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CRIMIC CR00999

Gene Cam

CG8472

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12265343..12265321	48F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGCAACATTAGACCCTGAGAATCG	GGAAGTGCCACTTTCGAGTTGCAACA

Images

Construct Design Sequences

sgRNA sequence	TCTCGGGATCGGGTTAGAAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGGTTCTTTGGAGCTTCCC	AGACATCTCGGGATCGGGTT	AACCCGATCCCGAGATGTCT	TCGTTTGTCCAAACCCCACA

 

Amplicon 5'

Amplicon 3'

TCGGTTCTTTGGAGCTTCCCCCCTACGGGGTCTTTCTTGATTATTCAGTCGCCGCTGACGTCAGGTTTTGTTGATTCAGCGGAAACTTAACACCTTTTTCAAGACACCAGATATATATATATATATGTAGAAATATTATAAACATACCTAGATACAGATACAATTGTATCTGGACTTGCCTGGCGACGGGCGGGCAAACAATACGCAAAATTATTCAAATTAAACACAATTGTTCTCTCCCTTCTCGTCTTTTGTTATTCCCGATTGTGTTCACTTGGGTAGTTTTTTGTGTTGACATTGAAATTCCATCGAGATGCCGTTGAAAAGTAATTCACAAAACAAACCAGGTCGACGTTTAGTCCTATATCTATCAGTAAAATTTATTTTGTTAAGAATATAAAGTCGAAAGTTACACAACGTTTATCATAATGGTGTGTGTTCGATTCATTTTTGCTGCGTTTCATTCAATTAATGCTCTATTCATTTGTCCCTTTGCAGGCCGATCAGCTGACAGAGGAACAGATCGCCGAGTTCAAAGAGGCATTCTCGCTATTCGACAAAGACGGCGATGGCACCATCACAACAAAGGAGTTGGGCACAGTTATGCGCTCCCTGGGCCAGAATCCCACAGAGGCCGAGCTGCAGGACATGATCAACGAGGTTGATGCCGACGGTAAGTTGTGGAGGATTGTGGGGGGAAATACTCTATAAAGTGGGATAGCCTTTTGTGGGGGTAATGTACGTGATTGAAATCCTTCATTGGGGTGATGTAATAAATTTCAAGTCAGATTCACATTTCTCATGCCCTCTGAGCATTTAAAACTTACGAATTCGCGAAAGAGATTGCAAAATGCATACTAATCTGCAAATGTTAGCTAAAGGGTTCAAAAATCGTAAATAATCTGGTTTTGTATGCCTCGTAACTTCAGTATTTCAAACAAATTGTGAATTTGCCTTAATTTTCTGTGACCCACAACATCTAGACTATGAAGTCAGACTTCGTCAGAGCTTACGACCAATGTGCAAATGAGCTGATTTTCTATGCTATGCTGGCTATATATGAGAGCTGGCTAGAACCCCTTCTAACCCGATCCCGAGATGTCT

AACCCGATCCCGAGATGTCTAATTTTGTTTACCCCAATCTATTTGGTGTTCTGGGCCTAATCCCTCGCACTCGTTCCATTCCCCAACTACCTCTTTCGCAGACAGACATAGATCAGACCAACATTTGTACGACAAACAATGGGTGGAAAAAAAAAAAGAAAAGGAAAATCACTTCTTGGCTAAAAATATGCAACAAAAACACGTCATAGGCATTAAAAATAGATGACTAAAATATTTTGGCTCTTTCCCGTGTCTTGTCTTTGGCAGCCGCTGCTGTCAGATTTTTTTTCGTTGGTATTTCATTTTTTTTTTTTAATGTTTATTTTTGAGCAAAGGTGGTTGTTCGCTTAGCTCGAAATCTCGTACAAGTTTTAGTTTAACAAACTTTCTTGGGGAAACGAGGAAGATCCAATTTTATTTTCGATGCAAGAGGAAATCGAGCGACACACGCCTCGCGCCAAAAATGTGGCAAAATGCGAAAATTTGAATCGTAAAGGGGTTTTTCACCTTTTGCCACAAATAAATAGATTTTCCAATTAAGTAAGCAGGTTTGTGCTTTGCTGCTTTTGCTAAGGATAATACTTAGCAACTAAATCATAAGAACTCAGTGGCTGGCACAAAGTAACTTTTATGCTCATCATCACTTGTTCTTTCATAAGTTTTGCAAGTGTCCCCGCCCAATTTGTATTTGTTTTCTCGCTGCTGCTCCATTTTCTTTGCCAGCTTTTCTCGGCTGCCTGTTGTTGCTGCTGCTGCTGCTGTTGCGCTTGCACGTGGAAATTCTGTGCGCATGTTGTCGAAAATATCCAGGACACAATCTGCAAACTCGGAGTCCTGCACACAGAATTTTGAGCGCAGGCGCTGTGAAAAACCCCTGCATGATTTTTCTCTTTTTTTTTTTTTTTTTTGCTGGAGCTAAAGTGTTGCAATCATGGACAAGTGGGTGATGTGGGGTTTGGACAAACGA

 

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