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CRIMIC CR01004

Gene dpr18

CG14948

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:16066209..16066209	14A6	FBti0206934	83245

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCCCATTAAACTGGGTCACACAGAT	ACGTAGGGGTTGCCGCATTCATTTTG

Images

Construct Design Sequences

sgRNA sequence	CCTTGGTTACGTTGTTAAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTCCCGAAGCTCAAGCATT	ACAACCTTGGTTACGTTGTT	GGTTGTAATTAGTCCTAAGCCCA	AAAACATAATACTGTTTGCCGGA

 

Amplicon 5'

Amplicon 3'

TGTCCCGAAGCTCAAGCATTAGGTCACTTTTTCCCAAACTTTTAAATGACTAACATAAAACACTGCAGACAAACCAAAGTTCAAAGTAATTTGTTTGCATTTATTATAATAGTTGTAAGAGTAAGAACAAAGAAATCATTTTCCCTGCAATTAATTCACTTATTCTTGATATGCATCAGCATCCTTGTGGGTAGAAACTCTTGAGCAAATGATTTTCCTTGAATACTTTACTACGTTCTCTTGCAGTAATTTAAATGGTAAAGAAAAGAGGGAAATATACGCTGAGCCATCGACAAGATTCAGTGCCAGAAAGCAGCTGAGTTTTCTCGGAACTAAGATGAAGGTTACTGATTTCTCTCGAGCACATTCCGTAAGTCACGTCGCCCCGTGCAAAGGATGAGGCTTCCGAAAGGATATAGGACACTATCACCAGCATCGAACGCTGCCCAGACGCTACCACTCTCCTACAATTCACAAACCAGTCTGAGCCTTCTTCGGCTGCTCACAATTGCTCTGGTGATTGGATCGCAAGTTCAAGTTCTATGTAAGTGTAGCCCTTAAGTTTCCCTTAACTTTGTTTGCAAAACAGCATTTAAATAACCCCGAAGCCTTTGTTATGACTCCTTTTCCCATGTTTTGTGCTCTTTACTTATCAAGGTAGCATCAGTTAGGGGAAGTTCAATTATTCTTAATGGACCGGGTTTTCTTAGAAACTTACAACGGATATTTAAACATCAAACTTTCTAATTCGGACAATTTGGTAGAAAACTGGGTATATAATACACTTTTCATTCATTTGCGGCTAGTAAAATCTGAAATATTTTGTCATGGGAAGCAAATATTGAAAATATGTGTGTTAAGTTAAATGGAAAAAAGAAAATAAGAAACTATACCATAAAGTCTTGGGCATCCAACGAATAAAACTTTGTAAAAAGTTGTGGCTTAAAAATAATACTACAGATTTACGTCTATAACTACAGAGTACGAATTTAATATTATTCAGTATATAGTTAGGACCTTTATTTCTACCAACCCTACTATATTTTTACCTATTTAACAACGTAACCAAGGTTGT

GGTTGTAATTAGTCCTAAGCCCATGAATATAGCATATTTGAATAAAGCCAATGGGGTAATTTAAAAATGGTTAGATATTTCTGAGGAGCTGAGCTCTTGTTTAAATATATTTTTTCTTGCATTCTTCACCTTTTCAATTCAGACACGACATCCGTCGAAACAAAATCCCCCTCCGCAAGTGAACAATCCAGAAACGGCGAGAATCGTTCATTACTTTTTGATGACTATAATAAAACTAAATCGACATTATCAACTGCTGATTACCTGTCAGCCACAAGGGCAACCCTTTACGCGTTTTCATCGCAGGATCAGGACGAGGACCATTCCCAAATGCCAATCGAGGCGTCTAACGCTCCGCACAGCCCCATTCCCACGAAAATGAGTCGGGGTAAAATACCCATGGAGACACCGGGCAGCATGGAGTTTAGCAGCAATAGCCTTCCAATCAAAAATGTAGGGACAACTGATCAGCTGACGACTGTGACCATGCCCACCACAGCATTCGCATCCCTAAAAGTCGACAGAAGTACCATGAAACAGCCAATTGATTCCACACGCACGAGGAATCATTGGACAGCAAGTGGTAAGTGGGATAGCTTTGGTATTATGCAGAACAGCATATAGCAATTGTACAAAAGTACGTGTATGAAAGTTTTTAGCAAAGTTTTCATCGTAAATATTGCATTTTTGGCAACAGCCATTTGATCGAAACAAAATTAATAAATGTCTAAACATGGATTGCCATAACACGTCAGACTCGTTATGAAATTTCAAATCGACTGGCATTCAAGTTTAAAAATTAAATTTTTTGACTTTTTTTTGGTAATTTTGATAAATTTTCCGAAATTTTTTTCTAAATCCGGCAAACAGTATTATGTTTT

 

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