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CRIMIC CR00993

Gene jub

CG11063

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:13828156..13828170	12C6	FBti0206877	83240

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAATCAAGGATCTGCATCGTTGGG	AGAGTGGGATAGAAACGAATGGGGTG

Images

Construct Design Sequences

sgRNA sequence	GCGATGAGTGATGACACGAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCTCGCAATAGACCCGAC	ATCATAAACCGGTTGGCGGG	ATGACACGAACGGAACCCAA	CAACTCCTCAGGCAGTTCGT

 

Amplicon 5'

Amplicon 3'

CTCCTCGCAATAGACCCGACCATGCACATTGTAGAACGCCTTGCCTCGCAGCGCCCGTCCGCAGGAGCAGCAGATGAAGCAGTTGGTGTGATACAGATTGCCCATCGCCTGGCAAGCCTGCCCGGCACCCTTCACCTTCTCGCCGCATGTGTGACAAATGCCTAGTGATTGTAACCGATATTATTGTGAGTCAGAGATATTATGGTTAAGTGCTAACAATAAATACACACCAAAGTATTCGCCGTGCTCCTCGCTCTGTTCGATCTCCTCCTCCAGCTGGCGTGTGAGCTCTTCGATCTTGCGCTGCGCCTCCGTCGGGCCAGCCGGACGCGGCGGGGTGACGCTATACGGTAGCAGATTCTTGGTGAGACCTGATGGCGGTACAGAGTAGATATTAGGTCATGCTATTGACTTGGCTTGCAAGGCCAAGTGAAGATTAGTCAGCTTAGTTAGGAAAAAACAAAAATAAATATGCGGGCGCATTGTTGGGACAAACGAAAATAATTGAAATAAATAAATCAGGAAAAAAGCTGAAAAATTCGCGGTGTAAGCCAAATTCTTGAATGAAAAATGAAATCAAATTATAATAATATTTTTCTAATTATAATTCCCATCGACAAAACATAAAAAATTTGTTTGTTTAAACGATAAAATCGCAATTTCAATCATGGTGAAATGAAAATGAACGATATTGCATTCGAATCGGTTTCGGTTGTCATTGAATCGGTTCAGGTAAAGGACTTAAAATTAAACCGGTTAGCGGCAACCGTTTGACCAAAGTTTGTCGCCCCGCCAACCGGTTTATGAT

ATGACACGAACGGAACCCAACCCCTTTGGAGAAACCAAGCTCAGCCCGTCATACCTCTCCTGGGTTTCAAGGCAGCATTAACGGCTGCGTGCAAGCATTTTTGATGGGGGAAGCATTTGGATTCATTAGTTTGATTAATACAATCTCGAATTGATGGTTGTTTAGTGATGGAATGTATCCATGTAGGCAAAAGTATGCATACATAGATACTGAAATATTATAATATGTTGCATGCTAGGCGAAATACATGTTTATGTATAATAAAAAGTACGTTGAAGGTGCTTTTTATTATTATTCCATATTTAAATGATAGCTTTAGTTTAGTAAAGTAACCATGCATCTTTAAATGGATCGATAGAATTTTTGTTCACTTTACTATATATAGCATGTACTCCACCTAAGCGATGCAGTATCTATTCCATCACTTAAAGTAATTCGCCATTTTGGGGACAGACGATCGTCAGTCGCTTACCTGTGCTGCCCTGCGAGGGAGTGGGTGATGTGGCCACCGCCAGAGGAGCCGTCATCGCAATGGGCGTCGCCCGCAAGGAAGCGGTGGATGTTGGTGCTGCTGGTGCCGCTGCTGCTGATGTCGGTGCATTGGATGTGGTTGGTGGAGCTGCCGTGGCCATGCCCATGGCCAGTGATGACGTTATTATTGGTGGTTCCTGGACAGTTCGCTGGGCAGGGGCTCCCGTTGCAGCCGCCCTGCCGGTAGCCAATCCTGCTGCCGTATTCCTGTGCAGATTCGCCGTCATGCAAACGTAGTCAGAGCTATTGATGTCCTCTATATAGTTAGGATTAACGGAGACATTAATGGCGCCGCCGGGCGACTTAACGGGCGTGTGCTGCTGCAGTTTGGCCACCGACTGCGCGGTGGGTACGGGACTTAGGTGGCTAGGTAACGATAGATTCCTATATCGTTGCGTTGGCGATCCCAAGGAGCTAGAACTGGCCGTGTTGCTGCTAATGCTTCGCTGGTGCATGGGCGAGGCCAGCAGCGCATTGAGATCCAGACCGGCGGTGGACGAACTGCCTGAGGAGTTG

 

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