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CRIMIC CR00973

Gene l(2)k05819

CG3054

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:4396742..4396747	24E5	FBti0206864	83225

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCAAATCGTTCGAAGAGGACCTGG	ATCCCCACTATTGAGGGCGTTAAGTG

Images

Construct Design Sequences

sgRNA sequence	CGTGAGCATGCAGGCAAGCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTCTCCACTCACCCACGAG	GTGAGCATGCAGGCAAGC	CACCGACGCATCCCATCC	CTGGGCCGTCACCTACAATT

 

Amplicon 5'

Amplicon 3'

GTTCTCCACTCACCCACGAGCACTACGCACAGTTCTGCGGTCATGTAACCGCGGTGGCTATGCTCGACAAAGATCTGGTACCTGTGACTAATCGGTATGCGTATGCACTCATTGAGTCCAGTAAAAGCTTTTTGCATGGGTACGTAGTTCATTGCCATACTCATCGTTAGTTTCTGTCTAACCAGTTATATATATGGTCGTTTGCATCGACCGCAAATATACTACTTATATAGTCTAATGATTTTCTCCAAAATAATGGTATTGAATACCATTGCATCCAAAGCCAGCCCGATCAAATTAGTCTTTTCTTTGGGTATTTTTGATTTTTGAATCCAATTGGACGATTTCCCTACAAAACAAGCACAAGAGATTGGTAAAGATTTAAAAATTACAAGCGTAAGAAAAAGTATTTTTGATTTCCAGTAACTTGCTATTCCTTAATCGTTTTGCTCTATAATTTCCCATATCATTCAAGCCTAAAAAGTAATTGCAATGTGGACATGTAAACCTTGTATTTATGTATTATTCTACCTCTATTAATTTGTTCGTACTCGTAAATCGATCTCCTTCGCCTCCCCGCCCCATCCCCATTGGCATGCTATTTTAAGTAACAGACATTCAAGAACGCTTAAATATAGTCGCCCCATTCCCGCCACCGAAACCGCCCCATCCGTCGACCTCCGTCCGCCACGCTTGCCTGCATGCTCAC

CACCGACGCATCCCATCCAGCGGTAGTTGGAACCAGTTCTAATCGGTTGTTCTCTATAAACAGACGCTGCCTTTGCGAACTGGCCAAACAGATTGGATTCACCGACCATGCCACTGATCGGTTCGCGCTGGAGGGTCAATTGGCTAGCTATCGCCACTTGGTAAGACTCGGTGACGTTGTAAAATAGTATTCTAGTTATTATTTAAAATATCTCCATTGCAGCAACCTGATGTTGTGCGAAGGGACATTCGTTTCGCCCGGCAGCTCCAGCTGTCCTCCAAGGTGAAGGTCCCATTCCCGCACTCTTTGTCTGTGGTCATGCGCGAGAATCCAGGCGGCTATTTATCACTTTTCACCCAGGGAACAGCAGACATTATACTGGACTGCTGCGATGACTTTTGGGACGGCATTGATCTGCGACCATTGTCCGCACAGGATCGCAAGCGTGCACTGGACTTTTATCAGCGCAGCGCGCTGACGTCCTATTGTACAGCGTTTGCCTATCGACCTCTAAGGCACGGCATCCATGGAGCCCTTAGCGGTCCTCAACGGAGTGGAGGTGACTGTTTGTATCTGGAGCTACCGCCGGAGTCAAAGCTGCGCATGCAGCACGTGGACAAGACCCATTGCGACTTCCAGGGCGGACATCACGTCAAGCTGAGCCACAGCATTAGCACGGACTCATTGCTATTTTCGGAGAACAAGGACGAGGAGTGTAACGATGTGGAGGGCTGCTTCGAGATGCAGTGCCATCAGGTATTCATCGGCATGGTGACAATGCAGTACCAGGCGCAAAACGATATCGTACGACTCGTTGAGCATCTCGAACAGGCCTGCATACGCTTTGTTCACTTTAGCAAAGAAAACGAACTGCGCTCGCGTGTCTTCTCTGAGAAAATGGGTCTAGAGTCCGGATGGAACTGCCACATCTCGCTACTGAGCGAACCTGAGCCGGATAGTGAAAAGGAGCGGATAAGTGCCAAGCCAAAGGAGTTCCCGGAAAATATTGGTAAACCCCAAGCCACCACCTCAACCACTGCCACCACCACTACAACAGACCATGAGAACAACCATCATCCCAGTGCCAAAGTCATGTACATATCGCCCCCCGCACCCGCACCTGCCACTGCATCTGTATCCGAATCCATATCTGCAGCTAAAACCACTGAAATTGTAGGTGACGGCCCAG

 

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