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CRIMIC CR00975

Gene CG9967

CG9967

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:2309816..2309816	22E1	FBti0206929	83227

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATACACGGTACCCATGACCAGCCATA	GAGAATGTCGCACCCCCAATGCAATA

Images

Construct Design Sequences

sgRNA sequence	CCAGAACCATCTCTAAAATTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGTCGTCGTCCTTGGAACA	TGGCAGCCAGAACCATCTCT	TAGAGATGGTTCTGGCTGCC	GCTTTTGGTCGCAACTTCGT

 

Amplicon 5'

Amplicon 3'

CTGTCGTCGTCCTTGGAACAATGCACTGCTCACAGAAGGCGGTCTTGCTGCGGGCGGCCTCGCTCAGGTGGGCGTGGCCGAGGGCCTGCAGGAAGGCCAGGACGAAGGCGTAGCAGTTGTGGCTCTCCTCCGCATAACTGGCGATGGACCAGCGCACCGATTGGGCGCAGATCTGTGAATGCAAATGGACCAAAGGATAGATGAGTGGTTAGGGATTTGCATTGATTCTCAACAGGGTGAATGGATATGTCAAAGTGCAGAATACAGTCTGGCAACTGGCCATATTCTATTCTCATTCACTCCATTTGTATACATACATTTCAAACAGAAACTGAGATAAACCATTTTTTACCAGCTACAAATCGAGGCACCCTTTAGTTTCGGATTCTGCACTCACCTGCTGCAGGACCTCGTCCCAAAAGTCGTGCCACGGCTCCGGCACATCGCCCACCATCAGCGACTGCCGCCACTCCGGAGGATTATCATCCCGCCGATGGCGGCGAAGGCCAAAGCGATCGAACTCCACGATGTCGCCGCCGGATGTGGTCACTGCGATATGCAAATCGGTGGCGTTGCTGTAATCGCTGCGGAAATACAGCGCATCAAAAATATATTCCAGCCATAAAAGATACTCCAAGCGGAGCCAAAACCAAAAAATATTGCCCACAGACATTTGCACAAATTTGTTCGGTCCGAATTTTAGAGATGGTTCTGGCTGCCA

TAGAGATGGTTCTGGCTGCCACAAAAACATTGACAAGCCGCTTGAAATAGTCTGATTCGATGGCTTGGCTATTAATATCTGAATTGTTTTAAAATTTCGGATAAGCTATAGAACATGGCCTTTGAATACTGAAGCCTCATTTCATCATTACTTTATACAAAAGTGGTGTATAAATGATTTCGATAGTGCACCACTCCCGATTCAAATGCACATCGAAACTAATAAAAACATTTCGTTGATTTGCTGGTGGACTTGAATGTCACCAGCTGCAGCGAATGGGTTTTTAGAAGGGTTGCACCATACTTACTTGAGGAAATCGCCGGCGGAGGGACGCAGGACAATGGCGCACGGATGCTGCGTGGCCCGCACAAAAGGATATGGCAGGCGGAAGGGCAGGAGGAGGAGGCGTGGCCCGGCCCTGTCTAGTTGCGCACTCGGATCCGCATCCCCATCGACATCCCCATGCGCATCCACATCCGCGTCCAGTGGGGCATTGCAATGGGGACAAGTGTGCGGCAAAGTGAAGCAGAAAACTTTCACGTTGCAGTGATGAAAACAGAGGATGCCCGGATCCGAGGTAATTGTGGTTGTACTTGTCGTAGCCAACATGGCTCTGCAAGTGTGGTTGCCTCTACAGTCGCAAAAAAGGATATCAAAATAGAGAATTCACATTGTTTAGAAGTCATTCACTCAGTTAATGTTTATTTCAAGTCTTCGTAACCCTGCTAACTAAATGAGATCTAAAAATTTTTTGACAAAATAATTTATATAAGATTGTTTACACTATTATAAAAGTGGAAAGTACACTGCACCATTTTATATAAAAACAAAGAAAGGTAATACCTTTAGTTCCTTTTCTCTTTGTGTTAGCTGCAACTCTGGTATCCTGCTTTTCGGCTCCTGGTTACTGGACCCCGGATCCCCTTCCTTTGGCCCCCGGCTGGGGATCCTTCGCCTTGTGCCCGTATTATAATTGATACACTTTGTCTATTGTGCCACTCTCCTCGTCCCCGTGGCCAGCTGCCTTTGGTGCTTGTGGCTTTTGGCCAGGCCTGAAACGAAACGTAACGAAAAATTGTTGTAAATAAGCCGAAACCAGACGAAGTTGCGACCAAAAGC

 

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