• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01005

Gene CG9911

CG9911

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:16295984..16295984	14B9	FBti0206935	83246

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGCAGTTCCACAAGGTGCGCAATA	GCTTGGCATGACTTGGTGCGTTTGAT

Images

Construct Design Sequences

sgRNA sequence	TTCCCGCTGCCAGCGTGAGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGGTCCCTACGATCGAAG	ATGCCATTCCCGCTGCC	GGCATGGGGATGACTGTGAT	ATTGAAAACGCGCTTGGCTT

 

Amplicon 5'

Amplicon 3'

GCTGGTCCCTACGATCGAAGTAGCCCAGAATGAGGCGCTTCTTCGAGTCCAGGTTCTCCAGATCCTTCAGGGATTTGAACTCCTGAATGGGATCCTCCAGCTGCTTCTTGACGAACTCGAGGAACGCTTCGGCTGAACGCTGTCCTCGGTATTCGCGCTTGCTGAGCTGCCCATTGCGTACAATTTTCAATGTTGGATACTTGTTTATGTGGAAACGGGACGCGATGGCCGTTTCCTTGTCGCAATCGACCTTGCCTAGCACCACTTTTCCAGCCTCTGGAAACTCCTCTTTAATCTGTAAGAGAAATTCGATTGAATGCCACGTATTTATTCGATATAAAATGTATATGAATGTATCGCATTGTTTGTAAGCTTTTACTGGCGATCCATTTGACTCACCTTATCTGCAGCTTCCGCGAATATAGGTGCTAAAATATTACTAAACCGACACCACTCCGCATAAAAATTAAGGAAAACCAATTCATTAGACGCTGCAAGAAAACAAAACGTGTTTTTAATGTAGGCACCAAATTGTGTGTAAAATTGTTGATGCGTAGAATACAACTCCGTTCAGATACTAAATATAATAACTTTTAAAAACATATATTTGGTAATATATTTATATCAATATTGAATTACGGGTTTATGTATTAATAATTTAAATGCATATGTATTTTTATGGAATATATTTAAATTTGTCGCTGAATATACATATTAAAGTCAAGAAATTTCTAGCATCCTACTATTCCATAACTTCCGAAAGTTAAAATTTAAGGGGTGCAAGCCTAAGGCCAGGCTTTACTGTAAAAGTTAACGTTTGGATAATAAAAAAAAATAACTTTAAAAAAACAATATAGTATTACCGCAGGATTGTGTACAGGACACACCGTATAAAAAGGACCTATTCATGCAAAGACCAAACTTCGGACATTCTGAGCAAAGAACTGCCGACCAATGGCTAGTGCCCATTTTGGCGGACTATCGGACCGATGCAGCATGAAATATAAACCGCCCTAATTAAGTTTTATCCAAATTCCACACCTTGTCCTTTACGAAAGCGCATAAAACTTATTTTACCACGTTGGCAGTGCCGAATTGAGCGAGCCAGTGATTTTGTTTAGTGACACGTCAATGGGACAAGTCGGCTAATTGATTCCCGCTCACGCTGGCAGCGGGAATGGCAT

GGCATGGGGATGACTGTGATGGTTATGGTATGGGCATATCTCACCTAGGGTCATATCGATATTATCACTGGTCATGGGCACGGCTCCGGCGGCATCGGCCGGCTGTGTGTACTGGAGTAGCTGTAGAATCTGTAAATTCCATAGGATGTCAGTTCTGGTTAGTTAGTTATTTGGTTAGGGGGAGCCAAAAAAAAGCCGGGGCTGTCTGGCCGGCCCCTCCCCACTGTTTCCCCGCCCGCTGATCGAGCGGGTGAGCCACTCCAGTGACCAGCAACATGACCAGACAGAACGGAACACGTCGACTCACCGCAACTAGGGCCACACACGGCCCAAGCTTTCCGATTCCGATTCTGGCTCCAAACATTCTTGTTCGGCTTCCGAATGGCGAATGCGGCTGGCGAGGAGTGGGCGTAGAATTGCGTGCACCGGCGGAGAGTCCAAGCGATCGGTTCTCTTTTTTTCTGATTTCAGTTACACATCAGCTGGAATGGAATGCAAACGTACGCACAATTATTAAGTTTTACAAATTTTTGGTTGATTTTATCGGCTGGCCAGTGTTGGTTAGTCTCGAATGCACATCTCATCGGCAATCGAGTAACGATAGCTTAGTCACATGACTTGGCCATCGCTAACAGCAGCGATAACAGCGATAGCAGCAAACAACGATAGCAATTTCAATGGAAATGAAACAAAAAGTGTCTAAATGAATACATATGCATACATATGTATGTGCAACAACATTTGCAAATCTTTGTTTATAATAGCTGACGCTTATGGATTTTATGGTGGTAAATAGAGCGAGAAAATCTAGAAGCCAAGCGCGTTTTCAAT

 

---

Search Again