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CRIMIC CR01012

Gene Tomosyn

CG17762

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:12637023..12637023	11B6	FBti0206937	83249

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAACAAGGACGAACTCAGACCAGCA	CCTGGGTCTCTGTAAAGGCAGACAAA

Images

Construct Design Sequences

sgRNA sequence	GATGGAGATTATGTCATCGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCCATCGCATAACTGTGGA	GAGATGGAGATTATGTCATCGGG	TCCATCTCTCTTGCAGCGTG	GCGGCGGGCAAATTAAAAGA

 

Amplicon 5'

Amplicon 3'

CCCCATCGCATAACTGTGGATAGCAGAGCAGCCGGAGCACGAGGACGACTCATGTTACAAAAATGAAAAAGTTCACATTCAAAGGAGTTCTAGATGGATTTCGACAAAGCGTTCAGCCCCAAGCCACACGACAGGAGCAGGAGATTCAGGAGCAACTCAAGGCCGATCACTTTACGTTAAAGAAAGTAAGTTAAAAACATACGATAAATGGGATACGAGTTGAAAGTGCCCATAATCGAAAGGCATCTGTTGCAGCTAGCGGGTGTTTTATTTGGTAGCTGTAATGATTTGAATCTATCATAAGACACACGCGGCTATAACCGTTGTCTAAGAGCTTCAAAATCGATAATAACAAACAATAAAAGTAGTAATTTATAATACTAAATCATACGAGTTAGCGTGATTAGTACGTTTGATCCAATTTCTTCTCTTTATCGCAGACCTTTCGCCATGGCTTTCCATACTCGCCCACATCCTTCGCCTTCGATCCAGTACAAAAGCTACTAGCGATTGGCGACAAATCGGGCTACATTCGCATGTAAGTGTGACTTGAATACAACGTTTTTCTCATTATTTATTAATCTCATCTTTCTATATATGAACTAATTGCAGTTTGGGACGACCTGGCGTCGATGCCCATGCCAAACACGAGGGCGAATCCGAGTGCGCCGTGCTCTTTGCCCAGTTCCTGGTCAACGAGGGCGCATTGGTCACCGTCACCGCCGATGACACCATTCACTTGTGGAGCATTCGCCAAAAGACACCGCGCATTGTGCAGAGTCTGAAGTTTCAGAGGGAGCGGTAAGTTCAAGTCCATTATGTTTGGTCTTATTATGGATATCACGATATCGTAATACTGCCGTCCATAAAACGGGTTATCAATTTGGCTAATTCTCAACATATTCCAACATATGTATATCCCCCGATGACATAATCTCCATCTC

TCCATCTCTCTTGCAGCGTGACTTGCATACACCTGCCTGTGGGCAGCAAATGGCTGTACGTGGGCACCGAGAAGGGAAACATACACGTTGTTCACATAGATACATTTGCACTTAGTGGTTATATTATTAACTGGAATAAAGCAATTGAAGTGTAAGTCCAATTACTCCCTTAGTGACCTAAACCGACCGCATTAAGTTCTAATCACGGGCTTAAACCAATTTGCAGGGTTAGAACTAGTCATCCAGGTGCTGTGATTGCGTTATGTGATAATCCATTGGATGCAAACAAGGTGAATACTTTGTTTCAATGAGGAAAATGCCTCAAGAAGATTTCACTCCCCTTAAACTTAATGCATATATTATATTATTATATTCGATGACTGATGAGATTCTTTCCGCACAGTTGCTTATTGCATTTGAGTGCGGGCTGCTTGTGCTGTGGGATCTGAAGGCGAAATGCGCTGAGCTGCGATGGCAGGCGGCCGAGGCCGTCAAGTCCCTGGCCTGGCACTACGAGGGCAAATATTTTGTGTCCTCGCACACGGATGGCTCCATTTGCTCCTGGCCCACGAAGCCGCAGGCTAAGCCGCAGTCGCAGGTCTGTCCGCATGGTAAGTTGCACAGTCATTATCCATATATAAATATATTTGATTCGATTTATGTATGCTTTAGCTAAAATCAACAAGGATGGCAATGCGGAGAAATGCAAGCCGATATATAAAGTTGACTTGAAGTCCAGCGCAACTGGGTGAGTAATAAGCACTGTCGAATCATTTGTTTACTACTATGCTTTATTTATATTACACGGAGATAGCTGAACAACCTAGGGCGTTATCTTTATTTTTGGAACAATAACCATAAAGCAATTTTACAATAATATTTCAACAATAAGAATCATTGCGCTGCCGGCGTGTCGATAAAACAAATGAATAATGATAACTTGACTCATTAAAAATTTATAATCTTTTAATTTGCCCGCCGC

 

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