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CRIMIC CR00972

Gene Sr-CI

CG4099

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:4122631..4122652	24D6	FBti0206863	83224

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCCATGGAGACATTGGTATCGACGA	GCATAACGGCTGATCATCCATGCTGT

Images

Construct Design Sequences

sgRNA sequence	CGCAGGCAATGAGATAAAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGTGCCAGTAAGAGCCACA	TGCCTGCGACCTTTAATCTAA	AATTCCGAAAGCATGGCAAC	GACGTTGGCCAGAACTACCA

 

Amplicon 5'

Amplicon 3'

CTGTGCCAGTAAGAGCCACAAAATCGATATAATCTAATGGTTTTACTGAGTTTCTTGCATTTTTTCAACATAAGGTAGGGGATGTGCGAGGCCCGAGGATCCGGAGAACGGACACGTCGAAAATCTTTCCCTAAGGGCGGATGTCGTGTGCCACGATGGCTATGTCTTGGTCGGTGGTCGCACTGCCTACTGCGATGGAGAAAGATGGAGCACCCAGCTGGGATCGTGTCGAAGGAGCAACCACACAAGAGATCATTCTTGCGATTTCGAGAGCGAGGATCAGTGCGGTTGGGAGGCGGAGACAACCTTCCGACGACCCTGGAAGCGAGTCAGCACGGTATCCGATATTCACTCCCTAAGAACGGGACCCCGCCACGATCACACGTTTAAAAACGAATCCGGTGGTCATTACATGCGCATGGAAACCCAAATGGGGGCTTATGGAAGCTACCATCTGCTATCGCCGATCTATCCCAGATCCCTCACCCTGAAGACCGCCTGCTGCTTTCGATTCCACTACTTCATGTTTGGCGCTGGTGTGGATAATCTGGTGGTGTCCGTTAAACCCGTTTCGATGCCAATGGCAACCATGTGGAATAGGTTCAGAGCCAAGTGAGTTTTGCCGGAGCGGAAGAGGTTTTAGATTAAAGGTCGCAGGCA

AATTCCGAAAGCATGGCAACAATTATACTGATTGCTTCTGTTTCTGTTTTATCTTAGTTGCAGCAAATTTGAGATATCTGGTCAGCAGGGAACCCAGTGGCTAGAGCACACGATCAGCATTGACGAGATGCAAGAGGACTTCCAGGTGATATTCACGGCAACGGATGCAAGATCCCAATTCGGAGATATTGCCATCGATGATGTAAAGCTAATGACAGGCAGTGAGTGTGGCACAAACGGATTTAGCACCACCACAGAACCAACGGCTCCGACAGGCAGCAACGAGCAACCACTGGTCTACGATATGATGAGTTGTTCAGGTCGATGCGGAACATCAATCTCGGCCTCCAATATAACCAACAATGGTATAGTCATGGGATGTGGGTGTAATGACGAGTGCCTTTCGGATGAGACTTGTTGCCTAAACTATTTGGAGGAGTGCACAAAGGAGCTGCTCACCACGACCGAGGATGATATTAGTTCCCTGCCCCCAACGGTCACATCAACAAGCACAAGCACTACGAGGAAGTCAACGACAACAACAACCACAAGCACAACTACTACAAGTACAACAACAACTAAAAGGCCAACCACAACCACAACAACAACAAAGGCCACAACTACAAAGCGAACAACAACCACTAAAAAACCGACAACAACTTCAACAACGCCGAAGCCAACAACAACGACTTCAACCACACCAAAGTCTACAACTTCTACAACGTCTACAACTTCAACAACACCAACGACAACAACTACAATAAATGTGTTTACAACAAAGAAAACAACAATAATGATCCCTACTTCCAGTACCGAAAAGACTACAGGTAAGACTTAAGCCTTTTTTTTATGATTGCGTTAAAAGAAAATTAATATGATAACCTAGGCATCAACACCACAATGAAAACACGGAAGCGCATCACTTGGAACGTTGATCCTCAGGACATCGAGGGTCACATGGACACGAGCGGAAGTACCCCCAATCCAGCTTTAGTAGTACTTTACCTGCTCCTCGCCATTGTTCTGGTGGTAGTTCTGGCCAACGTC

 

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