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CRIMIC CR00963

Gene CG17002

CG17002

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6987556..6987534	42E1	FBti0213019	91250

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CCGGTCGTCCACGAACGCTTATAAAA	TAGGGGTTAGAGGCTAGTACAATCGC

Images

Construct Design Sequences

sgRNA sequence	TGGATTTCTCATGAACGGGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGACCGTCCAACTCAAGA	TGAAATGGATTTCTCATGAACGG	TCACTGCGCACACTAACCTC	GAGAATGAGACCCACCTGGC

 

Amplicon 5'

Amplicon 3'

GCTGACCGTCCAACTCAAGAAGGTGCTCAACGAGGACGAAACGCGCAAGAAGCTGGCCAAGGAGAACGAACTGTTCGCCATCCAGCAGGCGGCGGCCAGTAGCCCGGTCTTCCTGCCGCCCCTGCGCCTCCAACACCAGCACCATACGCTGATGCAGAAGGTCTCGACTGCGCAATTGTGGGATTCAGGTTCTAATGGTCCTCTTTTTTCCTCGCAGCTCCCGTCCGGCGGACAGCCAGGAAAACGTGGCAGGAGTCCATCGCCGCCGAGTCAGCAGCAGGCTTACTACAAGAGCGCCGCCAGCTACGCCCAGCAGAGTAAGCCAGGAAGAGGTCGACAATGTGGTCCCTGGGCCTCCGATACAGTGAACGCTGCCTCAGGCAGGCAGTCCAAAGTCTCATGCACGCAATTTCGGTTGGATTTTGTTAACTCGAATGCGGGATCTGCTCATAATTTGATTAGTCATTGAATTGGTTAGCACATATGTATGGTTCTTGATTTGGACGAGAGATATACGTGATTTTTGTGATGTCATCATCTCTACATTCTGATTGCCAAGCCCTTTTTTAAGCTCTATGAAATATTAGCACTAATATCAATTGAACTTCTAATTAGGAATGATTGCAAAAACATGGTTAAGTGTTAAGTAAATAATCAATTCGATATAAAGAAAGTTTCGTGCTGTTTAGGCTTTGTATTGGATGGTTTAGTTTAATGTGTTTTAATGTGCACAAAAATTACCCTATTATTTTGAAGGATATATAGTGTAGAAACGATATATATAGGGTTTCTAGCAGATAGCTCGAGTTTTAAAATTCAACTGCAAATTGGCATTGGCTAAAGGTGCGATACAATCACCGATTGATCTGTTCTGCGGAGCTTTCGCTGTTCTTCTTGATTAACTGCTCGCTAACACTCCGTCCCGTTCATGAGAAATCCATTTCA

TCACTGCGCACACTAACCTCTAACAACACAGGCACACAACTTATGTTCATTTCGTTATTTGTTTACGATCGATTCGCAGAACACGATGACTACCGTCGTGCCGCTGACTATGCTAGATTATCATGGAACAGTAAGCACCTCACTTGCAATACCAAGCCCGAGATCCTCCGTCGGAACGATCGCCAGATTCGTCAATTAAACCTCACTTTTCCTCTCTTCCAGAAACAGCGGCACAGTATCCGGGCACGGGAACGGTCTTCTACCAGACGGTCGCTCCTCCGCCGACGACACAGCACCAGGCGGACGCCCGCTTGCAGTCCATCTACAACTACAACCTTCCGTTGCGCCAGGCCTACCACGTGGACCTGCCCAGCGCCACGGTCAGCAAGCCGCCCGACTCGCAGTCGCCCAAGGCACCGTCGCAGTCGCAGCCCATGCAAGTGCTTCACATCAACCTCGACCAGCCGACCATTTCGCAGGCAGACCTGGTGGCTCAAGCCGGCGGCAGCCTCTCGGTAAAGGCCTCCCAGCCACACGTGACTATGGAGAAGCTACCGGACCGCTACCACATCGAGGTGAAGCACGACGGCCAGCCGCCGAGCCACGTCCCGCCTCCGCCACACCTGCTGCCGGAGGGCGTCATCTTCAAGCCGCTGCTCAACGAACTCTCATTGCACAGCAACGTGCTGCAGATAAGCAGCAGCCAGGTGGGTCTCATTCTC

 

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