CR00980

CRIMIC CR00980

Gene CG4552

CG4552

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:1129665..1129697	21F1	FBti0206870	83232

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AAGCGCAGCTTGTGTTGATTGGCCAA	ATGAGGTTGCAGTCCAGATGGAATGC

Images

Construct Design Sequences

sgRNA sequence	CCAGGTGCTCCGCGTCCCGCTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCTGGCAACTAGAGGTGG	GCACCAAGCTGCTGACTCA	GTGGGTGCTCCGCCTTATC	ATCTCCTCCTTGGACGAGCT

Amplicon 5'	Amplicon 3'
TTGCTGGCAACTAGAGGTGGTCAGCTGTCGATTATCGTAGTTGGCAACACTAATCGATAGGACAGAAAGCGTTATGCATTTCAACTTCAATGTTCAAATGAACAAACTAAAATTTTCTATTCTCTTTTGATACCTGTAATATGAAAATAATATAGATAGTTTGCTTAGATCCCGCTGAATAACCTTATATATATATAACAATATAACTTAAATTTATTTTCTAATTTGTTATCTCAAATTTTAGCATTCTGACAAGTATCAAGTTTGTAATCATTTTTAACGATTTATTACAAATATCTTAATACAATTGTATTGTTAGTTCATATGCACACTGCCATCTCTAACGTGCGCCGTGGCAGACGTAAGCACCTCCCATCGCTATAAACGTAACCATCATAACGTAACCAAAATTCGATCAAAATAAATTTGGAATATCACAAAAATCATTATTCGCACCTGGAACAGAAAGAAAATGCACCAGCCTGGCCTTCTGCAGCTGAATAGATTGCACTAAGGCAGCATTTCGAATCCGAAACGTAGGAACTAGCCCACAGCGAAATCATGGAGGAGAATATGTGGTAAATAATAAACATTGGGTTGGGGCTACGTCAACAACTAAGATTAGTCAGAGAGTCTAGAGATTCAGCTCCAGCACCTCATTTTCTTTGGCATCCATCGCCTTTGGCTTTCTCGAACAGACCACCATTTGTTTCGCTGATTGAGTCAGCAGCTTGGTGC	GTGGGTGCTCCGCCTTATCAGCAGCTGGTTATTTAGACACGTTGACGTCCCCTTCACCCGTAGCATGAGCAGAACAAGTACCAAGCAACTGATTGTGATGGACATCCCTCTCTTGTATGCAGGATCATTGAGCTGGAGTCGGCACTGCTGGACGACTGCACTGTCAACGACATCTACGGCATATGCCAGGGCAAGGCTCTGCCGGAGGCACTGCGTCCGGACGTGTGGCAAGTGTGCCTGGATGTGCGCCACAAATCGGATCAAATGTCGCTCTTCAACGAGATCTACGACCTGCCCTTCCAAAGCCAACTGCGCGAGGATTGCCAGCGGCACGTCGATCGCATGGGCAACGATGAGGAGGACAAGGTCTCGGTGGTATCCGACCTCGAGTCCATCATCACCTTCTACTGCAAGAACAGAAACCTTCAGTATGAGCCGGACAACGGATGGATTGAATTGCTGCTCCCGCTGTTCGCCCTGAAGCTGAATCGCTCCGACACATTCAACCTGTTCGAGTCCATACGAGACACGTACATACCCAAGGGTTGCCGGCCCAAGGGCAATGTCTTTCATGTCTTCCGACTGCTCCTGCTTTACCACGATCCGGAGCTGTGCACGCTGCTGGACACCAAGAAGATCACGCCCGACTTGTACTCGCTGACCTGGTTCCAGTCGCTTTTCGCCTCCTGCAGCAGCCTGTCAGTGATCATTGCCATGTGGGACCTGTACTTTCAGAACGCCGATCCCTTCATGGTCTTCTTCCTGGCGCTAATAATCTTGATCAACGGCAGGGAGCAGATCCTGCAGATGAGGAGCTCGTCCAAGGAGGAGAT

Amplicon 5'

Amplicon 3'

TTGCTGGCAACTAGAGGTGGTCAGCTGTCGATTATCGTAGTTGGCAACACTAATCGATAGGACAGAAAGCGTTATGCATTTCAACTTCAATGTTCAAATGAACAAACTAAAATTTTCTATTCTCTTTTGATACCTGTAATATGAAAATAATATAGATAGTTTGCTTAGATCCCGCTGAATAACCTTATATATATATAACAATATAACTTAAATTTATTTTCTAATTTGTTATCTCAAATTTTAGCATTCTGACAAGTATCAAGTTTGTAATCATTTTTAACGATTTATTACAAATATCTTAATACAATTGTATTGTTAGTTCATATGCACACTGCCATCTCTAACGTGCGCCGTGGCAGACGTAAGCACCTCCCATCGCTATAAACGTAACCATCATAACGTAACCAAAATTCGATCAAAATAAATTTGGAATATCACAAAAATCATTATTCGCACCTGGAACAGAAAGAAAATGCACCAGCCTGGCCTTCTGCAGCTGAATAGATTGCACTAAGGCAGCATTTCGAATCCGAAACGTAGGAACTAGCCCACAGCGAAATCATGGAGGAGAATATGTGGTAAATAATAAACATTGGGTTGGGGCTACGTCAACAACTAAGATTAGTCAGAGAGTCTAGAGATTCAGCTCCAGCACCTCATTTTCTTTGGCATCCATCGCCTTTGGCTTTCTCGAACAGACCACCATTTGTTTCGCTGATTGAGTCAGCAGCTTGGTGC

GTGGGTGCTCCGCCTTATCAGCAGCTGGTTATTTAGACACGTTGACGTCCCCTTCACCCGTAGCATGAGCAGAACAAGTACCAAGCAACTGATTGTGATGGACATCCCTCTCTTGTATGCAGGATCATTGAGCTGGAGTCGGCACTGCTGGACGACTGCACTGTCAACGACATCTACGGCATATGCCAGGGCAAGGCTCTGCCGGAGGCACTGCGTCCGGACGTGTGGCAAGTGTGCCTGGATGTGCGCCACAAATCGGATCAAATGTCGCTCTTCAACGAGATCTACGACCTGCCCTTCCAAAGCCAACTGCGCGAGGATTGCCAGCGGCACGTCGATCGCATGGGCAACGATGAGGAGGACAAGGTCTCGGTGGTATCCGACCTCGAGTCCATCATCACCTTCTACTGCAAGAACAGAAACCTTCAGTATGAGCCGGACAACGGATGGATTGAATTGCTGCTCCCGCTGTTCGCCCTGAAGCTGAATCGCTCCGACACATTCAACCTGTTCGAGTCCATACGAGACACGTACATACCCAAGGGTTGCCGGCCCAAGGGCAATGTCTTTCATGTCTTCCGACTGCTCCTGCTTTACCACGATCCGGAGCTGTGCACGCTGCTGGACACCAAGAAGATCACGCCCGACTTGTACTCGCTGACCTGGTTCCAGTCGCTTTTCGCCTCCTGCAGCAGCCTGTCAGTGATCATTGCCATGTGGGACCTGTACTTTCAGAACGCCGATCCCTTCATGGTCTTCTTCCTGGCGCTAATAATCTTGATCAACGGCAGGGAGCAGATCCTGCAGATGAGGAGCTCGTCCAAGGAGGAGAT

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