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CRIMIC CR00976

Gene Spindly

CG15415

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:3529022..3529041	24A1	FBti0206866	83228

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGACACCCTTAGCGTGGATGACATC	GAGTTCCTTTGCGCTGCGGTTTTTGT

Images

Construct Design Sequences

sgRNA sequence	CAGCGCGGTGTCGACCAAGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCCAGTCGCTGCTTAACTG	GGCATCGCTCGGCAATAAGT	AGGGGCAACTCATACTTGCA	AATCTGAACTGCAAGCGGGA

 

Amplicon 5'

Amplicon 3'

TCCCAGTCGCTGCTTAACTGGGGTACCCTTCATCAAAGGTTTGGCTGGGGTTTCCACGGAATTTAGACCCGTTTCTTCGTTTTCATCTTCTTGTATATTCATTTGAGGTGTTACTCCATCGTTTTTGGCTTTGACTGGAGTGCCTTTCAGCTGCGGTTTTGGAATTAATTCTGGAGCTTCCAACTCCTTTTTGATATCAACCGCGTCCTGCTGTTCCAAGTTTAAGTCGATTTCTCGTTTTATTTTCATACCCATAATTGTCTTAATAGGTGTTCTTTTTATACAAGCCTTGCTTTCGGATACCTCTAGTTCCTGTTCGACTTTTACGGATGTGTCTTTTAGTGCTGGTTTTAATTCTTGGCTAAGTGGCTCCACTACCTCTTCTTTAATGGCAGGCTTTAAGGGCGCATCAAGAACAATAATTTCCGTTGCAGTTGGAAAAGACTTGCTTGTGGTTTCCGCAGCCGATTTCAAAGATGTTTCTGGATGTGTATGTAGTTGAGCTGATGATGATGGAGATTGTGCCGCTACACTGACAATGCGGGGCAATGCAGTTTTAAGTTCCGCCTCCTTGATGTTAAACTCCATGGCCTGCATGGGCTTGAAAACGATCTTATGCTCCGCCAGCAGATTTTCGCGATCAATGAGCACACAGCGGGATTTTAGGGACTCAAAGCGCCAGCTGAAAATATAAATAATTATAAAATAAATATAGCTTTGAAAAGGAATATAGTGTATATACCGTGCCATTTCCTGCTGAACGCATCGCATCCGTTCCTCCAGTCCGGCCTTCTGGATTCGCATGCTATGCAGCTGCGCCTTCAGCTCGTCCGTTTCGCGTCTAAATTGGGATGATTTTTAGGAATTAGGGGTGTGGAGGCTACCTAACTGGTCAGCAAGAACAGGCCGTAGCTGGTGAGGCTCAGCAACTTATTGCCGAGCGATGCC

AGGGGCAACTCATACTTGCAGAAATCCAGCATCGAGTCCAGCCAGGTGACCGACTTCTCGCTGGCCAGCTGGCGCAGCCGCTCCTGGGAGAGATTAAGCTGCCGCTCTAGGCTGTCGTTGGCAGTCATCAGTTGCCGGATGCGCTCATTCAGCTTATCTGGGGGAATACTCCTATATTAATCTTCTTTACAGACCAATGAATGTGGATTACTCACTTTGATAGGTCTTGTCCGCACTGCAAAAGATTGTGCTACAGGCCTGCAGCTCCGTGTGCATGGCCACGTTTTCCCGCTTGAGACGTCTGATCTCAAATTGGCTGTCCGTGAAGATCTTCTTCATATCCAGATAGCTTTTTTTCTGGGCCGCCAGGATCTGCTTCATCGCTTGCCTTTGGTCGTCCACCTCAGCGAACAGAGAGTTGCCCTTGCGGTCTATAAAAATAGTGCATAAAGCCCTTTTTAAAACAAATTTTAGGGTGCCATGTTAATCTTAACTACCATTGTTTTCAGGGGCGGAGGTAAGCAGTGCTATCTTGGCATTGGCATCCACCAGTTGCTCCTGGCAACTCTCCAGCAATTGCAGCTTTTCCTCCAGTTCATCCCGCTTGCAGTTCAGATT

 

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