CR01020

CRIMIC CR01020

Gene smal

CG34380

Information

Donor Location Cytology FBti Stock number
pM37-p12L:6192346..619236826B10Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK GTGATGTGGTATGGCATTGGAGTCTG GAACTTTGCTCGCTCGATGAGGGAAA

Images

Construct Design Sequences

sgRNA sequence AGCGGGCGACTGTCAGAGGCAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTGCCACAATATAGCCCCT GTCGCCCGCTGACATTTT CAGGCGGATCTCCTTGGGA CATTGAACAGCCGCTTGAGC
 
Amplicon 5' Amplicon 3'
GCTGCCACAATATAGCCCCTGCCCCCCCAATCCAGAAACGAAAATGAAATCTTTCTGCCATAATATTTATAGTTAATCAAGCAAGCCAAATACATAAATACAAATCTATAAACACCCGAAATGAACGGGTTCCAGAACTCGACTGCCTCCGTTGGCTGCCCTAGCAGTATTTGCACTTATCCCACCCCCCCAATCCCCGCCAGAAAAGCAAACACATGCCAAAGGAGCACTCTGCCGAAGGTTGTTGTTCCTGTTGTTGTTGTTGGGGCTAGACACATCGAAGCGAGTTGAGTTAGCAAAACATGCAACAATTTTCCAAGTGAATGCACTTGCAATATGCAGTGCATGTCGGATTGAGAGTCGGGGTGTGAGATCTGGCTTTTGACCCAACCTGGCCCATGCCATCCAGACAACCGAGCATCCCAGCATCTGAGCACTTCGCTCTTCATTTGCGTAGCCGGCTATCAAACCCAATCAAGTGTGAATGCACTGGAAGCTAACTTTTCGCCGAGATGGGAAAACTTGGAGACCATATACCACACACTATGTGCAGTATTCATATGACTTCTGAATATAAAATGGCAAATAATATTGTCCCACAGAGCTGCAATTAACTTAGACAGTCAAAAGACCTAAAAATAAGATTCATTTACACTTCAGTGTAGATGGATGTACATACATATAGTAACTTCACAAGAGCTCACTTTGTATTGGTTGGCTGACTTCTTGCCGTGTGATTATTTATAAACTTCTAAACGAATGCACGCAACCTTGCTCTGGAGGAGATCAAAAGTGGGATTGGGACTGGGATTGGTCCGTGGTGAGCCTGCTGGGTCCGGGCAACAAAAGTCATGTCAAATCAGTGTTGATTCTGTCGAAAATGTCAGCGGGCGAC CAGGCGGATCTCCTTGGGACTCCCTCCATATCAAAAGGCAAATGAGAGGGAGGGTGGAGTCTGCGCCCAGGGCAAATGCAATTGGTGGGCTATAAATTAAGTAAGGTAGTTGACGTTCTGCCAGTCCAGAGTTGACTTTGCCAATGATTAAGTGTTTGGTTGACTCAAAATGAGATATTCTTTGAGACGATATGCCCTGCAAATGCGTCAGACATGAAGCATTTATAAGCATCGTCAAACTCGAACTGACACACTTGAGGCTTTTTGATATATTCGATTGCATTGCGCAGACCACAATTCACTGGCTCTATATTTAAATACTTACGTCGTCATTAATTAATGTTAAAAATACAATTTCCTCTCATTTCCAGCCACGTGCAACGTGCCCCTGGGCATGGAGTCGTTACTCATAACAGATGCTCAAATCACAGCGAGCTCAGCTCATGACATGGCCTTCGTTGGACCTCAGCATGCAAGGTAAGTTGGCCAAAGCTGATGGAGGAGTTTAACCCAAAGCTGCAGGTTAAAGGAAAAGTACCTGGGGATATAAAAAGAATCGTAGCTGTGGGGCAAAAGAACGGGTTTTACGATTATTCCTTGGGTAACTTCTCAGCAGCAAAGTATTAGCCTTTTAAGACTGATCATCGATATCGTCTGCGATCAGGTTTTTGAGATTACAAGACTTTATATTACGTTTACGATTGGAACCCTGCCTGTGCCACAGCTCCCGGCTCATTAACCATTTAACCCCCAGATGGAAAAAGTTGGGGGGATGGGAAAATGGAGGCTTATCTGCGATGCCGCTGCCCTGCTTGTCCATTTAATTGCTAATTTTATAGACAACTTCGTTAGGCGATAAGGCAGAGTCAATAAAATAGAAAACTTTGCCCACCGCTCAAGCGGCTGTTCAATG
 
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