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CRIMIC CR00966

Gene CG3558

CG3558

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:2997269..2997272	23C4	FBti0206860	83220

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGGCATATCTCTTCGAGGCTCATTG	ACTTAAGATCCACCGTCCCGATTCAC

Images

Construct Design Sequences

sgRNA sequence	ATTAACCAGGCTCTAATGCAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGCGCGACAGTTCTTTAGT	CAGGGCTCAAGTACGTGTGT	TTAGAGCCTGGTTAATTGCCA	TGCGAGGATTAGCAACAGCA

 

Amplicon 5'

Amplicon 3'

TGGCGCGACAGTTCTTTAGTGAGTTCGGTGGCGGTCCTATCGCCGCCAACGAGACGGGTGAAAAGCAGCTGGACAGCTTGCAGTCAATTGGCGAGTCAAGCGGCTACGAATCCTTCAAGTGGCGGCCAGCGGACGAGGAAAGCGAAGCCACGGATACAACTGTGGCCACGACAGCCAGCGAGGCGGATATGGATCACAATAGCAGCAGCCTTAGCAGCGTTCTTGGTGCATCCGGCAAACGCGAGTCATGGCGGACTTCGAACAGCAATCGCAATGAATTAATATTGACGGACCTTGACTTCTCGGAAGATTTGTTTGCGCAGGGCACCGTAAGCTTAGGTGAGAATTAAATTGTTCTTCTTAATTCCTTATAAATAATTGCTATGTATCAATGTTTATTTCCAGGTCCCTTTCTCAATGCCATTTGGGGCAAACTGCAAACCTTCACGAGCAATTCGCTGTACGTCAATCTCCACTTGACCGGGCTGATCACTCGCTTGGCCTGGTATCCCCTGCCGTTGATTCACTCGCTGCTGCTGCGCTCTGACATAGCCATCACATCGGATACGCCCTCTTTTCACCAGGTGCTGCGCATTCTGAAGCAGCAAATAGATGCCGAGCTGCCAGTGACGGAGGATTCGCTGGAGATCATTGATGTGGCGCGTTCGTCGCTGATTGATCGAGAGTTCCGCTTGGCAAACGCGCGCAAGGGCAACGAGGGCTCTCCAATGCATCATAGCCAACAGCAACAGATGGTAACGAACTCCGGCCAGCAGCAGGGTCAACTGCGATCAGCCTACGCGACCCTCTCGGCCGCCACGCCCGTGCAGGCCACGCCCACCAGTGCCTACGATCCGTTCAAGCGCAGTGATAACAAGCGTCGCAGCATCAGCAAATCCATTACCAGCATGTTTAGCAGAAAGTCCGCCTCCACGTCCACAGCGCCACCCAATGGATCCTCCGGTGAGTAACAGAATCGGTTGCCTGCTTTTGTCACACTCTTGCACAACTTCTATATTTTTCAAAAAAGAAAAACCAAGCAAACAAACTAGATATTACCAACAGTTTACAAATCTGCAGCGATGGATGTTGGAAGAAAGTTGGTTTTGGGTCACATTACACACACACTTCACTCACAAAAACACACATTCACACACACGTACTTGAGCCCTG

TTAGAGCCTGGTTAATTGCCAGCTAATGCAAAGTAAACCAAAGAAATCATTTTAACATTTAAATGAACTATTAATGTTATAAGAGTAAACAGGTCTCATTCAGGGCTAAATTTGGTTGACACACATCTCTGTAAAATGCCGCCGGCATCGTTTATAATAAATTAATTTCTTTTATTTCTTTATTTTTATCGAATCGATCAAAAAATCAAACAAATCTTAGCTTCATCTGGCTTATCACAAATTTACGCATTCTTCACCGGTAAATATTTCACGCTTGATAAAATATTTAAAGTTTTAATCAAACCTAACCCAATATGGCATAGTCTTGTTAATATTTGTGCGTAGTATCTTTATTTTCGTAGAGCTTTGTACATTATGCTTTCCACTAAAAAAATAGTTAATAGTAGAATATAGAAAACATTTGTGGAATCCCATATATACATGTGGTTATTGTTGTTTTTTAATTAACCCAAAATGTCAATATCATGTGCTGTTGCTAATCCTCGCA

 

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