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CRIMIC CR00997

Gene DIP-iota

CG11320

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6621131..6621131	26F2	FBti0206933	83243

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCTGACTCAGAGGCCAGAATGAAGA	AACATTCCGAAGAAAGGGACGAGCGA

Images

Construct Design Sequences

sgRNA sequence	CGGAGTGGTAGCGTATAAAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTGGAATCGCTTGAATCG	TAAAGCCGGAGTGGTAGCGT	TATACGCTACCACTCCGGCT	AGGGATATGGGGGATACGGG

 

Amplicon 5'

Amplicon 3'

CGCTGGAATCGCTTGAATCGCTCGGCCAGGACTTAAGCCGCATTTTCCCCGCGAAGTCGCTGCAAATCCGGCCGTGTCCTTTCCCCACGCCACTTGAACTTGAAACGGAAAGTGAATGTCCTGTCTCCTGTTAGACACTCCAGCTGTCTGCCGGCGAAAGGAATCACAATCACAATCAGCACGCAATGTCTGCCAAAAAGTTAAGTAAAAACTGCGAAATAACCCACTGCAGTGGCAGTTCCACACCCGCAACCGCCAACCGAAAAACGCGGCACTTCAGCGGCAGAAACCGCCGGCCAGGAATAGAAAAAAAAATGAAACGAAAATATCGCAGAGTGCAATAGGAAGTGGGAAAACGTCGCCTTTCCGCGACTGTCTGTCTGCCTGCCCGCTGTGCGGTGGGTGGTGGGTGGTGGGAGTTGGTTGGTTGGCACGGAACATCGTGCGGTGCGGTGCATCATCACTCCTTTGAGCCACTTCTCACTTTCTTATCGCACGCTGCGGCTAGCCTGCCAATATTTATGTTTACTTTGCATCTGGCACATTGATTTAAATGCGAATTTTGTCGAATTTTGATATATTTATCGCATTTGGCTCGCCAAAGTCAAGGAAAATTTGTTTTGTTTTTTTTATTCGGCGCACATTTTCCCCAGCGGAAGTTACCCCTCGTTACTCTTTCGCAGCTCTCCACCGGAAACCCCTCTGCCCGCTCCCTTTCCCTATCACCGCCATCCTGTCTGTCCCGTCTGGGGAATATGTTGGCCACAGTTTATTCACTTATGGACTCGTTGGACTTCCGATTACCAGCTACCTTCCATTTTATACGCTACCACTCCGGCTTTA

TATACGCTACCACTCCGGCTTTAGATAGTTTGCAGTCAATTAGCTGTACACTTTTGGGTTCATCATTTCGTGTTGGCGGGTTACAAAAGTTCAGCTTGTAAACAGGATACTTTTTAATTGTACAACATTCAAAAAACGTCATATTAATCGAGAAAAACTATCCTTAAAATGTTTAATTTAATATTTAATGGGTTATGCATTTCATTCAAAGTAAATCTTATATTTCTGACTAATTTCATTATTAGCAATCAGATTTATTTATCCTTTAGTTAATCACATCCTCGATCCACTTACAATTGTTTAGCTCCGAAAAGCTGGCCTGGCTGAAACACACGCTCTGCAGGAGCAGCAGCCAAAAGCTGGGCAATCGCCTTCCCATAGCCGAAAACTCAACCGCAATCAAGGTGCGAAACTTTTGAATATTTGCTGATTTCCCACTTTAATTTTTAACTCTGCCACTTTCACTGCCCAAATGGCCGAAGTTCAACTTCCGCCGCAACAGCGCAAATGTATTTTTTCCGCTTCTGCGGATGAAGTTTTTTCCGCCGATTGCCGTGGCCAAACGTCCGCACACCCGTACAACTGGTCGCGAACTGATAATCAAGTGTCAAAACTCATATTCAAAATTCAAATGCGGCACTGGCGTTGCGCATGTGTCCTGCGGCCTTGCGAAGGTAGTGGGTGGTGGTTGGGTGGTTCTAGTGTGTCTAGTGGGTATGGTTGTAGCCCAGTAGTAGTCAAGGTGCCCGGCAGCCGAAAGACGGTGCTGTGCAATAGTAAATGTTTCATTCATAAACCGCCCGACGTAATATTTTCATATGCACGTGACGATATGAAGTATACGACATGTTGCCGGGGGAAAGTGACACATTATTGGTGATTTTTTTGTGGGCGGCGGAGAATAAAGGCCAACTCCAATACCATTCTAATTAACCCCGAAATGCCGCGCATCTTCTGCCCAACTTTCACCCGTATCCCCCATATCCCT

 

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