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CRIMIC CR01008

Gene OtopLb

CG3332

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:3278769..3278769	23E1	FBti0206879	83248

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGTTTCTGGAGATCGCCAAACTGAT	AAGAGCGATCGATTACGGAAGGCACT

Images

Construct Design Sequences

sgRNA sequence	TGGATTCAACCTGACCCTGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGTTCCCTGCAGGTTACT	GGGTCAGGTTGAATCCACAT	CTGAGGGAATCGCACACATT	GTCCGACTGATGAAGTGCCA

 

Amplicon 5'

Amplicon 3'

GCTGTTCCCTGCAGGTTACTGAAAAACACAAGAGGTTATATGTACTATAAGAAATAGAAGGTCTAGAAATACGTAATGTGTTAATAAGCCAATTTGGGAAGTATAAGAATAAAAACATATAATATTGGTTAGTTTCTAATGCGATTTGAATGCTGTTCGATGACTTTGTTATGTTAGGGAACCATAAATGTACCCATTTTTGGCAGTCCTCGGCGACAGGAAACCGTTGCCGTCCCTGCGCCCAAAGAGCGGCGGTCGCTGGGTGAGGATCTCTTGGAGGAGCAGCGATGGCCTGCGTGACACCTGCAAGGTGCAGGCGGAGTCTTCGTGGTGCATCCCGACGGTGGGAAGCGGGAGGAGGGCCTGCCGCTGGCTGCCGTGCTCCACGAGATCCAGGTTGGTCTGGGACTTGGCCAGCGGGGAGATGGGATGCGGCGACATGACCGGAATGCTGTGGTGTCCGCAGTGATCACAGGACGTTCGGGCGTAACGGATCACTTGATTCGAGGTGGCGGCCACAGAGTGACGAGGTGTACCAGGTACCGAGGTGGGCAGCGAGCTTGGCGGGGATCTCGAGAACGAACCCGACTTCTTTGGCGCACTTATGGTTATGTATTCGCAGGCATCGGAGGATCTGTGGCTGCTGTTTGAGGAGCGAAATAAGGATATGGGATATCTAATTTTCCGACCAGTGGCACATATACACGCAACCCGACAGGTTCAATGCTTTCCGATGAACTGAATTTTCTATTCCGACTTTGGATTCTTGTGCCTGGTATTATCAATATGTGGATTCAACCTGACCC

CTGAGGGAATCGCACACATTTTCATGTATTTCGCAGCTTTGATTGGTTTTTGATCGATTTCTGCACGTCCATTGAGATATTATCACTCAGCTGGCTAAGAAACCACGCGTGTTCTTTCGGAAATACCTCTAATTTGTATATAAGTGGACAGATAAAAGGGGGTATGTTGTCCCATCAATCAAGGGGTAGATAAGTTCTTGGTGTTTACATAAACATTAAGTGATCACGTTTTCCTTTTGTTGCCTGCAATCAGTGTCTGGAATTTGAGCCAGGGCTTTCACACGAAAAAGAGCTAATGTCATAGAACGACATCTATAGGGGTTTGACGAACCACTGTGATAAGATATAGTACTCCAAAATATTTAGAATGGAAAAGTCTACTGTCTGCTAGTCTGGTACAAATAGCTTTATCTTGAGATCTTCTATAGCCAAAACTCGCTGTGTATATGTGCCACCCCAGTTGAGAATTTGAATGCGAAACTAACTCATTTCCCTTCGATATCACATCGTGGTGGTAATGGACGCGCTGTTTGGTCCTCCACAGATTAATGGGTTCATCGTACATGTCTTTGGATTTCAAGTTAATTAGCGACATTTCCGGGCCGAGTCGCAATTTAATTTATGAAATCAAACGGATCGCGATCACCGATCTCCAAGACGCGATTTTCCGCGCCCTTACAGACTCGTGTCAGTGGGAAGACTGAGACTTTCTGAAGCAGTTACTCCAATTGTCGGAGCTATCGGGCAGACGATATTTGACTAACTGATTAGCGTTATTATTAGCCTGCTATTTTTACCGTACAGGTATGAGTGTGATCCGTGTGTGTAAATACATCTATTGCAGTCCTATTATTGCTCTAATTTATTAGCCTATTGGCACTTCATCAGTCGGAC

 

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