CR00970

CRIMIC CR00970

Gene CG15356

CG15356

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1980510..1980529	22B8	FBti0206862	83222

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTTGTTGCCGAAACTCATGTCGGC	GGCACGTAAAGTTCACCCTCTATCGA

Images

Construct Design Sequences

sgRNA sequence	CCGATCCTCAAGAGCTCCCGAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGAGGGCTTCTCCAAAGT	GAGCTCTTGAGGATCGGCC	TTCGTTGACCACTCAGCTCT	TATGCGTACCGTGGCTTACC

Amplicon 5'	Amplicon 3'
CACGAGGGCTTCTCCAAAGTGGACCGCCGTTTCGGCCGGAGCAGCCAATTTGGCGTTTTCCTCGCAGGCTTTGGCCGCCAGGTCATTGGCAACGATGCTCAGCGCAGTTTGTGGACTAATCCTGGGAAGAAGAGGATAGAGGCGTCGTCCCTCGCGTGACCATTCCTGCCAGGTGTTGGGCCCACAAACGCTGCAAGGGCAGTACAGATACACAGGGCATTAGTAGTTAGTTTGCACTGGAGTATCTCACTGGGAATCTCACTTTTCCGCTATGGTGCAGAGCTGCTCGTCGTTGGCCGCCCTCCGCACTTCCGCCCTGTGGCGTTCCTGGTTAATGTGGAACTGCTGGCGCAGCTCCTCCAGCAGTTTCGCCTTCGTTTCACTGAGGGCTCCTTGGGCGCGAAAAACGCTCACCACATGTGAATAGGACTCGAGTTCTGGTGGGCACAAAAACAGAGACAACAGGAAGAAAGTGCTTTAGTCGCCAGCGCTCCACTCGGCGGCCGATCCTCAAGAGCTC	TTCGTTGACCACTCAGCTCTCACCCAGTCGACGCAGCATGCCTCGGCACTCGTCCCGCGACATTTCCAGCGTTTGCGGCCACATCCCGACTTTCCCGTGATTTACGTCCTTGGCCGTCGGCGATCCCGTGCTTTTCCGGTTGCTTGAGCTTTGGCCAACGCAGGATTAGGTCGCTAATTCAAAAAATACGGATAAATGGTTTGCTATTCCTAATAAAATAAAGCAATAAGAATATCGAACTATCGATGGGCGATAGGCCGCTGCGGTCACACTGCGGCCAGGAGGAAATAAAGGTATCAAACTATCGCAGGTGGCAAGGCAGCGATAGATCGGATAGAAAATAACAAATTTAATTAAAAATGAAACTAAACATATAAATAATTACAGTGCTAACTTATTACTTGTAATGCCAAGCAATGGCATTAGTGATTTCATTAACGCTGCTATATGGAAAGGTGATTTATTTCGCACACCTACAGTTTTTATATATTTTTAATTTCCATATATTTTCAATGATTAGTTCTGGATACATTTTCATATTTTATAAAATGTAAATACACGATTTAAAATTCCCGCCTAGCACTGGAATATTTCCAATGGAAGTGCACATAGGACCGCTCGATGGTTAATCGATAAATACCCGCTACAATGTTTACTTCGCTTATCCAAAATATTTTACCAATTCGAAAGTGTGAAACGATCTTAGTCACAGACGTTCATGAAAAGAAAAGCTAAGACCGTGCCGATCCAGTCAAACAGCCGAATGAAAAACAAGGCTAAAATGTACATAGCCGGGCTGGCCGGTTTGGGCTTAATAGTTTTGGTATCGATCTACAAATGTGCCAGTGGCGAGGTTCGCCAGGCGGAAAGCGAAAAGTGCTTCTTCTGCGACTTCGCCCACCGCCGCCAAGGACCGCCGCCCATCCTGGAGGTGGAGACGGACGAGTACGTGATCTTCAAGGACAAGTATCCGGCAGCCAGACTCCACTATCTGGCGATTCCCAAGGAGCACTTCGACAGCCTGAAAGCGCTGAACAAATCGCACGTTGGATTGGGTAAGCCACGGTACGCATA

Amplicon 5'

Amplicon 3'

CACGAGGGCTTCTCCAAAGTGGACCGCCGTTTCGGCCGGAGCAGCCAATTTGGCGTTTTCCTCGCAGGCTTTGGCCGCCAGGTCATTGGCAACGATGCTCAGCGCAGTTTGTGGACTAATCCTGGGAAGAAGAGGATAGAGGCGTCGTCCCTCGCGTGACCATTCCTGCCAGGTGTTGGGCCCACAAACGCTGCAAGGGCAGTACAGATACACAGGGCATTAGTAGTTAGTTTGCACTGGAGTATCTCACTGGGAATCTCACTTTTCCGCTATGGTGCAGAGCTGCTCGTCGTTGGCCGCCCTCCGCACTTCCGCCCTGTGGCGTTCCTGGTTAATGTGGAACTGCTGGCGCAGCTCCTCCAGCAGTTTCGCCTTCGTTTCACTGAGGGCTCCTTGGGCGCGAAAAACGCTCACCACATGTGAATAGGACTCGAGTTCTGGTGGGCACAAAAACAGAGACAACAGGAAGAAAGTGCTTTAGTCGCCAGCGCTCCACTCGGCGGCCGATCCTCAAGAGCTC

TTCGTTGACCACTCAGCTCTCACCCAGTCGACGCAGCATGCCTCGGCACTCGTCCCGCGACATTTCCAGCGTTTGCGGCCACATCCCGACTTTCCCGTGATTTACGTCCTTGGCCGTCGGCGATCCCGTGCTTTTCCGGTTGCTTGAGCTTTGGCCAACGCAGGATTAGGTCGCTAATTCAAAAAATACGGATAAATGGTTTGCTATTCCTAATAAAATAAAGCAATAAGAATATCGAACTATCGATGGGCGATAGGCCGCTGCGGTCACACTGCGGCCAGGAGGAAATAAAGGTATCAAACTATCGCAGGTGGCAAGGCAGCGATAGATCGGATAGAAAATAACAAATTTAATTAAAAATGAAACTAAACATATAAATAATTACAGTGCTAACTTATTACTTGTAATGCCAAGCAATGGCATTAGTGATTTCATTAACGCTGCTATATGGAAAGGTGATTTATTTCGCACACCTACAGTTTTTATATATTTTTAATTTCCATATATTTTCAATGATTAGTTCTGGATACATTTTCATATTTTATAAAATGTAAATACACGATTTAAAATTCCCGCCTAGCACTGGAATATTTCCAATGGAAGTGCACATAGGACCGCTCGATGGTTAATCGATAAATACCCGCTACAATGTTTACTTCGCTTATCCAAAATATTTTACCAATTCGAAAGTGTGAAACGATCTTAGTCACAGACGTTCATGAAAAGAAAAGCTAAGACCGTGCCGATCCAGTCAAACAGCCGAATGAAAAACAAGGCTAAAATGTACATAGCCGGGCTGGCCGGTTTGGGCTTAATAGTTTTGGTATCGATCTACAAATGTGCCAGTGGCGAGGTTCGCCAGGCGGAAAGCGAAAAGTGCTTCTTCTGCGACTTCGCCCACCGCCGCCAAGGACCGCCGCCCATCCTGGAGGTGGAGACGGACGAGTACGTGATCTTCAAGGACAAGTATCCGGCAGCCAGACTCCACTATCTGGCGATTCCCAAGGAGCACTTCGACAGCCTGAAAGCGCTGAACAAATCGCACGTTGGATTGGGTAAGCCACGGTACGCATA

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