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CRIMIC CR01018

Gene Ddr

CG33531

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6278160..6278168	26C1	FBti0202661	81157

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGCCGCACACTGGGCTAATATTCTT	TTGATGGCTGGGATATTCTGTGTGCG

Images

Construct Design Sequences

sgRNA sequence	TAACCCTTGTCTGGTGCGCCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCATTTCGCCGTCAAAGT	CACCAGACAAGGGTTAGGCC	TCGGTGCTCAGCTTTGACTT	ACACGCAGGAAACGAGAGAA

 

Amplicon 5'

Amplicon 3'

CTGCATTTCGCCGTCAAAGTTTCGCAAAATAGTTTCCTCCCAGGGAGATGTTTTCCCGGGGTCTCGCCACCTTTGTTTATTCGGCCAAATATAACATGCTCAAAGCCACCAAGCATTTGCCAGGCCGTCGCCTTTGAGTTGGCATACTTTTTCCACTTGCCTGGCAATCATCATCCGTCCTCCGGAGACGGAAAATGCTGTAGAGTGGTCGGTCCATTGTTATAATGGATATGCTGGAATCCAAATCGAGATCATCCATCAATTACGCACAGCACACTTAGTGCCACAGTTTCTGCATTTCCCGCCAGTTGTAAGAGTTTTGTATGTTTTTCAATAATTAAAAATAATTTATTTATGTTAATATTGAGGGAGGTTCCAAATAGTAGAAGTTGACTGATTGCAACATCTACTGCCAACTTTGGCCGTCTGTGCATAATTAAGCTATTGCGTTCTCAACAAATGAGTTTTCAGAACTGCAGAAAGAATGGGCTAAATAAGTAAACAGAAAAACCATGCTCTGGAAGCATTGAACACATAAATATTGTTTATGTAAGTAAACATTACGAGGGCAATGCGAGCGAAATAGATGAATATTTGTTGACTTCACTCGCTGCGATAATGAAACCAATTTGTTTATCAGTAAGCGTGGCGCGAAATGTTTTTTATAGTGCCGAATAATACAGTTTTTCATTCAAAAGCTGTTTGTATAAAGTTCAGCTGTGTATCAATCATTTTTGAATATTTCCTGTTTTTGTCTAGCATCCCATTTTCCAAAACTATCTGAGCAGCCCTCGCAGTCTTGGGATCTTCTTCGAGAAGAAACTAAGAAAAGGGTCACTTCGAGCATGGGTATCCATATCCATATCTGCAGATGGCGCGATTGACCGCCTCCAAAGCAGCATTCTCGGTGGGAGTGGACGAGTGGGAATGGAATTGGGAATGAGAGTGGGAGTGGGCCTAACCCTTGTCTGGTG

TCGGTGCTCAGCTTTGACTTATGGTGCTTAAGCACTGAGTGATTGCATCTAATTATTCACAGAATGATAATATTCTCGATATTTTGTGGAAGCAGCAACAACTTGCACCGAGCCGAGGGGATGACGATGATGATGATGTCTGCGATGTCTCAACGACCTATTCATGACCCCATCGCGGTCCCTTGGCTGGTGGGAGGAGGTGATAAGGGGGGCGGGGCCCCATCATTCTTGGAGCCGTGATGATAATTGCGCTTTTCATTCCCTCTCACAGATTGATGGCGAGTGAAAATTGAGTTGCTGAAATGATAGCAGCATAGTATCGCTTCCAATTTCCCGAGCTTTGTCCGCGGCATTCGGCAATTAATTTACGCATAGGGAAAGGTCTCTTGATTTGTTTTCCAATTACGGAATTCCACTGCTTTGTTGAATATCTCCTTGATTGAATATCTCGTTCTTTAATTAGTTGTAATTTCTCATCTTTTTTGACCAGATTTTCTTTTGCTTTTCACAGGAACCTGCAAACAGGCCTTGGGCATGGAGTCTGGCGCCATCGCCGACTTTCAAATAACGGCCAGTTCGGCCCATGATATGGGAAATGTGGGACCACAGCATGCCAGGTAAGCTAAGCTCCCCAATCTGGCCCCTATATCATGGCACACACGTGTCTCTGATGCTGCCTGAAAATGATAAACATCCATTAAATTTGACTCATAATATGACGAATACGAGTGTGCCTGTGGGTTTGTATAATGTGTAATGTGCACACACACACACACACACATAAGACATTCTCTCGTTTCCTGCGTGT

 

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