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CRIMIC CR00985

Gene Dh44-R1

CG8422

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:14382935..14382955	50F8	FBti0206874	83237

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGAGTTGATGGATCTTTAAGCCGAG	ACAGTAGAACAGCGACACCGAGAATC

Images

Construct Design Sequences

sgRNA sequence	AAATGCCATACCCAGGGTATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGATCCGCTCCTGGATCTG	TATCGGGGTCACCAGGGAG	TTTTGCCTAACCCCCGACTT	TTAGGCCAGATTCCGAGGGA

 

Amplicon 5'

Amplicon 3'

GTGATCCGCTCCTGGATCTGCACAACCTAGATGGCATCGGTGAGAGCGTGGAGTTGCAGTGCCTGGTGCAGGAGCACATCGAGGCATCCACCTATGGCAACGATAGTGGCCACTGCCTCACCCAGTTCGACTCGATCCTGTGCTGGCCGAGGACAGCTCGCGGTACCCTGGCGGTGCTCCAGTGCATGGACGAGTTGCAGGGAATTCATTACGATAGCAGCAGTGAGTACCCCTTTGACCTTATACTTTAACATTAGACTTATATTTAAACTAAGCGTTTGAAAAAAACTGTTAGCTTTGCACTAAGTTATGAATTTAAATTGGAATTTTTTAAGTCGATGTCCAAACAGAGAAGGCATATTCCCAATATAGATCCAAGTGTATAGGACTAGTCTATTTAAAAACTGTTTGCATTTTTCGCTGCGTGCGTTTTTGTATTTGACCAGAGAATGCAACGAGGTTCTGCCACGCCAATGGAACTTGGGAGAAGTACACCAACTACGATGCCTGCGCCCACCTGCCCGCCCCAGAATCCGTGCCGGAGTTCGAGGTCATCGTGGAGCTGCCCACCATTATCTACTACATTGGATACACCCTCAGCCTGGTATCGCTCTCGCTGGCGCTGATTGTTTTCGCCTACTTCAAGTGAGTGTACCCACCCCAGCCCCCTCCCTGGTGACCCCGATA

TTTTGCCTAACCCCCGACTTACCCCCCGTCCTTTGTATTTGTCTTTGTTCACCAGGGAGCTGCGTTGCCTGCGCAACACCATCCACGCCAACTTGTTCTTCACGTACATCATGTCGGCTTTGTTCTGGATACTCTTGTTATCCGTCCAGGTGAGCATTATGAGACTGCCAGATAATTGCAATTGCTGGCTTATTAAATCATACTCGTGCCTGAAAGGGGTGCTAGTCCAAAGTGAAAGGAAACTATAAACAGACGCTGACAGCTTAAGACTAATGGCTACCACTTAATCTACAGATCTCCATCCGAAGTGGAGTGGGCAGCTGCATTGCTCTGATCACCCTGTTCCACTTCTTCACCCTGACCAACTTCTTTTGGATGCTGGTCGAGGGTTTGTACCTGTACATGCTGGTGGTTAAGACTTTCTCCGGGGACAATTTACGCTTTAATATCTACGCCTCCATAGGCTGGGGTAAGTGTGAATGCCCGATTGTGTGTGCCAGCCAATTAAGTCTTTCTCCTGCTGGCAGGTGGCCCGGCCTTGTTTGTTGTCACCTGGGCTGTGGCCAAGAGTCTGACGGTCACCTACAGCACCCCCGAAAAGGTATGTCTATTTTGGGATTTGAAGAGACCCCTGGAGAGTAGTCTTCCTTTTGTTTCCCATCCGACAGTACGAAATCAACTGCCCCTGGATGCAGGAGACCCATGTGGATTGGATATACCAGGGACCTGTCTGTGCGGTGCTAATAATCAATCTCACATTTCTGCTGCGTATCATGTGGGTGCGTATGGAATTTACACAGAAAAAATATTGATATGCGAAAAAAAGAACATCAGAGAAGATTGAACAAGTTAACATGGATGCAAAAACTCACATTTATGAATCACCCAATTAGTTTCTTGAAACACTTTAAAGCTATTGTATGAATTTACAATTTATCATCCGACATTTTTCCGACTGTAAATTGCCGAAAATACTGAGCCAGATTATCGGGTTGCTTCTCTTTTAGGTTCTAATCACAAAGCTGCGCTCGGCCAATACAGTGGAGACTCGTCAGTATAGGAAAGCTGCTAAGGCACTGCTGGTGCTAATCCCACTTTTTGGCATCACCTACCTGGTGGTGCTGGCTGGTCCCTCGGAATCTGGCCTAA

 

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