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CRIMIC CR01016

Gene CadN

CG7100

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:17730278..17730256	36D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	TAGTACAATTGGCCTGTGGCTTGACG	TTATACCCGTCACGCTTCCACCCATA

Images

Construct Design Sequences

sgRNA sequence	GATCCTGCCCCCTCTACCATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGATAAGGAGCGCGCACAAA	CCATGCCCCTTAGTTACCCC	GCTGGTGTGAATACCGCAAC	TATCGTTCGGTTTGGCGTGA

 

Amplicon 5'

Amplicon 3'

AGATAAGGAGCGCGCACAAAAGTATGCTATGAAAACCGTAATCATAATCGTAAAGCTTAGCATGTATATATTCTGGATCCTACTGATTTGTGTTTCGCATGTGCGGCATACCAAAAAACGAGAATAGAAAGGAAATCTCTGCCTGAAATCGAGCCACTGGAAATTGCTCAAATTAAATGCAAATACCGAAAAATATTTAAATGTTTCAGCGCATTAAGCTATGTAAATTGTTGTCGTTGCCGCTGTCCGGGGACTGACACGCACTTTCCATTCAATAAAACTGCAACCACACAATGGCACATCAATTTACACAGACTCTCGCACTGAGAGGGCGCCGATAAAATGGGATGTGGGGCTGGCAAGTGTCCAGATCACACAACAACATCTGGCATGTTGTCATTAAAGGCCAGGCCAAATAACCCCCCCAAAAAACAGGGGAACTTTCAAGGGGATGGCCTAGAGCACCCTGTGTAACATGTAACATCAGTAAATGAATTAAAAACATATAATTTTTTATATTACATATTTTTACTATGTAGGTGGTATATTTTTTTTAATATTTATATGTAATATTTTTATTAAGAAAATAATCATTATAAAAATTTTTAAAAGGGGACTCTAATCAACATCCCTTCTGCATTACGTTTGGTAATGGTTTATTGCCAGCTTACAATGAAAGCCAAATTGAATCTCAACACCTCTATTAGTTCGCCAGTGTTTGCCGTACAGGAAACATGCTCTTTTCATTGGATTTTCATCCAAGCCAAACCACACACAGAGTATCAACAATGTCGAGCGTTAAATGAATGGCAGTTGGACCACCTCTACGAGCGCGAGCATTTTGCATTTTAATGAGTAAACACAGTGCCGGGCAAAATGGGGGTAACTAAGGGGCATGG

GCTGGTGTGAATACCGCAACGCTGCATATGCAAAGTGTGATTGGTCCGAAAAAAAAGGAGAACAGAAAAATAGTGGTATGGCGAGCAAGGACTTTCCTTCTGTTACCCCACTTTCAATACGAATTTCTGGTGTTTTTCCAAGAATTTTTTTTAACAGCTGCCCAGCTGGGGAGTTTAGAGGACTACGATTTTCCGCGAACAATTTGTATTTGCAATGTGAAAACAAAATGGCCACTGAACACGGCCAATCCGCAGGACATTTCCCCAATCTGTCCCCTTTTTGGCACACCTGCTGTTTGATTTGTGTTTTTCTCACTCGAAAAATGGTCTCTGGTTTTTCTCTTTTCGTTTTCATCAAATAGCCAATGCGGTTAACAGTGAAAAACTCTGTGCTAAATATGCTTTTCTGTTGTTTTCCCGCTGCTCTTATTTAAGTCACACAATTTACGCTACCAAAAAGTGAACGGGGAGGAAAATTATTTATTTCGCTTGGACAAGCAAGTGTTTATTGATTTAGCTCAGTAAAAGTTTGTTCAGCGATTTTTATCTGTATAAACATTCAGAAGCCTACGTCTGCATAAATACCGTAATAAAAGATACTAGATTGTGTTCAAATCATTTAATATGTCCCTTAATTTTATTACATTTTTTTTAATGTTCATCACGAATTATGCAACATGCGATAAAAAAAGCTTTACTCAACCATATGAAAAATTAATCATTTTTATTAATGTGATTCTTTGTTGCTTTATGCATCATTATAATGTGACAAATGCGTGTGGCTTTGATGATGACCAAAACATGTGTTTAAAAGCGTGTTAAATATTTATATATCTTAGTCTGACATCTGACGTCAATGTCGATGCGTTTGGCTAATTTAGTTAGAGCTTGGCCATAATCAAATTCAATGACATGTGAAATTTTCACGGATCAGTTCACGCCAAACCGAACGATA

 

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