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CRIMIC CR01007

Gene sob

CG3242

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:3578826..3578826	24A1	FBti0206936	83247

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGAGAACTCAAGGCAAGCGCAACA	CCTTGTTGGCACTCGAGCGATAAACA

Images

Construct Design Sequences

sgRNA sequence	ATAGCGGATAAGCTACTTCATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGACGGCCACCAAATCTCT	GCGGATAAGCTACTTCATGGT	ATCCGCTATTTTTGACCTACCTA	AACAACATCCAAGTGCGTGC

 

Amplicon 5'

Amplicon 3'

CTGACGGCCACCAAATCTCTGGAGGATGCTGCAGTACTGGCGGAGCCCGTGGGCAAGCCCAACTCCGAGCTGGAGCTGGAGCCGGAGCCAAATGGATCACTTAGGTTGCCACCCACCACACCGCCCACTCCGGCGGACAAGTTATGCGTCAGGCTGTGACGTCGCAAGTCGCAGTTCCGCCGGAAGACTTTCCCGCAGGAGGCGCAGTTGTACGGCTTGATGTCGGTGTGGGTGAGCAGGTGGGTCTTCAGATTGGAACGCTGGTTGAAGGAGCGATTGCACACGGGGCACTTGTGCGGCGACTCCTCCATGTGCAGGATCTTGTGGACAGCCAGAGTGCGGGACTGACAGAATCCCTTGCCGCACTCCGCGCACTTGAAGGGCTTCTCCTTGGAGTGGATATATCTGCGGGTTACCAAAATGAATGAGGAGAATGGTGGGTTAGATTTTTATGGAAACCAAATGCTAGTCTTTTGTGACAGAGTGATTCTCAAGAGAAGGATCATTTCTTATTATACCATGAAGTAGCTTATCCGC

ATCCGCTATTTTTGACCTACCTATGATCCCTCAGATGATCCTGCCTCCTGAAGGCCTTGCCGCAGATGTCGCAGGAGTAAGGCCTCTCGTCCGTGTGCGTTCTTTCGTGGATGAGCAGATTGTAGGACTTGGTGAACTGTCGGTTGCAGAATTTGCATATGAACTGCTTCTTGGACCGCTGGGCTGCCGCTGCCGCCGCCGAGCTGCCCGCCTCCTCCAGCCCTCCTACCGCACCTGCACCTACGCCGCCAGCCGCTCCCGAAATGACACTCATCTTGTCCTGGTACATGGTGGCCTGCAGCGAGTGCAGAGCCGAGTCCGTTTCCGCCTGGCTGAAGGCTGAGGGATTAAACATGAAGCTTCCATAGCTGCTGTCCAAATCTCCCGCCTCGAAGGAGAGAGCATCCTCGCCATCGAGCAACGGTAAGTCGCACTCTGCTGCCAGTTCTGCGGCTGTTGCGCAGTCCAGCGCCGCCTGTTGGCTCGCAGCAGCTGCTGCGGCAGCCGCAGCAGCCGCCACTGCAGCACTAACGGTGCCAATGGAGGGCAGAGGCACGCACTTGGATGTTGTT

 

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