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CRIMIC CR00995

Gene mats

CG13852

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:22421738..22421738	94A12	FBti0206931	83241

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCAGGAACCACTCCAAAGTGACTG	GTTGTTTCTCGAAAGCGGGAGGTGTT

Images

Construct Design Sequences

sgRNA sequence	ATCCCCAACCTCTTGTACAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATCAGTCTGATGCGGTGGC	CAAGAGGTTGGGGATATCACC	CCCAACCTCTTGTACAGTGGA	GCCCGCTATAGTTCGGTTCA

 

Amplicon 5'

Amplicon 3'

TATCAGTCTGATGCGGTGGCAGACACATGTGGTTTTGGGTGCTCCGCGTGTGTAGGTTAGTTTTCGCAGGGAAAACATTTTCATTTCACGGGCAACCCGCAGAAAATTCAAATATATGATTATTGCACTGAAATATTTACCATGTCTATGCCGAATATATGCCGATTTAATTAAGAAATATATATTTTTATACACCCACGATGCTGCTACAGTTGTTTTCCCACGTGCTTACTTCAAGTGTGACCATAGCAAGCCGATTGCGAAATGTTAGGGACATACCAGATAAAATACCGCAGTACCGTACTGAATTATTTTAACAGGCTGGCCACGTTGATAGCCTGATAACCTGTCGCGTTGTCCCTCTCGCACTTTCATCAACTGTCTCTGTGTGTGAAAGCAATTTTATTTTTATTTATAACAAATTTTTTGCGTTTATAAACACAACAGAATTAACTAGTTGGCTCGGCACACTTCCTGGAACCGCTCGCATCAGCTGTTGGGCTACGGCACTAGTTAACAGACCGACCAACTGGAGGCCAGATACAGATACCATCATTGTCATTTCCCAATTGACCAGAGAAAGAAACCTGCTGCGAATTCCAGTCACTTTTCTTTAATTATTATTCGGCGCTAATTGTGTAAAAATCGGCGGAATAAAGCTGCTGACACGACACAGAAGAGCCGAAAAAGCGGTGTGGCACTCGCCGGAAAAGTCTACGCATAGGAAAATTCCATTGGAGGCTCAGTGAAAGGCGAAGCACAGCAATTGCATGCCATGGACTTCTTGTTGTAAGTTCTCAGGGGGGCAAAACGTAGTGCAACTGCAATCCTTTCGAGCACAAAGAAAGCTTGGATCACCCCGCCCCACATCTGACTTTCCGGCTTTGTTTGCCCTTTTTGGGGTGATATCCCCAACCTCTTG

CCCAACCTCTTGTACAGTGGAACTTCCCTAATTTTGTCTGCGGTGTTTAAAAAATAGCTTTTCAAAGATATCTTGCATTCTTCCTCAGTGAATTTAAAAAAAGAAAAAAAAAGGAAATATTTCTTTTAGGGAGTGTAAATTGAACGAAGTCGCTTACGTTTCAATCTATAGAATCTTTACTGTATTATGTTTTACTGAGTGGGGCTGTTTGGGGCCGCGACTTATTGCCTTTCTCTTTCCACAGCGGTTCCCGTTCCAGCAAGACCTTCAAGCCCAAGAAGAACATCCCGGAAGGCACACACCAATATGATCTCATGAAGCATGCGGCAGCCACGCTGGGATCGGGAAACCTGCGCAATGCAGTGGCCCTTCCAGATGGCGAGGATCTCAACGAGTGGGTGGCCGTTAACAGTGAGTCTAGTATAGTGTAACTAGCGAAAGATAACCAATTCTTATTGCCACTCCTTCGCAGCCGTGGACTTTTTCAACCAGATCAACATGCTGTACGGCACCATCACCGAGTTCTGCACCGAGGAGACCTGTGGCATCATGTCGGCTGGTCCGAAATACGAGTACCACTGGGCCGACGGTTTGACCGTAAAGAAGCCCATCAAGTGCAGCGCGCCCAAGTATATTGACTATCTGATGACTTGGGTGCAGGATCAGCTGGACGACGAGACCCTGTTTCCCTCCAAGATCGGCGTGCCGTTTCCAAAGAACTTTCACTCCTCCGCCAAGACCATACTGAAGCGTCTGTTCCGCGTGTATGCGCACATCTACCACCAGCACTTCACAGAGGTGGTGACGCTTGGCGAGGAGGCCCATCTCAACACGTCCTTCAAGCACTTCATCTTCTTCGTGCAGGAGTTCAATCTGATTGAGCGGCGCGAACTGGCCCCGCTGCAGGAGCTCATCGACAAGCTGACGGCTAAGGATGAGCGGCAGATATAGGAATCACTGCATGGAGCCGCGAATTTTCGCGATTCCGGCGGCGGTGGTGGTGGTCTGCGCTTGCAGTTGGACTTGTGATGAAAGCGCTCTGGCAGACTCGTGTATTACGCTTTAGTTAGTTACCGCCAATACTTTTATGTTTCGTTCATTGCTTCATGGGGTGCCTCCCACTAAACAAAAGAAAACAAAAAACCGCCAATTGGCAATGTGAACCGAACTATAGCGGGC

 

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