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CRIMIC CR00982

Gene l(2)37Cd

CG10563

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:19127862..19127862	37C1	FBti0206930	83234

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTCATCCTTGCGCTTCTCCAGGTA	CTAGTTGGTGAAATCATCGCTCCCGA

Images

Construct Design Sequences

sgRNA sequence	TGGTGCACAGAGCACGAATATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCGGCATCATCAATGTGTC	GCACAGAGCACGAATATGGAC	GCTCTGTGCACCAAGAGAAT	TTGAAGTCCTTGCGGGGATC

 

Amplicon 5'

Amplicon 3'

GGCGGCATCATCAATGTGTCTAAGGTATGGAATTCAACCTGGTCAGGCAATCGGGCAACTTGGCTGATATCTTATTCCATGCACAGGTTTTGGGCGACGAAGTGAAACGATTGGCGTTGCACTTCCATCCGGAGAATCCCTACAACAAACCCACTTTTGGGGACTGCACGGATAAGACTGGTGTCCTGCTATCCATAACCGTGAGGCGCCACAAAAAGGACAAGCAACGGCCTCCGGAATATTTTGTGCGGGTGTTGGGCCACTGCAGCAGGAGCTTCACCTTTGAAAGTGAGTAGATGGTCGGAATTCCATCCACGATATATTCTAATTTCCTTTTACTTTAAGCGCTTTGTGATTTCCAATACCTACCCCTCTGGTCAACTCCCAAAAGTGACAACGCAAATGCTGCCCAGGAGCTCACGTACGCTTTGGACCAGTTGAAACCCAAAAACACCACCGACTTGGACTACTTTAAGTGAGTAACTTGTCCATATTCGTGCTCTGTGC

GCTCTGTGCACCAAGAGAATGAGATCCTGGTTTGAAAGCTATATAGCATGTATAATTAATCATTTTAATGCTCAGTATGTAAAAGCATCGTATTTTTCCCACAGACGTCGCCACAGCCAGTTGCTGGCGCTGCCCGAATTGTTCACCCACGTGGACACTGTCTATGCGGGCACGTATCGCATCGATCCCTCAGAAGACGGCCAACATGAGGTGCTGGGCGTGCAGTCCAAGTCCTCCTACGACAACCAGGGCGTGGTCTCGTTCAACATGGTGGACAGCTTTCCCACCCAGGCAGACTCCCAAAACCTTAAACGACTCAAGGTCAAGTACGTTTCGGATGAGCAGCTGGCATTGGTCAAAAAACTTTTCGATGACTGCCCAATTTGGACCCGAATCGCGCTGCAATATGAATCGGGTTTGACCAACGACAAACTCAAGTGCATCACTCCATCGCTGGCATTTTACTTTAGCAACGGCCCGTGGCGCACTTTGTATGTTCGCTTCGGCTACGATCCCCGCAAGGACTTCAA

 

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