CR00969

CRIMIC CR00969

Gene Atg4a

CG4428

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1081438..1081469	21E4	FBti0206861	83221

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCACTTCCATATGCAGGAGATCTT	ATGGCAAAAGAGTCCGAATCCGAGGT

Images

Construct Design Sequences

sgRNA sequence	CTTATTTAATGCTGTCATATGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATGGTCGAGGATGCCGATC	TCTCATCTTACCCTGTATGGCG	TCTAGCATATTGGTCAGCATGA	CGATCATGCCACAACTGCTG

Amplicon 5'	Amplicon 3'
AATGGTCGAGGATGCCGATCCAGAAAACGACTTCTTCGATCTGAACAACAAATTCTGCGCACTCAAGGTAGCGTAGCTAGTTTGAAGTCCCGCACGTGCAATTTTGAAGTTTTTACATTGTTACAAATCTATACTTTGCGTAAAATAAGAAAAAAATTAAATATTTTTTAAAAAACTTCATTTTTGAATGCATTTCATTAACAGTTTGGAAAAATACTGTTATTTCCTATGTTATGGTCATGTTAAGTGCAAGGTTCGGTATTTTGGCAAAGTAAAATACCTTATCAGTTGCGGTCATACTAACAGCTACGTCACTGCGTTGTAATCAACAAAAAAGAAAAATAAATCAAACTAAACCAGCCGCAGACTGATCAAATGCTGGTTATCGCATAGTTTGTAATCAGCGCCCGTCACTGCCAACAACAAATGAAACCCCTGTGACCTCGGGCCAGAAGCAGGAGCATTGCCGTTGCTGCCAGCGAAAACACAGGCCAAAACAAAGGCCCACCCGAAAAACAGAACGTCATTCGAAGGCAATTATCGTATAGACGAGCAGCGGAGGCCCTGGTGTCCACCCCCTTCTCCTTCTGCCAGCGCACCGGGCAGTAGATGCTGGTTGGACTCAGCGACCAGCTAGCGCGGATCATGGAGAGCGTCTTCGAGGCGTACTTGGGCCCAGACAGCGTCCTCGCTAGTGCAGTTGGCCAGGCGGTGGGCAGCGGAGAGCCGGAGGACATTCCCCGAAGAAACACCGACGTCTGGGTCCTGGGAAAGAAGTACAACGCCATACAGGGTAAGATGAGA	TCTAGCATATTGGTCAGCATGAGTCATAAGGGGTTTCCCAAACCTTTTTTCGATTATCATCGATGGATACCAAACTGAAATCCATAATCAACACCCTATCCCTTTTCAGAGCTAGAGCTCATCCGACGGGACATTCAGTCGCGCCTGTGGTGCACATATCGCCATGGATTTTCGCCGTTGGGAGAAGTCCAGCTCACCACGGACAAGGGCTGGGGCTGCATGCTGCGCTGTGGTCAGATGGTTCTCGCCCAGGCCCTGATCGACTTACATTTGGGACGCGACTGGTTCTGGACGCCGGACTGCCGTGATGCCACCTACCTCAAGATCGTGAACCGTTTTGAAGACGTAAGGAATAGCTTCTACTCCATACACCAGATTGCCCAGATGGGAGAGTCCCAGAACAAAGCGGTCGGCGAGTGGCTGGGACCGAATACAGTAGCCCAGATTCTCAAGTAAGACCATTGGCCTAACATGGTGGATAAGTGGTATAATCAATTGCTGATTTCAGAAAACTAGTGCGCTTCGATGACTGGAGCTCCCTGGCGATTCACGTGGCCATGGATAGCACAGTGGTATTGGATGACGTATGTAAGTGTGGAAAATGGCCTAAATAGCCCGACTATTTGCCTAACAACATTGTCCACTTTGTAGATGCGTCGTGCCGAGAAGGAGGCTCGTGGAAGCCACTGCTGCTCATAATCCCCCTGCGCCTGGGCATCACAGACATTAACCCGCTTTACGTGCCTGCCTTAAAGCGCTGCCTTGAACTAGACAGCAGTTGTGGCATGATCG

Amplicon 5'

Amplicon 3'

AATGGTCGAGGATGCCGATCCAGAAAACGACTTCTTCGATCTGAACAACAAATTCTGCGCACTCAAGGTAGCGTAGCTAGTTTGAAGTCCCGCACGTGCAATTTTGAAGTTTTTACATTGTTACAAATCTATACTTTGCGTAAAATAAGAAAAAAATTAAATATTTTTTAAAAAACTTCATTTTTGAATGCATTTCATTAACAGTTTGGAAAAATACTGTTATTTCCTATGTTATGGTCATGTTAAGTGCAAGGTTCGGTATTTTGGCAAAGTAAAATACCTTATCAGTTGCGGTCATACTAACAGCTACGTCACTGCGTTGTAATCAACAAAAAAGAAAAATAAATCAAACTAAACCAGCCGCAGACTGATCAAATGCTGGTTATCGCATAGTTTGTAATCAGCGCCCGTCACTGCCAACAACAAATGAAACCCCTGTGACCTCGGGCCAGAAGCAGGAGCATTGCCGTTGCTGCCAGCGAAAACACAGGCCAAAACAAAGGCCCACCCGAAAAACAGAACGTCATTCGAAGGCAATTATCGTATAGACGAGCAGCGGAGGCCCTGGTGTCCACCCCCTTCTCCTTCTGCCAGCGCACCGGGCAGTAGATGCTGGTTGGACTCAGCGACCAGCTAGCGCGGATCATGGAGAGCGTCTTCGAGGCGTACTTGGGCCCAGACAGCGTCCTCGCTAGTGCAGTTGGCCAGGCGGTGGGCAGCGGAGAGCCGGAGGACATTCCCCGAAGAAACACCGACGTCTGGGTCCTGGGAAAGAAGTACAACGCCATACAGGGTAAGATGAGA

TCTAGCATATTGGTCAGCATGAGTCATAAGGGGTTTCCCAAACCTTTTTTCGATTATCATCGATGGATACCAAACTGAAATCCATAATCAACACCCTATCCCTTTTCAGAGCTAGAGCTCATCCGACGGGACATTCAGTCGCGCCTGTGGTGCACATATCGCCATGGATTTTCGCCGTTGGGAGAAGTCCAGCTCACCACGGACAAGGGCTGGGGCTGCATGCTGCGCTGTGGTCAGATGGTTCTCGCCCAGGCCCTGATCGACTTACATTTGGGACGCGACTGGTTCTGGACGCCGGACTGCCGTGATGCCACCTACCTCAAGATCGTGAACCGTTTTGAAGACGTAAGGAATAGCTTCTACTCCATACACCAGATTGCCCAGATGGGAGAGTCCCAGAACAAAGCGGTCGGCGAGTGGCTGGGACCGAATACAGTAGCCCAGATTCTCAAGTAAGACCATTGGCCTAACATGGTGGATAAGTGGTATAATCAATTGCTGATTTCAGAAAACTAGTGCGCTTCGATGACTGGAGCTCCCTGGCGATTCACGTGGCCATGGATAGCACAGTGGTATTGGATGACGTATGTAAGTGTGGAAAATGGCCTAAATAGCCCGACTATTTGCCTAACAACATTGTCCACTTTGTAGATGCGTCGTGCCGAGAAGGAGGCTCGTGGAAGCCACTGCTGCTCATAATCCCCCTGCGCCTGGGCATCACAGACATTAACCCGCTTTACGTGCCTGCCTTAAAGCGCTGCCTTGAACTAGACAGCAGTTGTGGCATGATCG

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