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CRIMIC CR00996

Gene dpr19

CG13140

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:10377461..10377461	31D11	FBti0206932	83242

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGAAAGCACCCCACCAAAGTTCGTT	TAAAACTGGCCACTCTGCGGATTGCT

Images

Construct Design Sequences

sgRNA sequence	CTAAGTTGTATGCTATAAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGACGATTGGCACTTCGAA	AGCATACAACTTAGCGCAAAAGT	TTTGCGCTAAGTTGTATGCT	CGCTTGTCACTACTGTGGGT

 

Amplicon 5'

Amplicon 3'

TCGACGATTGGCACTTCGAAAACGAAGTTGTATTCGCCGGGGTGTGAACTGCGGACTCTTGGCTCCAACCAAGTAAATTCGAATCCAGAATTGTGCCATTTTCTCTAAACACACACACACACACACACACCCGCACGCACATACACACACCCGCACACACAGAGGCCGCGTGTGTGAAAATATAACGAAAATGCAGTGAGAAATCTGCACTTCTGACGGTCAATACATACAATTTATACATCTTTCACCTGTGTATCTGTATCTGTATTCTCGAAAATATGTAGCTATGAAATAGTCATACGAATGCTTTATGATTGCATAAACAATTAGAGGCGAAATGTGTATGTACGCGTAAGGATTTGCCGAGCAGTCAGTGCAAAAAGAGCAGAAAAACGGGAAAAAAGTGAGTTTTCCTCCATCAATTTCTGTGTATGCATTTCCTCCAAAATATATACATATATCTGTATGCATATATGTATGTTTGTATCCGTGACATGTGTTTTTTTTCTGTGCATAAAATATTAGTGAAGAAAGAAATTGAATTCCTCCACTTAAGTCAAGATTATCCCAAAAAAGAAAAAACATGATACAAGTTTCAAGTTGTTTGCGTGACCCATACATATAAATATCTTGTATCTGCGAGCTATAAAACATACATACATACTCCACGGTGCATCGTTGTTGCCACAAATATTTTTAGAAATACACATTGGACATACATACATACATAAATACAAATGTACTCATAAACTGAAGTTCGTTGCAGGAGGACAATGTTGCTGCGGGTATAAAGTTGAATTAGAAAAGTATACGATCCGCGGAGCCTCGAATTCGGAAATAATGGAACCGAAAAGATGGCACTTGCATTTATCATGTTTTTTGCTACTCCTTAGTTCAACTTTTTCTGGTAAGCAAAGGTCGCCAACTTTATGAATCGTTAAACGATTTTTATCACTTTTGCGCTAAGTTGTATGCT

TTTGCGCTAAGTTGTATGCTATAAATTGGAAGCGAAAACCTTCGATTGCGTTTTTACTTTATTTTGATTGCTTTTGAAGGTAAATAAAAAAAAATATTAAATACCAAGAGAAATTAGTGAACGAAAAATTTTATTTCTATGCAAAAGTAATTAAAAATAAAACGAAAGCTATGAAAACGAAAGCTATGAAGGCATAGATAAAAGATACATTTTTAAGTTTCATTTTTACATTTTACATTTTTAAGTTTTTTTTTTTTAACATTTCACCGCAAATCACTTTGGATTCATTCATTTCATGTGATTTTTGACACATTTATATTAAGAGATTTTGTTTAATATTCATTGAACAACCATTTCAGATGTCGGGAAGATCACTTCGTCTCAAAATCATTTTGGTAACACCTTACAAAGTCAATTTAATACGAAGAACAACACTCGAGTTATTGCACAAAAGGGAGGACTTGCCATTCTGCCCTGTGTGGTCAAAGTTAACTCCCCAGCCACGGTGAGATATATGTTTTTCTTAAAATTTATACATCGATTCTAATACTTAGACCTTTTGCAACATTTCAGGTTTCCTGGATACGTAGAAAGGACTTCCAGCTCCTGACAGTGGGCCTATCCACCCACAGTAGTGACAAGCG

 

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