• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00971

Gene CG15439

CG15439

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:4450749..4450749	24F3	FBti0206928	83223

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATATGATGCAACGGGCGAGGGAAA	TATTTCTTGGGCAGTGCATCCTCGTC

Images

Construct Design Sequences

sgRNA sequence	TCGTTGTTGAGAATTCGATTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATTGAGACTCCGGCGCATC	TGAGTTCGTTGTTGAGAATTCGA	CGAATTCTCAACAACGAACTCA	ATGTCAGTGGATGTGCAGCA

 

Amplicon 5'

Amplicon 3'

TATTGAGACTCCGGCGCATCCTCGCCGGGTAAACGCAATGCCTTGAATATAATCTTAATGGTGCTGCCCGAGGTGTTGCCCGCGTTATCCAAATGGGTGGAAGACTCGTCAAGGTGGGCGCGCTTTTTTCGCTTCCGTTTATGCTCCTTGCCGATCGATTTCTCTGTATCCGACGACAGGTTGTGTTTGTTTTTGTGCTTGTCCTTGCGCTTACCCTTGCGCGATAATTTTCCACTGGAGTCGTCAATCGAGTCCTCACAGCCAGATAGGTTAATCACGATAGTATTGGGCGTGGGCAGTGTTGGAGCTGCCGCAGGAGTAGCCGCCGAAGCGGCAACAGGAGCAGCATCAGTTGTGGGAGTGACATTTTCGGCAGGAACTGGCGGAAGAAGATCCGTGACATTAGTTTTATTATTTATTATTAAACATACTTGAATTTGCGCAAAAAATTTTTTCTTTATTATTTGTTAACAAAAAAATAATTTCTTGTATTAAATACCAATTGGGTATAATTCAATAAACGTAAACAAAGATCAATGTTTGAGATAGTTTTGACTTTCTTTGTACAGGCCAAAAAAAAAACAAGTGTAGTGATAATCAGGATTAAACAGAAAACCAATTTCTTCATAAATCATAAATTTCTCTAACCATCAAGCGAAAAATTCCGTTAATTCACGCATACATATTTACACATTTGCATTTAAAAAAAAATAAACCAAAAACTCCAAATCAGCAATAATAATCTAAACAGGTATTGTGCCAAATCGAATTCTCAACAACGAACTCA

CGAATTCTCAACAACGAACTCACCATTAATTGGTGTTTTACATGGGGATTGGAGTTTAGGCCGTTTCGGCGACTTAGTCAGATCTTCTATATATTCCGGAGCAGGAGCTGGCAGGGATTTGTTGAGCGCTCGCTTGGTGTGTGACTCCTTGGGTACATTGACAGCGGCCACTGGAATATCGTCCAGCGAATATCGATCCACATAGACCAGATGGCCATCCTTATTGAAGCGAGTGCGTGACTTTCTCGGACCCGATGAGATCTCCGTGACGGCATCCGAACCCTCAGACTTGTCGCAGCATTCGGAGCACTGCCAGCCATACTGCTTGGATTTCTTTGGGGGTCGAGTGAGCGGCGGATTCAGACAGCCCAGGTGGTAGTGCAAATTGCAAGTGTCACATTTGACCAACAGATGCTGATCCTTGCTCCTCTTGCAGATGCCACAGGACACAGAAGGGGCCGCCTCCACGGATGCCGAGGAAGTGCTGTGTTTGCCCTGTTTGGCATGAGTACTGAGCATGCGTGACGAGTCCATTTTACTGCCCGGTGGCCCCAGATGACCCAACGGAAAGCCCACGCCCATTTTCAGTGCAGCTGCCGTGGGCACCGAGATGGGACGCTGGGGTGGAGTCGGCGTACTGGTCTTTGGCGGCGCCTCTGGCAGTGGATGCGCAATGAGATCCACGCTGGGCAGCGTCATGTTGGGTGCAATTTGGCCCAAAGCCGTGTGCAGAGACTTAATGCTCGTTAGCAGGGAATCCTTAGTGGCCTTCACTGCCTTGCGCTCCTCCAACGCGGTGTCGTATTGAGCTCTCAGCACGTCTTGATCCTTGGACAAGATAGCGTTGTGCGAAATAAGCTCCCGCTGCTGCTGACGGAAGTCATCCATGCGGACTATACGGTCCATGTAGTAAGCAGTGAACTCAACGCTGAATGCCGGCGCAATGTGCCACTTCTTCCGAATGTCCGTCAAGGCGTTAATGTGCGCCTCGCGCTGCTCCAGATGCTGCACATCCACTGACAT

 

---

Search Again