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CRIMIC CR01019

Gene Pde11

CG34341

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18545256..18545277	36F6	FBti0202662	81158

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTGAGAAGGTGGCAAGCAAGAAAG	GTCGCTGGCAATTGACATGGCTTCTT

Images

Construct Design Sequences

sgRNA sequence	TATCTACAACACAGGAGAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTAACACATGGCAAACGCG	GGAGACCTTGCAGATGCTTAAC	ACTTTTCGTTGGTCTTTCGCTT	GCATTTGGCTGCACTCGAAA

 

Amplicon 5'

Amplicon 3'

TGTAACACATGGCAAACGCGGCGTATACGTAACGCCATAAATAACATTGGCAGTTGGGGCTCCCCAAAAGTGTTTCAGTTAACATTAACCGGAAATATTGGCAATTTCCTTGAAATATTGACTTCGTGTGGGCTTGCTGCAAAGTGCCTGGCGTCTGTAATGCCAGCATTGAGCGCCTGATTAAGTGCTAAAAATAGATATAAATACGACAACGAGTAACCCACTGATACATGTCTCAAGGATGCGATAGAAGGAGTCCTTACTCATCTCTCTCTCTCTCTCTCTCTCTATTGCCCCCATTTTTGTTTGTGCCCCCAGTTATTTATTCTATAGAAAATATGATTTATGTGCTGCTTTTCGACTTACACAAAAGAAATAAACAAACGACTTGTTCCTGTCGCTTGACAATTCGACTTTCGGGCTAAGCTGGTATACAACAAATATCAAGGATATTTCGTTTTCTTAAATACGTTAGAGTAGTCTATTTTGGGCTAGATTACATACGGAAATATTTTCAGTTGGATGGCTTTATATTATTCACAACTTTTTGGGAAATTTATTTAATTATTAATTTACATATCTATGCAACTATACATTTGTATCCTCTTTTGAGTAAAACAGAAAATGTATTACTGATTTGCAGCCAGCTTCAATACCCATTTTGTTTCTGGTATGAAAATTATTTATATGTTTACCATATGGGTTCGATAACGTTTATATAAAAATTGCTTTTTATGCCATCCGAGGTTTTTCTTTTCGATGTAATTTATAGCAGTACAAATTTAATTGGATTTTATGCTACCATACTTTTCCTGTGGGTTCTTTCGCTTTTCCATTTAAAATTCACTTTAATATTAATTTCAGCTTTTCCCCCATGATTTGTGCCCCCAAGAATTTCCCTGTGCAGACATAAATTTCCATGTTAAGCATCTGCAAGGTCTCC

ACTTTTCGTTGGTCTTTCGCTTCTAAGCTTGCCAATAAGTTGAAAGTCGGCTACTTAGTGCTGCAGTGGGGGATTTGTTCAGATGGAATGACTAGAACGCCACACACATGCCACCCATGTAATGCAGCCTCCGAGGAAGGGAATTTAATCTTTTAAGCTGCATTTGCCTTGCACACATCCAACATCCAACATGCAACATGCTTAAGCTGCTGCAAAACATTTGGCACAGAGCAGGAGCACTTTTCCACTCCCCGCTGAATTCCTTTGGCCATTTCCTGCACTCGAAGTCAAATGACAACTTGACAGAGTCGTGTGGGAGGTGTTCTGTAGCACCCGTTTTCCAAGTTTCCCCATTTTCCCGCCGCCGCCAACACTTTGCTGCGGTTTGATGCGTCAGCTTTATGTAAGACATTCATATGGGCCCCTCAACCAACATCCAACCCCGAATCCCTCTTCGCTACCATCGTCTCAGTCAGGCGAATGGCAAATTTTTATACAACATTTGTAATGGAATTTTACTTTTAGCAGGAACAAGCAAGGATCAAGAGTTCTTGGCGATATGACAACAAATTTAGTACTCTTCGTGGGATGGCAATATTTGATTTACATGCTAATCCAAATGGGAATACATTTAGTGGAGTACTGAAAAGCGTCGGAACAGAAGAAATGGTGCAGGAATAATTTTTCAATAGATTAATATTCGGAAAATATAAGCATATTTGCGATGAAAAGATAGTAATTGTATCTTTTCTATTTCAAAGTATTTAAATTAGAATTCTATCAGGTTTAAACATATCATAGATCCATAAATATTATGTAATAATTTCTGTTCCTTTTCTTAGCAACAATATGACCTCAATTTATAGGGTATGATTACCACATACAATACCATATATCTGCACACTCTAGCTCGCATATATGAGCCAGTGTGTAAATGGCATTGTTGCTTTCGAGTGCAGCCAAATGC

 

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