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CRIMIC CR01017

Gene Oatp33Eb

CG6417

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:12449493..12449471	33E5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTAGCACTGCTTGGACACATGTTGC	TGTGCATAGGCACTGACTGGAGCAAT

Images

Construct Design Sequences

sgRNA sequence	TTAAGAACGGACTTTCATTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTCAGTTTGCTCCCGTTC	AAAAGCCACTTCCGTTCGAA	AGTCCGTTCTTAATCATACGTCA	CATAACTGGCGGCTTGAGGA

 

Amplicon 5'

Amplicon 3'

TCCTCAGTTTGCTCCCGTTCCTCATTTCGAATAGACGTTAGTGCTGTGTTAACGCGGCTAAGATGCCAACGTACCACGGCCACTGACTAATGTAATCAATAGAGAATACAACAAGCTAGTGAATTGAATTGAAGTGGTGTAGTGAACATAAACGAATCCGAAATCGAATCGAATACGGAATATGGCGCATAATACGCGCAACTCCGATTTATCGCAGATAGCTGTCAATGCCAACAATATACCCGATGGTGGGTGACCCAAATCCCTGATTTCCCCTTTACGTATACTTGATATAGAATTTCTACCCCCTTCCCACCACCAAACAGACAGCGTCGATTGTGGCATCAAGGGTCTGGGATGGTGTCGCGGTCCCGGATGCCAGAAATATGCCCGACTGCGCACCTTCATCCTGGTACTGGCCATCTCGGGAACCCTACAGGGCGCCTGCGAATCCTACTTCCGCGTGTCGGCCAAACAGGCTTCCTTTCAGTTCGGTTGGAATCCACTGATTGTGGGTGAGTAAGCGAGCAGCATTATCGCAAAAATGGGAAAATGCTAACCTGACCAGGGAAATATAATTGAGGTGTTGGCCCGGTTCTATGTGAAGATTTCAGTTATCTTATCAGTTTTGCGATATTCTTGACGATTGCCAGTGTCTGGGAGCTGGGGGTGCTCTCTATTTTCCAGTAGTTTTCTTAAATATCTTTTTAAATTAAGAGGTATAAAGTAAATTACAAAATATTAAAACAAAACTGAGCTGTATTATGTTATGTATGTATGTTTATCAAGGTAATCAATTAAATAATATTCTAACTACAATATTTAGTTTTACCTTTGTACACTGTTTGGTGTATTGGTATTTAGGTATCTATTCGTCATTGTTTTGATAAGGAGGGAATACATTATGGTAGTAAAAATTGCCGCGTAATACATTTTTTTTAGGTTTTTGCAAATCAACTGAAAAAAGAATTCGAACGGAAGTGGCTTTT

AGTCCGTTCTTAATCATACGTCATATCTCAGTTGGTAAACTGGTTATATAGAGTTTGTAAGGCGAATTCCACGAAAGCGTATTACTAACAAACTGTTTTATTCTTTAAACATACAGATTGGCTGCTGGTGGCCAGTGGATTATTCCAGGCGGTCTTTGCGTTGGCTTTCGCCTACTGGGCGGACGTCTATCATCCCATCAAATGGCTGGTGGGAACTCTGATGCTGCAGGCGGTCACCTGTGTGGTGGCTGTGATTCCCTCCATTATGAACTTGTGAGTAACTTGTAAAAAAAAAATACTTCATTAGCATTTGATTTCTAAAAAAAATAATTTCTTACAACTCTCTTGCAGTGCCGATGGCAACCAGGCAAAGGATGCTCTGCTGGATGCCAATATGTGCGCCACATCCTTGGCACAGTTCCAAAGGCTCACACTGACCGAGGCCCAGAGCAGCAGCCTAACCCTTGCGATGCTCTTCGTTCTTCAGCTGGCCTTGGGAATGGGAGGATTGGCCTACTACACGCTGGGGGTTAGCTACATCGACGACAACTCCCTGTCCCAAGATAGTCCGGCGGTGATAGGTGCTGCTCTGGCTGCGCGCGTATTTGGCCAGCAGGCTGGATCCTTCCTAGTGCTTGGCGTTGGTCTCACCCATGTGGGTTGGTGGCTCGGTTGGGTGATACTGGCACCCTTCATTTTCGTGGGCGCTGTGCTCCTGGGACTGTTTCCCAAACGACTGCCAAAGACCGTCATTCACCAGGCGGCGCAACGGATCATTGAGCAGTCCAGCATGCGTAGGTTTGGCAGCCAGTTCTCAACATATATGGATGACTCCGATTTTTGGCCATCTCTGAAGAGGCTATTCAATAACCGCCTACTGATGTTCAATCTGTTGAGCATCATGTGCGTGCAGAGTGCAGTCGTGAACTATGGCCTCCAGGAGGAGAGCTATCTGCAGAGCAGATTCTTTTTGCCCTACAACGAACAGGATGGCTTGGCGGCAGAGTGGAGATCGGCATTCGTTTCCTATTTCCTCAAGCCGCCAGTTATG

 

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