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CRIMIC CR00977

Gene smog

CG31660

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:4870170..4870195	25B1	FBti0206867	83229

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCGGAAAATGCAGATGGAAATGCG	AAGTTTGGCCACAACTCACCCATGAC

Images

Construct Design Sequences

sgRNA sequence	ATTTAAGTGACCATGCGGCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGGATTCAGTGCTCGTGT	CCACTTCCCCCGACTTGG	ACGTGGCGACCAATATACGT	CCTGATCCGGTGGTATCTGC

 

Amplicon 5'

Amplicon 3'

CCAGGATTCAGTGCTCGTGTGTGTGTGTGTGTGTGTGTGTGGGCCAGACTCTTGGCATAGTTGCTCAAATACACACACACACACACGCGCGCGGGCACATTTGTTTCGGCCATGTTTGCATAAGTATGCATGTTTTATTGCACACACACTCGGTGTGTGTTTTGTGGCTGCTGCCGCTATTTACAAGTGTTTTCCCCATACATTTATACCCTGAAAAAACGAGGGGCGCTAGTGCGAGGGTATGTGTGTAATTGCGGACATTTGGGAAAAAGGATATCAAAAGGATTTGGCGGTAATGAAGACATTCTACTGCTTCCAAGAACTGTGGTGGGATTACTTAAAGTTCAGATAACTCCTGAAGAAAAAACAATGAATAATGGTGTAAAAATAGAAATATTTCCTTACTTAGTTTAATATCTGAAAACACTTACCCAACTACAATATTTCTCTAGCATGCAAGAGTATACCAAATGTTTATGATTATGGCTGCGCGCGAGTGCTAGGAGCACTACATAATGACTTTATCTGGGAGCAGGTCTCGTGCCATTTCTCCAGCTGCCTTATACTGATTGCTTTTGCCTCGATGTGTAAATTTTGGCTCACGTTTGGCAGGAAAAAAAATGAAGAAACACACTCACACACACATGGTAGAGGAGCATCCAGCAAACGTTTGTATATCGATGTTACTTGTTGGCAATTTATTTGGTGGCTTCGGTGGCGTATACGCAATGTGGTCGCTCATCACCGAAAGCCCAGCTAATGGGAAATATTTGTTTTAAAAGCCAAAAGGTGCCGCCAGCCCAACTACATGACTCACGCCCAAACAACGTGTCCTTTTTCCCCGCTACCAGCAACCCCCTCCCTGCTCCCTTCTGTGGTTACTTTTGTTTCCCAAAGCCATAATGATGTCATCAAAGTGGCCCTACCATTTCCTACACTTTGGTGCGTAGATCTCGAGAGTTTCTTGTAACGTGTAAATTATGTATAAACATTTGGTCGGTTAGGAGCTGAACGAACTTGGATTAAAAACCCTTCCCGCAGTCCCACAGCACGGCGAGGGTATTAAATCCTATTATTTCACAGTCGAAACGAAGCAAAACTTTTCGCACTCTTGCCGCAAGGTGTTGCTGTGAAACGTGTCCTGTGCGCCAGGTGAATAATTAATGGTCCCAAGTCGGGGGAAGTGG

ACGTGGCGACCAATATACGTGGCATATACGTAATTTTTTCGCAATTTCCGAAAATATCTTCAATTAGAAAGCACACGTTTTCCGTTTCCTATATAGTGTGGTATAAATAAATTGAGTTTAGCAAAGCGTAAATTTATGCTGTGTTAATTTTATTGAACTTTTTCTTTGTGTATTTAAATCGAATTCGATTTAAATGATATTTATATGCCCATTTCATATACATTGAAATGATTATTTATGACACAACGCAATTAATTGTGATCTGGTTTTATGAATTCAACAGTTTTACGACTACACCGACATCGCCGAGGATGCCGCACGCCAGTTTATTGAATTTTTATCGGGCAAATTCCCAAATGCCAACACACCGATTGCCATCGATGAGCCGACACGTGCGGAGGTGAGTCGCCGGGCCAATGGAATCGCCAGCTACGCCCTCAACGAGGACGACAATCTGCTGGCATTCGCCATTGCCGCGCCCAGCATTCACACGGTGGTCGTTAAATTCAGGGATAACGTTACGGTATGCACTCGCTAATCAGCGATAATGGGGCCATGGGCTAATCGATTTAACTTATTGCAGATACCACCGGATCAGG

 

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