAAGGCAACTAAAGATCTGCTAATAGCCACCGTGGGTGTCAAGGACTATGGAGAACTGGAGAACAAGGTGAGTTTTATTTTATGGATTACCTAGGATTTAGATATGTGGTAATGATTGGAAAAAATGTGGTTTCTGCTCTGTTCATTTGGAATCCTGGATTGCA

GCCCTTCTAAAGACTCTGTGCTATTTAAATTATTTAAAAGCACTTTAAATTTGATTTATTTATTATTTCAACTTTTTAATCCCAATTTGCAGGCCCTGGGCGACCGTTACAAAGTCGACGACAAGAACTTCCCGAGTATCTTCCTGTTCAAGGGCAACGCTGATGAGTACGTCCAGCTTCCTAGCCACGTCGACGTAACGCTGGACAATCTGAAGGCTTTTGTCAGTGCCAACACACCTCTGTACATCGGTCGTGATGGCTGCATCAAAGAGTTCAACGAAGTGCTCAAGAACTATGCCAATATCCCCGATGCAGAGCAACTGAAGCTTATCGAAAAGCTGCAGGCCAAGCAGGAGCAGCTGACCGATCCCGAGCAGCAGCAGAACGCCAGGGCCTATCTGATCTACATGCGGAAGATCCACGAGGTGGGCTACGATTTCCTGGAGGAGGAGACCAAGCGACTCCTGCGCCTCAAGGCCGGCAAGGTCACCGAGGCCAAGAAGGAGGAGCTGCTGAGGAAACTGAACATCCTGGAAGTCTTCAGGGTTCACAAGGTCACCAAGACAGCGCCGGAAAAGGAGGAACTGTGAACTTGTTAGATCTTTAAACAAAATTATGAATACTTTATGATATTTATAAATGTCGGATCTCGACAGAAACATGTGAATGTACAATTTATACGAATTATAAGTCTATCATCCAAATAAAGACAGTTTTTTATGTTGAAATGCCTTGTAGTAATTAAATCAAACTATTTTATTTCCACTCCTCATTGCCAGTTAACAAGTCAGTTAAACACAAATAAGCCTTCAATTTATAAGTCCTCTGGTACATGCATATTTACATTTACATAGAACTTCGTTTTACCATTAATTAGAAAGTCTAGGCGTGATAAATAATCTCCAGAGGGAAATGGAAGGGGTAGTGAAATGCGATGAAAATCGTTAAGTACCACCGTACCCTGCAAA

 

---

Search Again