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CRIMIC CR00979

Gene CG5861

CG5861

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:16306902..16306903	35F1	FBti0206869	83231

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGCGGCCATGACGAATATTTCTGGT	TGCTTAAATTCCGATCTGCTGGGCCA

Images

Construct Design Sequences

sgRNA sequence	GCCAAGTCCGCAGCTCTACCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGACAGTCACCGCCAGAA	CAAGTCCGCAGCTCTACCAG	GCAGTCGGCAAATCACCAGT	CGTCGGAGTAATCGGGAGTG

 

Amplicon 5'

Amplicon 3'

GAAGACAGTCACCGCCAGAAGTAGACTAACCAGCGGCTTTAATGCCTTGTTCAAATCGTGACGGGTGAAGAGCCAGATCAGGGTGGCCGTAGTGATGTGCTGCACCAGAAGCACGTTCGATTCCAGACACTTGAGGATGTAGATCCAACTGAACTCGGTGCCTCGGGCACCCACCCACAGCATGATACCTCGGGAAAGAATCACTTCGGCGGTGGCCCCTGCGGAAGTATACGAAAATATTTAGTTTTTACTAAATTTTTGGAAGTTGTCATACATATATGGGTTGGGGTATTAAAGGTTGAGTAGGCATTGGCTCTTGTAACATTAATATATCTGAACAGAGTACTTTTCAATCTTTAAAAGCCACCCTCATCATTAATTAAGTTTAAATAAGTTGGTTCGTTCAACCTACATCCAAGTCCAGCCGTTATGAGCTTTGAGTGGCCCTTGCCGGGTATGCGACTCAAGATTAGGGCGAATCCAAGCAGATCGGCGATGTCCATGCTGCAGCGAAGAATCTCCTGCAGACCAAAGAAACCACAACATGACCTCAATAATTGGGTGAAGGTAAACAAAGATAAGCACACGTACCGCAAAGAAGTTGAACTCGCCGCTGGAGGATGGAGCGTCCGAGTAGAAGAATGTGGCCAGCACCAGCATCTTGACGAGCTGTGTGAATATATAAATGCCTCCCGCCTGCACGCACTTCCAGAAGGCTCCATATTCTGATCTGTGATTAAATAATAAGCCGGGAAAGTTCACAAATAGTTTTTTATTGACCTGGTAGAGCTGCGGACTTG

GCAGTCGGCAAATCACCAGTAACCAGAAGTAAACTCACAGGCCGGAGTATTTATAGGTGAAGTAGTACGGCGTCAGCAGGGCCACACAATTTCCAAAGTGATACAGTGTCATTTTCTTTGATAAATTATGGCTATTTTATTTTGGCTTAATTAAGAGAAGAATTAAAAGTTGAAAAGAAAAAAACTAGCGTTGCTTGCCGTGGGTTATCGATATGTGCTTAACAGGGCTGACGGCATGTCAGGCAGACTATAAAAAACAAGCTAGTTTTGCAGACAATATTTTACAATAAATATTTTAATAATAACTATTATAAATAAATATTTTGAAATATTGGAAAACGTGTGTCTTTCCGAACGAAAAGAAGTAGCAACTGTTTTAATCTGTTTCGTGCCTATCGATAGTTGCTTAAATATCAACACGTCATCGTAATCTATCATCTGGAGGTGTGTTCGTGTGTGTGAGAAGAGTGTGTGTGTGTGCGTTTGAGTGAGTTTTCTGCAACCTTTAGCAATTATTATGAATTTTTTGACAAAACCATTGGGAAATACTTGAACGGAGACCAAATTTTACTTTGTTTAGGACACACAAACCTGCCAGCTAAGTGACGAGCCAAACATCAAACGGTAAACCAGTGCATTAAAGGAGAAAAACAAAGGGGAAATTTTCGCAAAGAAAAGTCCAACAGATCATTTCGTTCGTGTGCATATGAAAAACAGCAACGTCAACTCCCTGGTAAAAGCTGCAATCTGAAATGAGATGCAAACCCGAAGACGCCTTCATCAAACAGACCAGCAGGATTACAGCAGTAGCAGTACCTATACCATTCAAGAGGATCAACAGGGAGGAGCAGGCGCAGGATCTGTGGGCACGGGAACAGCTGGCGGATCGGTAGGACTATTAGCACAGTCCCTGGTTCCACCACCAACTGGTCACGAGGCGGCCATCCACATTGGAGACAACTACCAGTCGGGCGATTCCATTTCCACTCCCGATTACTCCGACG

 

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